cDNA cloning of human DEC-205, a putative antigen-uptake receptor on dendritic cells

Kato, Masato; Neil, Teresa K.; Clark, Georgina J.; Morris, Christine M.; Sorg, Rüdiger V.; Hart, Derek N. J.

doi:10.1007/s002510050381

Cited by 63 publications

(39 citation statements)

References 33 publications

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“…Because these DC subsets have the potential to dictate the type of immune response generated (5, 10 -13, 30), the search has begun for DC subset-specific molecules that could be used to manipulate immune reactivity. Indeed, several such molecules have already been identified (31)(32)(33)(34)(35)(36). Our RDA between just two DC subsets has already revealed two novel cell surface molecules, suggesting that more such molecules are yet to be discovered.…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning of F4/80-Like-Receptor, a Seven-Span Membrane Protein Expressed Differentially by Dendritic Cell and Monocyte-Macrophage Subpopulations

Caminschi

Lucas

O’Keeffe

et al. 2001

The Journal of Immunology

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A novel dendritic cell (DC) surface molecule termed F4/80-like-receptor (FIRE) has been selected based on its differential expression between DC subsets. The gene encoding FIRE has been cloned and sequenced, and mAbs specific for FIRE have been produced. FIRE is a seven-transmembrane-spanning molecule with two epidermal growth factor-like domains in the extracellular region. It is a novel member of the epidermal growth factor/transmembrane-7 protein subfamily and shows similarity to the macrophage marker F4/80. FIRE is expressed by CD8− DC, but not by CD8+ DC, and it is down-regulated on DC activation. It is expressed by blood monocytes and by some tissue macrophages, but not by most macrophage cell lines or by lymphoid cells. FIRE is a useful marker of myeloid cells with a DC developmental potential.

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Section: Discussionmentioning

confidence: 99%

Molecular Cloning of F4/80-Like-Receptor, a Seven-Span Membrane Protein Expressed Differentially by Dendritic Cell and Monocyte-Macrophage Subpopulations

Caminschi

Lucas

O’Keeffe

et al. 2001

The Journal of Immunology

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“…During this recycling process, bound ligand is released from the MR in the acidic environment of the endosomes for subsequent processing, resulting in high amounts of internalized Ags and efficient Ag presentation by DCs (16,17). Another multilectin endocytic receptor is DEC-205, which contains 10 CRDs and is expressed both by DCs and by thymic epithelial cells (18,19). To date, the mannosylated ligands recognized by DEC-205 are not identified.…”

Section: The Dendritic Cell-specific Adhesion Receptor Dc-sign Internmentioning

confidence: 99%

The Dendritic Cell-Specific Adhesion Receptor DC-SIGN Internalizes Antigen for Presentation to T Cells

Engering¹,

Geijtenbeek²,

Vliet³

et al. 2002

The Journal of Immunology

569

500

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Dendritic cells (DCs) capture Ags or viruses in peripheral tissue to transport them to lymphoid organs to induce cellular T cell responses. Recently, a DC-specific C-type lectin was identified, DC-specific ICAM-grabbing non-integrin (DC-SIGN), that functions as cell adhesion receptor mediating both DC migration and T cell activation. DC-SIGN also functions as an HIV-1R that captures HIVgp120 and facilitates DC-induced HIV transmission of T cells. Internalization motifs in the cytoplasmic tail of DC-SIGN hint to a function of DC-SIGN as endocytic receptor. In this study we demonstrate that on DCs DC-SIGN is rapidly internalized upon binding of soluble ligand. Mutating a putative internalization motif in the cytoplasmic tail reduces ligand-induced internalization. Detailed analysis using ratio fluorescence imaging and electron microscopy showed that DC-SIGN-ligand complexes are targeted to late endosomes/lysosomes. Moreover, ligands internalized by DC-SIGN are efficiently processed and presented to CD4+ T cells. The distinct pattern of expression of C-type lectins on DCs in situ and their nonoverlapping Ag recognition profile hint to selective functions of these receptors to allow a DC to recognize a wide variety of Ags and to process these to induce T cell activation. These data point to a novel function of the adhesion receptor DC-SIGN as an efficient DC-specific Ag receptor that can be used as a target to induce viral and antitumor immunity.

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“…3Ј-Rapid Amplification of cDNA Ends-The 3Ј-end of DEC-205 mRNA was obtained by 3Ј-RACE, which was performed as described previously (17). Briefly, L428 mRNA was reverse-transcribed with an oligo(dT) adaptor primer.…”

Section: Methodsmentioning

confidence: 99%

“…TARC) (14), which are associated with APCs. L428 cells have been used successfully in our laboratory to produce monoclonal antibodies (mAb) against DC differentiation antigens such as CMRF-44 (15) and CMRF-56 (16) and to clone the DC-associated molecules such as DEC-205 type I transmembrane multilectin receptor (17) and the adenosylhomocysteine hydrolase-like molecule DCAL/AHCYL-1 (18).…”

mentioning

confidence: 99%

Hodgkin's Lymphoma Cell Lines Express a Fusion Protein Encoded by Intergenically Spliced mRNA for the Multilectin Receptor DEC-205 (CD205) and a Novel C-type Lectin Receptor DCL-1

Kato

Khan

Gonzalez

et al. 2003

Journal of Biological Chemistry

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Classic Hodgkin's lymphoma (HL)1 is a common malignant lymphoma characterized by the presence of a small population (Ͻ1%) of putative malignant cells, the morphologically distinct Hodgkin and Reed-Sternberg (HRS) cells. Recent advances in cell isolation techniques and molecular biology has identified Ig gene rearrangements within the majority of individual HRS cells, suggesting their B cell origin (1). These are surrounded by a large population of apparently non-malignant lymphocytes and histiocytes, whose proliferation is likely to be mediated by the wide range of cytokines and chemokines released by the HRS cells (reviewed in Refs. 2 and 3).HRS cells have many characteristics in common with antigen-presenting cells (APCs) such as activated B cells and dendritic cells (DCs) (4). Indeed, the HRS cell lines (L428, HDLM-2, and/or KM-H2) express cell surface molecules required for costimulation/proliferation of T cells (major histocompatibility complex class II, CD40, CD80, and CD86) (5-7), cell adhesion molecules involved in DC-T cell interactions (LFA-1, CD11c, and ICAM-1-3) (8, 9), and the DC-associated molecules (CD83 and fascin) (6, 10). They also produce inflammatory cytokines (e.g. tumor necrosis factor-␣ and lymphotoxin) (11), non-inflammatory cytokines (e.g. granulocyte macrophage-colony stimulating factor and interleukins 5 and 13) (12, 13), and chemokines (e.g. TARC) (14), which are associated with APCs. L428 cells have been used successfully in our laboratory to produce monoclonal antibodies (mAb) against DC differentiation antigens such as CMRF-44 (15) and and to clone the DC-associated molecules such as DEC-205 type I transmembrane multilectin receptor (17) and the adenosylhomocysteine hydrolase-like molecule DCAL/AHCYL-1 (18).We have investigated cell surface molecules on HRS cell lines with a view to identifying novel molecules related to APC function. These molecules might also be candidate targets for antibody-based HL immunotherapy. Indeed, CD20, CD25, and CD30 reagents (markers for B cells and activated lymphocytes) have been investigated in this regard (19 -21), but molecules more restricted to HRS cells might be preferred as targets for more specific therapeutics.During the cloning of DEC-205 from the L428 cell line by 3Ј-rapid amplification of cDNA ends (RACE) (17), we discovered an alternatively spliced novel DEC-205 mRNA. This mRNA encodes the intact DEC-205 ectodomain but included unique sequences encoding for an additional carbohydrate recognition domain (CRD) and a transmembrane (TM) and a cytoplasmic (CP) domain derived from a newly identified type I transmembrane C-type lectin DCL-1. A partial cDNA sequence

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cDNA cloning of human DEC-205, a putative antigen-uptake receptor on dendritic cells

Cited by 63 publications

References 33 publications

Molecular Cloning of F4/80-Like-Receptor, a Seven-Span Membrane Protein Expressed Differentially by Dendritic Cell and Monocyte-Macrophage Subpopulations

Molecular Cloning of F4/80-Like-Receptor, a Seven-Span Membrane Protein Expressed Differentially by Dendritic Cell and Monocyte-Macrophage Subpopulations

The Dendritic Cell-Specific Adhesion Receptor DC-SIGN Internalizes Antigen for Presentation to T Cells

Hodgkin's Lymphoma Cell Lines Express a Fusion Protein Encoded by Intergenically Spliced mRNA for the Multilectin Receptor DEC-205 (CD205) and a Novel C-type Lectin Receptor DCL-1

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