cDNA Cloning of ACC Synthase and ACC Oxidase Genes in Cucumber Fruit and Their Differential Expression by Wounding and Auxin.

Shiomi, Shinjiro; Yamamoto, Mikihiro; Ono, T.; Kakiuchi, Katsuya; Nakamoto, Junichi; Nakatsuka, Akira; Kubo, Yasutaka; Nakamura, Reinosuke; Inaba, Akitsugu; Imaseki, Hidemasa

doi:10.2503/jjshs.67.685

Cited by 29 publications

(15 citation statements)

References 18 publications

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“…Auxin increases the CS-ACS1 mRNA accumulation in the shoot apices and fruits of the cucumber (Trebitsh et al, 1997;Shiomi et al, 1998). It has also been shown that CsIAA1 mRNA accumulates asymmetrically across the transition zone of cucumber seedlings grown in a horizontal position Kamada et al, 2000) and that incubation of transition zone sections in IAA-containing buffer increases their CsIAA1 mRNA accumulation (Kamada et al, 2003).…”

Section: Ethylene Evolution and Acs Mrna Accumulation In Cucumber Seementioning

confidence: 96%

“…Four ACS cDNAs have been identified in the cucumber (Kamachi et al, 1997;Trebitsh et al, 1997;Shiomi et al, 1998). Their names have not been standardized, and thus in this study they have been denoted according to the nomenclatures in Yamasaki et al (2003) as follows: CS-ACS1 (accession number U59813 and AB006805), CS-ACS2 (Kamachi et al, 1997), CS-ACS3 (accession number AB006803) and CS-ACS4 (accession number AB006804).…”

Section: Quantitative Rt-pcr and Northern Blot Analysismentioning

confidence: 99%

“…Four ACS cDNAs have been identified in the cucumber (Kamachi et al, 1997;Trebitsh et al, 1997;Shiomi et al, 1998). To examine the involvement of the cucumber ACS genes in peg formation, the mRNA accumulations of the four CS-ACS genes were compared in the concave and convex sides of the transition zone of 24-h-old seedlings (Fig.…”

Section: Ethylene Evolution and Acs Mrna Accumulation In Cucumber Seementioning

confidence: 99%

“…Auxin can increase the expression of specific ACS genes (Huang et al, 1991;Nakagawa et al, 1991). Four ACS cDNAs have been identified in the cucumber (Kamachi et al, 1997;Trebitsh et al, 1997;Shiomi et al, 1998). However, while the accumulation of their mRNAs in shoot apices including floral buds (Kamachi et al, 1997;Trebitsh et al, 1997;Yamasaki et al, 2001Yamasaki et al, , 2003 (Shiomi et al, 1998;Mathooko et al, 1999) has been investigated, their expression in cucumber seedlings has not been analysed.…”

Section: Introductionmentioning

confidence: 99%

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Possible Involvement of CS-ACS1 and Ethylene in Auxin-induced Peg Formation of Cucumber Seedlings

et al. 2004

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Background and Aims Cucumber (Cucumis sativus) seedlings develop a peg on the concave side of the gravitropically bending transition zone between the hypocotyl and the root after seed germination. Peg initiation occurs in response to auxin when its levels in the concave side of the transition zone exceed a particular threshold through the graviresponse. Ethylene also plays an important role in peg formation, but its relationship to auxin in this event is not understood. Here, the role ethylene plays in auxin-induced peg formation is studied.Methods Peg formation of cucumber seedlings exposed to ethylene at different stages of growth or during exogenous auxin treatment was observed. In addition, ethylene evolution from the concave and convex sides of the transition zone was compared and their transcription of CS-ACS (1-aminocyclopropane-1-carboxylic acid synthase) genes was analysed by RT-PCR and in situ hybridization.Key Results Seedlings treated with ethylene after peg initiation produced an enlarged peg, whereas ethylene treatment before peg initiation inhibited peg formation. Ethylene also promoted the development of the peg in the auxin-treated seedlings. Furthermore, the concave side of the transition zone at peg initiation produced more ethylene and CS-ACS1 mRNA than the convex side.Conclusions Since CS-ACS1 is an auxin-inducible gene, the greater abundance of auxin in the concave side of the transition zone causes peg initiation and increases CS-ACS1-mediated ethylene biosynthesis, which then facilitates peg development.

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Section: Ethylene Evolution and Acs Mrna Accumulation In Cucumber Seementioning

confidence: 96%

Section: Quantitative Rt-pcr and Northern Blot Analysismentioning

confidence: 99%

Section: Ethylene Evolution and Acs Mrna Accumulation In Cucumber Seementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Possible Involvement of CS-ACS1 and Ethylene in Auxin-induced Peg Formation of Cucumber Seedlings

et al. 2004

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“…2003). In cucumber plants, four of the ACC synthase genes have been identified (Kamachi et al 1997;Trebitsh et al 1997;Shiomi et al 1998). Even though CS-ACS2 mRNA accumulation is correlated with femaleness in cucumbers (Kamachi et al 1997;Yamasaki et al 2000Yamasaki et al , 2001Yamasaki et al , 2003, further study is necessary to clarify the positional relationship between cells producing and receiving ethylene in determining the sexuality of cucumber flowers.…”

Section: Ethylene Production and Perceptionmentioning

confidence: 98%

Characterization of ethylene effects on sex determination in cucumber plants

Yamasaki

Fujii

Takahashi

2003

Sexual Plant Reproduction

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Sex differentiation in cucumber plants (Cucumis sativus L.) appears to be determined by the selective arrest of the stamen or pistil primordia. We investigated the influence of an ethylene-releasing agent (ethephon) or an inhibitor of ethylene biosynthesis (aminoethoxyvinyl glycine) on sex differentiation in different developmental stages of flower buds. These treatments influence sex determination only at the stamen primordia differentiation stage in both monoecious and gynoecious cucumbers. To clarify the relationships between the ethylene-producing tissues and the ethylene-perceiving tissues in inducing female flowers in the cucumber, we examined the localization of mRNA accumulation of both the ACC synthase gene (CS-ACS2) and the ethylene-receptorrelated genes (CS-ETR1, CS-ETR2, and CS-ERS) in flower buds by in situ hybridization analysis. CS-ACS2 mRNA was detected in the pistil primordia of gynoecious cucumbers, whereas it was located in the tissues just below the pistil primordia and at the adaxial side of the petals in monoecious cucumbers. In flower buds of andromonoecious cucumbers, only CS-ETR1 mRNA was detected, and was located in the pistil primordia. The localization of the mRNAs of the three ethylene-receptorrelated genes in the flower buds of monoecious and gynoecious cucumbers overlap but are not identical. We discuss the relationship between the mRNA accumulation patterns and sex expression in cucumber plants.

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