Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide

Hwang, Dong Soo; Sim, Sung Bo; Joon, Hyung

doi:10.1016/j.biomaterials.2007.05.028

Cited by 188 publications

(159 citation statements)

References 30 publications

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“…As LIPUS has the potential to act as a physical force on cells (Argadine et al, 2005;Padilla et al, 2014), and FAs are known to be involved in mechanosensing (Geiger et al, 2009;Shemesh et al, 2005), we tested the possibility that FAs are involved in LIPUS sensing. B16 GFP-actin cells were seeded for 1 h on either fibronectin (an integrin ligand) or Cell-Tak (a substrate to which cells attach and spread in an integrinindependent manner) (Hwang et al, 2007). B16 GFP-actin cells on fibronectin, but not Cell-Tak, developed large FAs associated with actin stress fibres (Fig.…”

Section: Lipus Induces the Rapid Activation Of The Rho Gtpases Rac1 Amentioning

confidence: 99%

Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity

Atherton

Lausecker

Harrison³

et al. 2017

Journal of Cell Science

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Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates that the mechanosensitive focal adhesion (FA) protein vinculin, and both focal adhesion kinase (FAK, also known as PTK2) and Rab5 (both the Rab5a and Rab5b isoforms) have key roles in regulating these effects. Inhibiting the link of vinculin to the actin-cytoskeleton abolished LIPUS sensing. We show that this vinculin-mediated link was not only critical for Rac1 induction and actin rearrangements, but was also important for the induction of a Rab5-dependent increase in the number of early endosomes. Expression of dominant-negative Rab5, or inhibition of endocytosis with dynasore, also blocked LIPUSinduced Rac1 signalling events. Taken together, our data show that LIPUS is sensed by cell matrix adhesions through vinculin, which in turn modulates a Rab5-Rac1 pathway to control ultrasound-mediated endocytosis and cell motility. Finally, we demonstrate that a similar FAK-Rab5-Rac1 pathway acts to control cell spreading upon fibronectin.

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Section: Lipus Induces the Rapid Activation Of The Rho Gtpases Rac1 Amentioning

confidence: 99%

Low-intensity pulsed ultrasound promotes cell motility through vinculin-controlled Rac1 GTPase activity

Atherton

Lausecker

Harrison³

et al. 2017

Journal of Cell Science

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“…However, very small arterioles may also be studied without pressure and flow, being positioned at the bottom of a recording chamber using suction micropipettes or bioadhesive proteins. 24 Measurements of local and conducted diameters require a light microscope equipped with a digital or video camera for recording of the experiments. The local responses are elicited by placing the tip of a micropipette (tip 1 to 5 mm) adjacent to an arteriole for application of agonists such as norepinephrine (NE), phenylephrine (PE), ACh, or KCl, etc.…”

Section: How Vascular Conducted Responses Are Typically Measuredmentioning

confidence: 99%

The Vascular Conducted Response in Cerebral Blood Flow Regulation

Jensen¹,

Holstein‐Rathlou

2013

J Cereb Blood Flow Metab

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Despite recent advances in our understanding of the molecular and cellular mechanisms behind vascular conducted responses (VCRs) in systemic arterioles, we still know very little about their potential physiological and pathophysiological role in brain penetrating arterioles controlling blood flow to the deeper areas of the brain. The scope of the present review is to present an overview of the conceptual, mechanistic, and physiological role of VCRs in resistance vessels, and to discuss in detail the recent advances in our knowledge of VCRs in brain arterioles controlling cerebral blood flow. We provide a schematic view of the ion channels and intercellular communication pathways necessary for conduction of an electrical and mechanical response in the arteriolar wall, and discuss the local signaling mechanisms and cellular pathway involved in the responses to different local stimuli and in different vascular beds. Physiological modulation of VCRs, which is a rather new finding in this field, is discussed in the light of changes in plasma membrane ion channel conductance as a function of health status or disease. Finally, we discuss the possible role of VCRs in cerebrovascular function and disease as well as suggest future directions for studying VCRs in the cerebral circulation.

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“…The Laf_CBM-rev primer introduced a RGD motif followed by a hexahistidine tag at the Cterminus of the CBM. The RGD motif is found in the extracellular matrix and blood proteins, such as fibronectin, vitronectin, osteopontin, collagens, thrombospondin, fibrinogen, and von Willebrand factor and has been described as the major functional group responsible for cellular adhesion (Hwang et al, 2007;Ruoslahti & Pierschbacher, 1987). The inclusion of a RGD motif at the C-terminus of the laforin CBM was intended to be a cell attachment motif in starch-based biomaterial functionalized with the CBM-RGD protein .…”

Section: Heterologous Protein Expression and Purificationmentioning

confidence: 99%