cDNA cloning, sequencing and in situ localization of a transcript specific to both sublingual demilune cells and parotid intercalated duct cells in mouse salivary glands

Bekhor, Isaac; Wen, Yi; Shi, Songtao; Hsieh, Chih-Hsin; Denny, Patricia A.; Denny, Paul

doi:10.1016/0003-9969(94)90052-3

Cited by 18 publications

(25 citation statements)

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“…1,16-30 kDa), based on their common reactivity with polyclonal antibodies to the prominent component, Protein BI (26 kDa) (Ball and Redman, 1984;Ball et al, 1988a Redman, 1984, Ball et all, 1988a, 1993 There is another protein that has much the same tissue distribution and ultrastructural localization as the BI proteins, but which is encoded by an unrelated gene, In rats, this protein is called common salivary protein I (CSPI ), because it is present in all three major salivary glands in one or more differently glycosylated forms (Girard et al, 1993). The homologous transcript in mice, P20, exhibits a similar gland-and cell-type distribution, though its abundance in neonatal and adult submandibular glands appears to be much less in mice than in rats (Bekhor et al, 1994) The submandibular gland-specific mucin secreted by mature acinar cells.is also detectable in the secretory granules of the proacinar cells (Type 111) in mice (Denny et al, 1988) (4) SECRETORY PROTEINS FOUND IN TYPE I CELLS Type I cells secrete in response to cholinergic stimulation and produce a protein that is called Protein C (89 kDa) (Ball and Redman, 1984;Ball et alc, 1988a,b). No immunoreactivity for Protein C is seen in early, differen- tiated Type III cells (Fig.…”

Section: (6) Origin Of the Three Major Types Of Salivary Glandsmentioning

confidence: 99%

“…In contrast, CSPI message accumulation then increases sharply in the male, and at 45 days and at three months, is similar to the level seen in the neonate. Mouse P20 (CSPI homolog) transcripts show a uniformly low abundance throughout development and into adulthood in both males and females (Bekhor et al, 1994). As discussed below, the adult expression of both Bl proteins and CSPI is primarily in the intercalated ducts.…”

Section: (6) Origin Of the Three Major Types Of Salivary Glandsmentioning

confidence: 99%

“…In both the neonate and adults, Western blots show the ma jor BI-reactive component at 27 kDa, another BI-reactive band at 18 kDa, and Protein D at 175 kDa (Ball et al, 1988a). Still another phenotypic link between the submandibular and sublingual glands is the presence of CSPI (and P20) in the serous demilunes of the sublingual glands; indeed, the mRNA abundance in the sublingual is more than 10-fold higher than in the submandibular gland, and appears to be the same in males and females of both rats and mice (Girard et al, 1993;Bekhor et al, 1994).…”

Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

“…The anti-Bl antiserum that recognizes the B -IP of the neonatal rat submandibular gland also reacts with both mouse and rat PSP. Like the BI-immunoreactive proteins, transcripts of CSPI in rats and P20 in mice are relatively abundant in the adult parotid gland (Girard et al, 1993;Bekhor et al, 1994). However, in both species, the transcripts are distributed exclusively in cells throughout the parotid gland intercalated duct system of adults rather than in acinar cells.…”

Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

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Salivary Glands: A Paradigm for Diversity of Gland Development

Denny

Ball

Redman

1997

Critical Reviews in Oral Biology & Medicine

179

171

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The major salivary glands of mammals are represented by three pairs of organs that cooperate functionally to produce saliva for the oral cavity. While each type of gland produces a signature secretion that complements the secretions from the other glands, there is also redundancy as evidenced by secretion of functionally similar and, in some cases, identical products in the three glands. This, along with their common late initiation of development, in fetal terms, their similarities in developmental pattern, and their proximate sites of origin, suggests that a common regulatory cascade may have been shared until shortly before the onset of overt gland development. Furthermore, occasional ectopic differentiation of individual mature secretory cells in the wrong" gland suggests that control mechanisms responsible for the distinctive cellular composition of each gland also share many common steps, with only minor differences providing the impetus for diversification. To begin to address this area, we examine here the origins of the salivary glands by reviewing the expression patterns of several genes with known morphogenetic potential that may be involved based on developmental timing and location. The possibility that factors leading to determination of the sites of mammalian salivary gland development might be homologous to the regulatory cascade leading to salivarv gland formation in Drosophila is also evaluated. In a subsequent section, cellular phenotypes of neonatal and adult glands are compared and evaluated for insights into the mechanisms and lineages leading to cellular diversification. Finally, the phenomena of proliferation, repair, and regeneration in adult salivary glands are reviewed, with emphasis on the extent to which the cellular diversity is reversible and which cell type other than stem cells has the ability to redifferentiate into other cell types.

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Section: (6) Origin Of the Three Major Types Of Salivary Glandsmentioning

confidence: 99%

Section: (6) Origin Of the Three Major Types Of Salivary Glandsmentioning

confidence: 99%

Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

Section: (2) Perinatal and Adult Protein Phenotypesmentioning

confidence: 99%

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Salivary Glands: A Paradigm for Diversity of Gland Development

Denny

Ball

Redman

1997

Critical Reviews in Oral Biology & Medicine

179

171

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“…For electron microscopic immunogold labeling, tissue (ϳ1 mm 3 ) was fixed 1 h in ice-cold 1% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4, and embedded in LR Gold resin. Thin sections were collected on Formvar-coated nickel grids, then incubated overnight with primary antibody or nonimmune serum.…”

Section: Methodsmentioning

confidence: 99%

Thesldmutation is specific for sublingual salivary mucous cells and disrupts apomucin gene expression

Fallon

Latchney

Hand³

et al. 2003

Physiological Genomics

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NFS/N- sld mice harbor a spontaneous autosomal recessive mutation, sld (sublingual gland differentiation arrest) and histologically display attenuated mucous cell expression in sublingual glands (Hayashi et al. Am J Pathol 132: 187–191, 1988). Because altered serous demilune cell expression is unknown, we determined the phenotypic expression of this cell type in mutants. Moreover, we evaluated whether absence of glycoconjugate staining in 3-day-old mutant glands is related to disruption in apomucin gene expression and/or to posttranslational glycosylation events. Serous cell differentiation is unaffected, determined morphologically and by serous cell marker expression (PSP, parotid secretory protein; and Dcpp, demilune cell and parotid protein). Conversely, apical granules in “atypical” exocrine cells of mutant glands are PSP and mucin negative, but contain abundant SMGD (mucous granule marker). Age-related appearance of mucous cells is associated with expression of apomucin gene products, whereas SMGD expression is unaltered. “Atypical” cells thus appear specified to a mucous cell fate but do not synthesize mucin glycoproteins unless selectively induced postnatally, indicating the sld mutation disrupts apomucin transcriptional regulation and/or decreases apomucin mRNA stability.

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