Cell-based assays for profiling activity and safety properties of cancer drugs

Li, Weiwei; Lam, Marilyn; Birkeland, Andrew C.; Riffel, Angela; Montana, Leticia; Sullivan, M.; Post, Joseph

doi:10.1016/j.vascn.2006.02.014

Cited by 42 publications

(26 citation statements)

References 18 publications

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“…Numerous reports have indicated that both the microtubule stabilizing agents (like Taxol) and the microtubule depolymerizing agents (like vincristine, vinblastine, and colchicine) (Checchi et al, 2003;Honore et al, 2005;Li et al, 2006) show strong anticancer activity through inhibiting the cell cycle progression and inducing programmed cell death (Li et al, 2006). Therefore our study clearly shows that BFE at mild concentrations (50 and 30 µg/mL) has cytotoxic effects on bladder cancer cells as well as inhibition of the cell growth through cell cycle arrest.…”

Section: Discussionsupporting

confidence: 56%

Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Roudsari

Bahrami²,

Dehghani³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations (200 µg/mL) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and 30 µg/mL) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.

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Section: Discussionsupporting

confidence: 56%

Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Roudsari

Bahrami²,

Dehghani³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…In addition to cardiotoxicity, another significant side effect of Adriamycin is immunosuppression, specifically bone marrow depression and macrophage dysfunction [26,27]. Bone marrow is one of the critical tissues for the generation of immune cells while activation of macrophages is one of the first steps involved in the initiation of an immune response.…”

Section: Discussionmentioning

confidence: 99%

Influence of the phosphodiesterase-5 inhibitor, sildenafil, on sensitivity to chemotherapy in breast tumor cells

Gennings

Bear

et al. 2010

Breast Cancer Res Treat

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Studies were performed to determine the influence of the phosphodiesterase-5 inhibitor, sildenafil, on sensitivity to adriamycin (doxorubicin) in four human breast tumor cell lines and one murine breast tumor line. Sildenafil did not interfere with the effectiveness of adriamycin in any of the cell lines tested. Sildenafil also failed to protect MDA-MB231 cells against the cytotoxicity of cisplatin, taxol or camptothecin. Sildenafil enhanced sensitivity to adriamycin markedly in the p53 mutant MDA-MB231 and p53 null MCF-7/E6 cells and moderately in the MCF-7/caspase 3 and 4T1 cell lines. In the MDA-MB231 cells, sildenafil increased the extent of DNA damage induced by adriamycin as well as the extent of apoptotic cell death. Sildenafil did not influence sensitivity to adriamycin in bone marrow cells or macrophages. In an immunocompetent model of breast cancer (4T1 mammary carcinoma in Balb/c mice), sildenafil did not attenuate the antitumor effects of adriamycin; furthermore, the combination of sildenafil with adriamycin was no more toxic to the animals than adriamycin alone. Given that sildenafil has been shown to have the potential to protect the heart against the toxicity of adriamycin, these studies suggest that the inclusion of sildenafil with conventional chemotherapeutic protocols involving adriamycin (and possibly cisplatin, camptothecin and/or paclitaxel) should not compromise the antitumor effectiveness of these drugs nor enhance their toxicity to the patient.

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“…Toxicity is a major reason that causes drug failure in the development stage. Primary cells isolated from tissues possess many properties of normal cells in vivo, and they are suitable systems for assessing tissue-specific toxicity (Li et al 2006). In this perspective, human PBMC is an interesting system to evaluate toxicity in vitro (Das Sarma et al 2007;Kundakoviae et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Effect of metronidazole analogues on Giardia lamblia cultures

et al. 2007

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We comparatively evaluate the effect of metronidazole (MTZ) and its five analogues on trophozoites of Giardia lamblia axenically growing. The compounds MTZ-Ms, MTZ-I, MTZ-Br, MTZ-N(3), and MTZ-NH(3)Cl were obtained by molecular modification of the side chain of MTZ. Four of them presented higher giardicidal activity when compared with MTZ. Among them, MTZ-Br and MTZ-I were the most active, without cytotoxic effects against mitogen-activated human peripheral blood mononuclear cells (PBMC). The alteration of MTZ side chain constitutes a fruitful field to develop new drugs for the treatment not only of giardiasis but also of other diseases and signalize that metronidazole analogues are promising candidates as giardicidal and should be further evaluated.

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Cell-based assays for profiling activity and safety properties of cancer drugs

Cited by 42 publications

References 18 publications

Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Bracken-fern Extracts Induce Cell Cycle Arrest and Apoptosis in Certain Cancer Cell Lines

Influence of the phosphodiesterase-5 inhibitor, sildenafil, on sensitivity to chemotherapy in breast tumor cells

Effect of metronidazole analogues on Giardia lamblia cultures

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