cDNA sequence and structure of a gene encoding trout testis high‐mobility‐group‐1 protein

Štros, Michal; Nishikawa, Sandra; Dixon, Gordon H.

doi:10.1111/j.1432-1033.1994.00581.x

Cited by 16 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytoplasmic extracts from HeLa cells, uninfected or infected with adenovirus, were prepared as described (24,25 (26) and by perchloric acid extraction (27,28). A mixture of equal amounts of the two extracts was used in the integration assay.…”

Section: Methodsmentioning

confidence: 99%

Adeno-associated virus (AAV) site-specific integration: Formation of AAV– AAVS1 junctions in an in vitro system

Dyall

Szabo

Berns

1999

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

An in vitro system to study the mechanism of site-specific integration of adeno-associated virus (AAV) was developed. This system is based on two substrates, a linear or circular AAV donor and a circular acceptor containing the preintegration locus AAVS1. In the presence of HeLa extract and the His-Tag-purified Rep68 protein, specific covalent junctions between AAV and AAVS1 were formed and detected by PCR. The majority of the junctions were located within the Rep binding site of both the AAV and the AAVS1 substrates, underlining the involvement of the Rep protein. A limited amount of replication and the presence of nuclear factors promoted the efficiency of the reaction. The process was ATPdependent, indicating that the helicase activity of Rep may be important in the formation of the junctions. According to current models of integration, the formation of the junctions would represent a first step in the process of AAV integration. This step could be crucial for the site specificity of the recombination event that leads to the integration of AAV into human chromosome 19 in vivo.

show abstract

Section: Methodsmentioning

confidence: 99%

Adeno-associated virus (AAV) site-specific integration: Formation of AAV– AAVS1 junctions in an in vitro system

Dyall

Szabo

Berns

1999

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Specific gene fragments were amplified from extracted genomic DNA using the polymerase chain reaction (PCR). The genes surveyed in this study were growth hormone II (GHII, Devlin 1993), prolactin II (PRLII, Xiong et al 1992), p53 (Kusser et al 1994), somatolactin (SL, Takayama et al 1991), cytochrome p450A (CYPIA1, Berndtson and Chen 1994), and high mobility group protein (HMG, Stros et al 1994). Primers for the GHII, PRLII, and p53 fragments were from Park et a!.…”

Section: Methods Of Surveying Variationmentioning

confidence: 99%

TESTING MODELS OF MIGRATION AND ISOLATION AMONG POPULATIONS OF CHINOOK SALMON (ONCORHYNCHUS TSCHAWYTSCHA)

Ford

1998

Evolution

View full text Add to dashboard Cite

The chinook salmon (Oncorhynchus tschawytscha) is a behaviorally, morphologically, and ecologically variable species distributed over a large geographic range. Although previous genetic surveys have revealed considerable genetic differences among populations with different life history types and from different major river drainages, it is not clear to what degree these genetically distinct populations are connected by low levels of gene flow. The work described in this paper addresses this question by surveying DNA restriction site variation at six nuclear genes from nine populations encompassing most of the species's North American range, and then attempting to fit the patterns of variation observed at these genes to five different evolutionary models using computer simulations of the coalescent process. Two commonly used constant population size models, one hypothesizing no migration among populations and one hypothesizing equal rates of migration among populations, were found to be statistically inconsistent with the observed patterns of variation. The other three models, which involved either recent divergence among populations coupled with large changes in populations size, unequal migration rates among populations, or selection, were all found to be consistent with the observed patterns of variation. Assuming selective neutrality, these results suggest that either the populations have all descended from a common ancestral population within the last ~50,000 years and have all suffered large declines in effective population size since that time, or that they have a more ancient divergence time but are connected by low levels of gene flow. These conclusions rest on the assumption of selective neutrality. With the methods employed, it was not possible to simultaneously test hypotheses of both selective neutrality and population structure.

show abstract

“…The PCR primers contained restriction sites for BamHI and SalI, as well as the initiation and termination codons (underlined). The PCR protocol was as described previously (34) with the following modification of the PCR cycles: 94°C for 4 min (denaturation cycle); 94°C for 40 s, 60°C for 20 s, 72°C for 40 s (30 cycles); and 72°C for 7 min (extension cycle). The amplified HMG box domain was purified on a 1% agarose gel, blunt-ended by T4 DNA polymerase and cloned into the SmaI site of the plasmid pAlter-1 (Promega).…”

Section: Plasmidsmentioning

confidence: 99%

A Role of Basic Residues and the Putative Intercalating Phenylalanine of the HMG-1 Box B in DNA Supercoiling and Binding to Four-way DNA Junctions

Štros

Muselı́ková

2000

Journal of Biological Chemistry

View full text Add to dashboard Cite

103 of helix I is important for DNA supercoiling but dispensable for binding to supercoiled DNA and 4WJs. We conclude that the B domain of HMG-1 can tolerate substitutions of a number of amino acid residues without abolishing the structurespecific recognition of 4WJs, whereas mutations of most of these residues severely impair the topoisomerase Imediated DNA supercoiling and change the sign of supercoiling from negative to positive.

show abstract

cDNA sequence and structure of a gene encoding trout testis high‐mobility‐group‐1 protein

Cited by 16 publications

References 44 publications

Adeno-associated virus (AAV) site-specific integration: Formation of AAV– AAVS1 junctions in an in vitro system

Adeno-associated virus (AAV) site-specific integration: Formation of AAV– AAVS1 junctions in an in vitro system

TESTING MODELS OF MIGRATION AND ISOLATION AMONG POPULATIONS OF CHINOOK SALMON (ONCORHYNCHUS TSCHAWYTSCHA)

A Role of Basic Residues and the Putative Intercalating Phenylalanine of the HMG-1 Box B in DNA Supercoiling and Binding to Four-way DNA Junctions

Contact Info

Product

Resources

About