Cecropin B loaded TiO2 nanotubes coated with hyaluronidase sensitive multilayers for reducing bacterial adhesion

Shen, Xinkun; Zhang, Fei; Li, Ké; Qin, Chenhu; Ma, Pingping; Dai, Liangliang; Cai, Kaiyong

doi:10.1016/j.matdes.2015.12.126

Cited by 46 publications

(18 citation statements)

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“…In recent years, the research on photocatalysis technology of TiO 2 has attracted increasing attention from scientic communities due to its appealing advantages such as the high catalytic oxidation activity, the fast photocatalysis degradation reaction, the high photocatalytic activity, and so on. 2,3 Thus, TiO 2 based photocatalysts have great potential for applications in a wide range of areas, for example, environmental management, 4 development of new energy, [5][6][7][8][9] air purication, 10,11 sewage treatment, 12 self-cleaning ceramics, 13,14 sterilization material, [15][16][17][18] photoelectric conversion, 19,20 etc. However, the application of TiO 2 based photocatalysts is still limited due to the following two aspects.…”

Section: Introductionmentioning

confidence: 99%

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO₂

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO₂

et al. 2019

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“…Meanwhile, there was a characteristic peak of the aromatic ring at 747.67 cm −1 which was consistent with CIP (at 750.44 cm −1 ). Besides, additional peak at 1655.94 and 1632.13 cm −1 were observed, which were contributed to the condensation reaction between the carboxyl of CIP and the amino group of OGP …”

Section: Resultsmentioning

confidence: 98%

“…Osteoblasts were isolated from neonatal rat skull as described previously . All Ti disks (sterilized by ultraviolet) were placed in a 24‐well plate for cell seeding and culturing.…”

Section: Methodsmentioning

confidence: 99%

“…Cell seed and culture Osteoblasts were isolated from neonatal rat skull as described previously. 46 All Ti disks (sterilized by ultraviolet) were placed in a 24-well plate for cell seeding and culturing. Cells were seeded onto bare Ti, Ti-OGP, and Ti-OGP-CIP substrates with an initial cell seeding density of 1 × 10 4 cells/cm 2 for all experiments (except for specified description) and cultured with DMEM (high glucose) supplementing with 10% FBS at 37 C under 5% CO 2 atmosphere.…”

Section: Bacteria Morphology Observationmentioning

confidence: 99%

“…Besides, additional peak at 1655.94 and 1632.13 cm −1 were observed, which were contributed to the condensation reaction between the carboxyl of CIP and the amino group of OGP. 46 Additionally, the 1 H NMR spectrums of OGP, CIP, and OGP-CIP were presented in Figure 3 (referenced to residual H 2 O peak at 4.79 ppm). Comparing with pure OGP, some feature peaks of δ 3.54, 3.64, and 8.68 were observed in the spectrum of OGP-CIP, which were ascribed to H a , H b , and H c (referenced to the spectrum of CIP 43 ).…”

Section: Sample Fabrication and Characterizationmentioning

confidence: 99%

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Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

Liu

Yang

Tao

et al. 2018

J Biomedical Materials Res

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The inherent bioinertness and potential bacterial infection risk are the two leading causes for Ti implant failure. To improve osseointegration and antibiosis, in this work, a novel antimicrobial osteogenic growth peptide was first synthesized by conjugating osteogenic growth peptide (OGP) and ciprofloxacin (CIP). Then, the synthetic antimicrobial peptide was immobilized onto Ti implant surface for chemoselective binding via the amide reaction. Thereafter, the capabilities of modified Ti implant on osseointegration and antibiosis were measured with cell experiments and antimicrobial activity in vitro. The results showed that antimicrobial osteogenic growth peptide (OGP-CIP) was successfully prepared and grafted onto Ti implant surface. Moreover, the antimicrobial peptide-modified Ti implants could promote osteoblasts spreading and osteodifferentiation compared with unmodified Ti substrates. Meanwhile, in vitro bacteria studies (Staphylococcus aureus and Escherichia coli) proved that the antibacterial property of antimicrobial peptide functionalized Ti implant was improved obviously. The method used in this work is a feasible and promising strategy to win the race against invading bacteria and accelerate bone integration in orthopedic implantation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 3021-3033, 2018.

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Calcium Peroxide Nanoparticles‐Embedded Coatings on Anti‐Inflammatory TiO₂ Nanotubes for Bacteria Elimination and Inflammatory Environment Amelioration

Yang

Leng

et al. 2021

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materials and therapies for skin wound repair. [1][2][3] Pathogenic bacteria such as Staphylococcus aureus can adhere to the surface of implants to avoid death from the sterilization procedure, or are present in adjacent tissues prior to implantation. [4] If the bacterial contamination cannot be treated and effectively removed from the site, the surviving bacteria will recover and rapidly re-colonize, which may give rise to tissue damage and inflammation. [5,6] Although more and more antibiotics have been approved by FDA to treat malignant bacterial infections and generally exhibit sufficient antibacterial activity, the fast emergence of multi-drug resistant bacteria still reminds us to prevent the excessive use of antibiotics. Other commonly used antibacterial strategies, such as photothermal treatment and sonodynamic therapy, always bring potential side effects on surrounding cells/tissues. Accordingly, the development of an ideal antibacterial component will be a long-term and arduous striving goal.Medical-grade titanium is one of the main types of metal implants used, due to its high biocompatibility and its ability to safely sustain extensive contact with soft tissues such as gum, muscle, and skin. [7][8][9] Currently, titanium implants are treated with antibacterial coatings to avoid potential bacterial infections, [10] such as surface modifications with antibiotics, anti-bacterial metallic Implant-associated bacterial infections significantly impair the integration between titanium and soft tissues. Traditional antibacterial modifications of titanium implants are able to eliminate bacteria, but the resulting proinflammatory reactions are usually ignored, which still poses potential risks to human bodies. Here, a dual drug-loading system on titanium has been developed via the adhesion of a catechol motif-modified methacrylated gelatin hydrogel onto TiO 2 nanotubes. Then synthesized CaO 2 nanoparticles (NPs) are embedded into the hydrogel, and interleukin-4 (IL-4) is loaded into the nanotubes to achieve both antibacterial and anti-inflammatory properties. The dual drug-loading system can eliminate Staphylococcus aureus (S. aureus) rapidly, attributed to the H 2 O 2 release from CaO 2 NPs. The potential cytotoxicity of CaO 2 NPs is also remarkably reduced after being embedded into the hydrogel. More importantly, with the gradual release of IL-4, the dual drug-loading system is capable of modulating pro-inflammatory reactions by inducing M2 phenotype polarization of macrophages. In a subcutaneous infection model, the S. aureus contamination is effectively resolved after 2 days, and the resulting pro-inflammatory reactions are also inhibited after 7 days. Finally, the damaged tissue is significantly recovered. Taken together, the dual drug-loading system exhibits great therapeutic potential in effectively killing pathogens and inhibiting the resulting pro-inflammatory reactions.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.202102907.

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Cecropin B loaded TiO2 nanotubes coated with hyaluronidase sensitive multilayers for reducing bacterial adhesion

Cited by 46 publications

References 57 publications

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO₂

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO₂

Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

Calcium Peroxide Nanoparticles‐Embedded Coatings on Anti‐Inflammatory TiO₂ Nanotubes for Bacteria Elimination and Inflammatory Environment Amelioration

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Cecropin B loaded TiO2 nanotubes coated with hyaluronidase sensitive multilayers for reducing bacterial adhesion

Cited by 46 publications

References 57 publications

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO2

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO2

Preparing and immobilizing antimicrobial osteogenic growth peptide on titanium substrate surface

Calcium Peroxide Nanoparticles‐Embedded Coatings on Anti‐Inflammatory TiO2 Nanotubes for Bacteria Elimination and Inflammatory Environment Amelioration

Contact Info

Product

Resources

About

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO₂

Effect of heat treatment under vacuum on structure and visible-light photocatalytic activity of nano-TiO₂

Calcium Peroxide Nanoparticles‐Embedded Coatings on Anti‐Inflammatory TiO₂ Nanotubes for Bacteria Elimination and Inflammatory Environment Amelioration