CE at the omics level: Towards systems biology – An update

Abstract

This review provides an updated overview of recent developments and applications of CE based on previously published reports in the field of omic research. The increased number of published articles on omics shows that the field is growing and attracting the attention of many life science researchers. Due to developments in the omics sciences, many researchers have been studying systems biology, in which biological events in organisms are systematically interpreted through the combination of complex measurements from various methods resulting in high-throughput data. Given the challenges of such complex forms of analysis, CE is a strong candidate for generating omics data useful for acquiring the qualitative and quantitative knowledge necessary for systems-level investigation. By emphasizing CE for systems biology, this review will discuss and focus on the applicability of CE to systems-based analytical data at the genomic, transcriptomic, proteomic, and metabolomic levels from 2005 to the present.

Capillary electrophoresis of some free fatty acids using partially aqueous electrolyte systems and indirect UV detection. Application to the analysis of oleic and linoleic acids in peanut breeding lines

“…In addition, CE is a versatile separation technique that is increasingly being employed in the analysis of complex natural matrices, e.g., food (for a review see ref. [12]) and systems biology [13] due to the fact that CE offers high separation efficiency and unique selectivity.…”

Protein attachment onto silica surfaces – a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE

“…Although unnoticed by the public, the successful application of CE in deciphering the human genome and its responsibility for the premature accomplishment of the human genome project [2] can be considered one of the milestones in establishing this technique within the portfolio of biochemical applications. Due to its unique properties, CE becomes increasingly implemented in the ''omics'' field of research [3,4], including, e.g. genomics [5,6], metabolomics [7][8][9][10], glycomics [11][12][13] and particularly proteomics [14,15].…”

Multi‐wavelength light emitting diode array as an excitation source for light emitting diode‐induced fluorescence detection in capillary electrophoresis

“…CE is important in the area of genomics, transcriptomics, proteomics, metabolomics and pharmaceutics [1], and while UV/Vis absorbance detection is by far the most common method of detection, it is not as sensitive as fluorescence detection. Fluorescence detection is typically performed using a single excitation source, with the most common being the 488 nm line from an argon ion laser.…”