CE determination of quinolones in the presence of bovine serum albumin

Abstract

This article describes the influence of bovine serum albumin (BSA) as an additive on the capillary electrophoresis-potential gradient determination of five quinolones, enoxacin, norfloxacin, ofloxacin, fleroxacin, and pazufloxacin. With 10 mg/L of BSA present in the buffer of 30 mM Tris and 3 mM phosphoric acid at pH 9, the detection limits of the five quinolones were in the range of 0.24-0.68 mg/L, i. e. 5.8-16.5-fold lower than those obtained with the buffer devoid of BSA, and the analysis time was shortened. We suggest that the inner wall-adsorbed BSA suppresses the adsorption of quinolones and simultaneously enhances the electroosmotic flow rate. Our experiments indicated that adopting the potential gradient detection technique could eliminate the interference of the UV-active proteins on the detection of quinolones that would occur with conventional optical detection, and therefore offer high detection sensitivity. As a demonstration, the method was applied to the determination of QNs in fortified chicken muscle sample with satisfactory results.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…Consequently, a number of novel CE developments are aimed at improving sensitivity in the detection of these substances by different means. For instance, the analysis of fluoroquinolones (FQs) has been investigated using different detection schemes, sample preconcentration and stacking methods 104–110. Recently, the detection of five quinolones, namely, enoxacin, norfloxacin, ofloxacin, fleroxacin and pazufloxacin has been approached by CE using potential gradient detection (PGD) in fortified milk samples 104.…”

Analytical Methods for Determining Third and Fourth Generation Fluoroquinolones: A Review

“…The methods for determination of PAZU comprise both HPLC [ 26 , 27 , 49 ] and CE technique [ 50 – 52 ]. The analysed matrices were pharmaceutical formulation (tablets), milk [ 50 ], and biological fluids, i.e., serum, plasma, urine, muscle homogenate, saliva, gingiva, and crevicular fluid [ 26 , 27 , 49 , 51 , 52 ]. The applied columns in HPLC were C18 [ 27 , 49 ] and C8 [ 26 ].…”

“…The other contents were 0.5% phosphoric acid containing 1% of TEA [ 49 ] or 1% TEA solution [ 26 ] or 0.1% formic acid at pH 3.0 adjusted with TEA [ 27 ]. CE separation was performed at room temperature, and the background electrolyte (BGE) was an aqueous solution of TRIS and phosphoric acid [ 50 , 51 ]. The other additives were phosphates and β-cyclodextrines [ 52 ].…”

“…A lower voltage was applied and a shorter capillary was used (Table 1 ). The sample preparation involved protein precipitation with methanol [ 49 ], ACN [ 27 ] or 6% perchloric acid and methanol [ 26 ], LLE with dichloromethane [ 50 , 51 ], and dilution [ 52 ]. The LOD depended on a sample preparation—for extraction it was within the range 0.01–0.3 μg mL −1 [ 49 – 51 ], for protein precipitation 0.01–0.1 μg mL −1 [ 26 , 27 ], and for diluted urine it was the highest at 7 μg mL −1 [ 52 ] (Table 1 ).…”

Recent advances in the analysis of antibiotics by CE and CEC

“…The authors suggested that the presence of BSA suppressed the adsorption of quinolones in the inner wall of the capillary and increased the EOF rate. Finally, the method was applied to the determination of fluoroquinolones in spiked samples obtaining recoveries in the range from 81.9 to 104.3% 37.…”