CE‐MS for metabolomics: Developments and applications in the period 2010–2012

Ramautar, Rawi; Somsen, Govert W.; Jong, Gerhardus J. de

doi:10.1002/elps.201200390

Cited by 109 publications

(65 citation statements)

References 64 publications

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“…NMR alone can detect data on hundreds of metabolite components in biological samples, and does not require the properities of materials to be detected in advance. Meanwhile, NMR requires less sample, does not require complicated sample preparation or derivatives, and the sample can be recovered; thus, NMR is currently one of the most common research methods used in metabonomics (11). Given each sample's original data matrix, standardization and other preprocessing methods, all detected data can be summed to enable metabonomics data to be used in multivariate statistic analysis (12).…”

Section: Metabonomics Research Methodsmentioning

confidence: 99%

Research methods of metabonomics and its applications in acupuncture

Junmei¹,

Xuan²

2015

Curr Res Integr Med

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Metabonomics is a new 'omic' that has emerged in the postgenomic era. Recently, metabonomics technology is increasingly and widely used in the field of traditional Chinese medicine research; however, metabonomics research investigating the function and mechanisms of acupuncture is still at an exploratory stage. The present article describes the unique advantages of metabonomics in systems biology, analyzes the application of metabonomics, and presents a preliminary exploration of the application of metabonomics in modern acupuncture research including sham acupuncture, time acupuncture, mechanistic research investigating the effects of acupuncture on serious illness and the compatibility of prescriptions with acupuncture in clinical research. etabonomics is a new 'omic' that has emerged in the postgenomic era. It has the advantages of high throughput, sensitivity and accuracy. To date, the application of metabonomics technology in research has been preliminary, and there is considerable potential for its application in research investigating traditional Chinese medicine. Both acupuncture and metabonomics technology are superior techniques, but metabonomics research related to the function and mechanism of acupuncture is still in the exploratory stage, and there has been relatively little research in this field. Applying metabonomics to studying acupuncture will help to elucidate the mechanism of acupuncture thoroughly and systematically, and will promote acupuncture theory and clinical development. The present article describes both the definition and research methods of metabonomics, as well as its potential application in acupuncture. DEfInITIOn Of METABOnOMICSNicholson et al (1) first proposed the definition of metabonomics in 1999. 'Metabonomics' refers to the quantitative analysis of all metabolites in a biological system. Fiehn (2), Nielsen and Oliver (3), and other authors have proposed different but related definitions of metabonomics, which include: metabolic profiling analysis (quantitative determination of specific predetermined structure-and property-related metabolites); metabolite target analysis (quantitative determination of products or substrates of a target protein); and metabolite fingerprinting (qualitatively and semiquantitatively analyzing internal and external metabolites of cells).Metabonomics research is based on the analysis of metabolic group indexes and uses high-throughput tests and different data processing techniques and targets to model biological information, which is collected and systematically meta-analyzed. It represents an important branch of systems biology, and is another research field and branch of systems biology after genomics, proteomics and transcriptomics. It is a research method that quantitatively analyzes a variety of time-related responses of metabolic parameters when an organism is affected by exogenous stimuli or genetic modification (4). Metabonomics studies the change of all small molecule metabolites with molecular weight <1000 Da when the organism is subje...

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Section: Metabonomics Research Methodsmentioning

confidence: 99%

Research methods of metabonomics and its applications in acupuncture

Junmei¹,

Xuan²

2015

Curr Res Integr Med

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“…LC-MS can detect a relatively broad spectrum of (polar and non-polar) metabolites with ample selectivity and sensitivity [30] . CE-MS is a rather new technique in metabolomics that is more sensitive and detects a wider spectrum of (polar) compounds than LC-MS [31,32] . 1 H-NMR is a non-destructive technique that does not require prior separation of the compounds in the biofluid.…”

Section: Analytical Strategiesmentioning

confidence: 99%

Metabolomics as a diagnostic tool in gastroenterology

Preter¹,

Verbeke²

2013

WJGPT

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Metabolomics has increasingly been applied in addition to other "omic" approaches in the study of the pathophysiology of different gastrointestinal diseases. Metabolites represent molecular readouts of the cell status reflecting a physiological phenotype. In addition, changes in metabolite concentrations induced by exogenous factors such as environmental and dietary factors which do not affect the genome, are taken into account. Metabolic reactions initiated by the host or gut microbiota can lead to "marker" metabolites present in different biological fluids that allow differentiation between health and disease. Several lines of evidence implicated the involvement of intestinal microbiota in the pathogenesis of inflammatory bowel disease (IBD). Also in irritable bowel syndrome (IBS), a role of an abnormal microbiota composition, so-called dysbiosis, is supported by experimental data. These compositional alterations could play a role in the aetiology of both diseases by altering the metabolic activities of the gut bacteria. Several studies have applied a metabolomic approach to identify these metabolite signatures. However, before translating a potential metabolite biomarker into clinical use, additional validation studies are required. This review summarizes contributions that metabolomics has made in IBD and IBS and presents potential future directions within the field.

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“…CE-MS can be applied in fields such as proteomics, metabolomics, pharmaceutical, forensic science and food analysis. For example it is used in the analysis of biomarker in the body fluids (urine, CSF, plasma) in disease diagnosis [86][87][88].…”

Section: Ace-msmentioning

confidence: 99%

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

et al. 2014

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The present review covers recent advances and important applications of affinity capillary electrophoresis (ACE). It provides an overview about various ACE types, including ACE-MS, the multiple injection mode, the use of microchips and field-amplified sample injection-ACE. The most common scenarios of the studied affinity interactions are protein-drug, protein-metal ion, protein-protein, protein-DNA, protein-carbohydrate, carbohydrate-drug, peptide-peptide, DNA-drug and antigen-antibody. Approaches for the improvements of ACE in term of precision, rinsing protocols and sensitivity are discussed. The combined use of computer simulation programs to support data evaluation is presented. In conclusion, the performance of ACE is compared with other techniques such as equilibrium dialysis, parallel artificial membrane permeability assay, high-performance affinity chromatography as well as surface plasmon resonance, ultraviolet, circular dichroism, nuclear magnetic resonance, Fourier transform infrared, fluorescence, MS and isothermal titration calorimetry.

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CE‐MS for metabolomics: Developments and applications in the period 2010–2012

Cited by 109 publications

References 64 publications

Research methods of metabonomics and its applications in acupuncture

Research methods of metabonomics and its applications in acupuncture

Metabolomics as a diagnostic tool in gastroenterology

Recent Advances in Affinity Capillary Electrophoresis for Binding Studies

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