Celastrol inhibits colorectal cancer through TGF-&amp;beta;1/Smad signaling

Jiang, Zhitao; Cao, Qianyu; Dai, Guoliang; Wang, Jianchun; Liu, Chundi; Lv, Lingyan; Jin-huo, Pan

doi:10.2147/ott.s187817

Cited by 39 publications

(17 citation statements)

References 23 publications

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“…Furthermore, when compared with the control group, miR-590-5p inhibitor decreased cell migration and invasion in vitro, which was consistent with the results obtained in a previous study (17). Of note, miR-590-5p inhibitor suppressed the TGF-β/Smad2/3 signaling pathway, which regulates CRC cell viability and migration (23,25,26). Collectively, the results indicated that miR-590-5p knockdown inhibited the TGF-β/Smad2/3 signaling pathway in HCT116 and SW480 cells.…”

Section: Discussionsupporting

confidence: 90%

miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4

et al. 2020

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Recent studies have revealed that microRNAs (miRs) are involved in the pathogenesis of colorectal cancer (CRC); however, the roles of miR-590-5p in CRC are not completely understood. Therefore, the present study investigated the expression of miR-590-5p and programmed cell death 4 (PDCD4) in CRC tissues and healthy adjacent tissues via reverse transcription-quantitative PCR. Furthermore, human CRC cells were cultured in vitro and transfected with miR-590-5p inhibitor. CRC cell viability, migration and invasion were evaluated by conducting MTT, wound healing and Transwell assays, respectively. Additionally, the relative expression of PDCD4 and phosphorylated-Smad2/3 was analyzed via western blotting. miR-590-5p was significantly upregulated and PDCD4 was significantly downregulated in CRC tissues compared with healthy adjacent tissues. Moreover, the results indicated that miR-590-5p knockdown inhibited cell viability and migration by altering the expression of PDCD4, transforming growth factor-β and phosphorylated-Smad2/3. PDCD4 was identified as a direct target of miR-590-5p. In conclusion, the results of the present study suggested that miR-590-5p may regulate CRC cell viability and migration, indicating that miR-590-5p may serve as a potential therapeutic target for CRC.

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Section: Discussionsupporting

confidence: 90%

miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4

et al. 2020

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“…TGF-β inhibits normal intestinal epithelium cell proliferation and induces apoptosis and differentiation. Therefore, TGF-β acts as a tumor suppressor in the normal intestinal epithelium [33]. Many CRCs loose tumor suppressor proteins such as TGF-β in the initiation and further stages [28].…”

Section: Tgf-β Signaling Pathway In Crcmentioning

confidence: 99%

Signaling pathways involved in colorectal cancer progression

et al. 2019

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Colorectal cancer (CRC) is the fourth leading cause of the worldwide cancer mortality. Different molecular mechanisms have been attributed to the development and progress of CRC. In this review, we will focus on the mitogen-activated protein kinase (MAPK) cascades downstream of the epidermal growth factor receptor (EGFR), Notch, PI3K/AKT pathway, transforming growth factor-β (TGF-β), and Wnt signaling pathways. Various mutations in the components of these signaling pathways have been linked to the development of CRC. Accordingly, numerous efforts have been carried out to target the signaling pathways to develop novel therapeutic approaches. Herein, we review the signaling pathways involved in the incidence and progression of CRC, and the strategies for the therapy targeting components of signaling pathways in CRC.

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“…Then we analyzed their enrichment in GO and signaling pathways and found that the DEGs were enriched in 11 pathways, including the PI3K-Akt signaling pathway. Previous studies showed that several signal pathways could be involved in the progress and development of CRC [18][19][20][21][22][23]. PI3K/AKT is one of the classic pathways responsible for multiple biological processes, such as cell proliferation, differentiation, and migration.…”

Section: Discussionmentioning

confidence: 99%

Identification and verification of 3 key genes associated with survival and prognosis of patients with colon adenocarcinoma via integrated bioinformatics analysis

Liu¹,

Li²,

Dong

et al. 2020

Preprint

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Background: Colorectal cancer (CRC) is the third most lethal malignancy in the world, wherein colon adenocarcinoma (COAD) is the most prevalent type of CRC.Exploring biomarkers is important for the diagnosis, treatment, and prevention of COAD. Methods: We used GEO2R and Venn online software for differential gene screening analysis. Hub genes were screened via STRING and Cytoscape, following Gene Ontology and KEGG enrichment analysis. Finally, survival analysis and expression validation were performed via UALCAN online software, real-time PCR and immunohistochemistry. Results: In this study, we screened 323 common differentially expressed genes from four GSE datasets. Furthermore, four hub genes were selected for survival correlation analysis and expression level verification, three of which were shown to be statistically significant. Conclusion: Our study suggests that SERPINE1, SPP1 and TIMP1 may be biomarkers closely related to the prognosis of CRC patients.

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Celastrol inhibits colorectal cancer through TGF-β1/Smad signaling

Cited by 39 publications

References 23 publications

miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4

miR‑590‑5p may regulate colorectal cancer cell viability and migration by targeting PDCD4

Signaling pathways involved in colorectal cancer progression

Identification and verification of 3 key genes associated with survival and prognosis of patients with colon adenocarcinoma via integrated bioinformatics analysis

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