Cell block technique as an additional tool in the diagnosis of ameloblastoma

Belatto, Maria Fernanda da Silva; Ramos, Grasieli de Oliveira; Martins, Manoela Domingues; Grando, Liliane Janete; Rivero, Elena Riet Correa

doi:10.1590/1807-3107bor-2014.vol28.0044

Cited by 7 publications

(17 citation statements)

References 14 publications

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“…In addition, only one study with a small sample size assessed the diagnostic capability of cell block for ameloblastoma and found a concordance rate of 50% . Other investigators reported that the diagnosis of ameloblastoma of the jaws by cell block is challenging due to its unspecific cytological characteristics; however, this technique may be useful for the differential diagnosis with inflammatory cysts or keratocysts, based on the absence of cholesterol crystal clefts or keratin in ameloblastoma cases …”

Section: Discussionmentioning

confidence: 99%

“…11 Other investigators reported that the diagnosis of ameloblastoma of the jaws by cell block is challenging due to its unspecific cytological characteristics; however, this technique may be useful for the differential diagnosis with inflammatory cysts or keratocysts, based on the absence of cholesterol crystal clefts or keratin in ameloblastoma cases. 31 Regarding odontogenic cysts, FNAC smear technique concordance rates with histopathology were considerably high. It is important to mention that some lesions present features that facilitate the cytological diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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Diagnostic capability of fine‐needle aspiration cytology in assessment of intraosseous lesions of the jaws: A systematic review

Pilati

Santos

Melo

et al. 2020

Diagnostic Cytopathology

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Purpose To systematically review (SR) and critically appraise studies that investigated the diagnostic capability of fine‐needle aspiration cytology (FNAC) in the assessment of intraosseous lesions of the jaws. Methods Six main electronic databases and three gray literature databases were searched aiming diagnostic studies, which were selected in a two‐phase process. Risk of bias (RoB) of included studies was assessed using the Revised Tool for the Quality Assessment of Diagnostic Accuracy Studies. Results A total of eight articles were included, of which six evaluated the FNAC smear technique exclusively, one assessed both smear and cell‐block techniques, while one investigated only the cell‐block technique. With regard to the FNAC smear technique, from 134 benign lesions investigated, concordance with the histopathological diagnosis was achieved in 64.17% of cases. In addition, considering the 32 malignant lesions assessed through this technique, concordance with histopathology was achieved in all cases. Regarding the cell‐block technique, only benign lesions (n = 40) were evaluated in the included studies, in which the concordance rate with histopathology was of 77.5%. The overall RoB judgment was “at risk of bias” for seven included studies mostly due to lack of information or reportedly absence of blinding of evaluator. Conclusions Within the limitations of this SR, FNAC presented modest to satisfactory diagnostic capability for the assessment of intraosseous jaw lesions, showing concordance rates higher than 64% for the smear and 77% for the cell‐block technique. Also, considerably high concordance rates were found for the assessment of malignant lesions regarding FNAC smear technique.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Diagnostic capability of fine‐needle aspiration cytology in assessment of intraosseous lesions of the jaws: A systematic review

Pilati

Santos

Melo

et al. 2020

Diagnostic Cytopathology

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“…This analysis can be performed after smearing the aspirated fluid directly onto a glass slide or using the cell block technique (CBT). [ 2 ] Fine-needle aspiration cytology (FNAC) of the superficial lesion or deep anatomical site is an increasingly common procedure in the diagnosis of neoplastic and cystic lesions. Sometimes, FNAC does not yield sufficient information for precise diagnosis, and the risk of false negative or intermediate diagnoses always exists.…”

Section: Introductionmentioning

confidence: 99%

“…An additional advantage of this technique is the possibility of executing immunohistochemistry (IHC) to identify the structures. [ 2 ] CBT are used as a complementary method in the diagnosis of odontogenic cystic lesions of the jaw. The aspiration of a cystic lesion can provide additional information about its content (if liquid or serous, or if absent) and aid in the presumptive clinical diagnosis at the moment of its application.…”

Section: Introductionmentioning

confidence: 99%

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Role of cell block technique as a novel diagnostic approach in odontogenic cysts and ameloblastomas of the jaw region

Sabarad

Abdulla

Chethana

et al. 2019

J Oral Maxillofac Pathol

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Cell‐block as ancillary tool for the diagnosis of cystic and cyst‐like jaw lesions

Melo

Mello

Mesquita

et al. 2020

Diagnostic Cytopathology

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BackgroundThe cell‐block technique is a cytological diagnostic method that decreases cellular dispersion; however, its usefulness regarding intraosseous lesions is underexplored. Therefore, this study aimed to assess the diagnostic usefulness of cell‐block for cystic and cyst‐like jaw lesions.MethodsIndividuals with cystic or cyst‐like jaw lesions who underwent aspiration biopsy were considered eligible. Aspiration of luminal content was prepared and processed by the cell‐block technique. Cytological evaluations were blindly performed by two trained PhD students (in a single evaluation) and one oral and maxillofacial pathologist (OMP). Cohen's kappa statistic was used to measure inter‐rater agreement.ResultsThe sample was composed of 52 lesions, represented by 25 radicular cysts (RC), 17 odontogenic keratocysts (OKC), 5 idiopathic bone cavities (IBC), and 5 unicystic ameloblastomas (UA). The kappa coefficient of cell‐block compared to histopathological diagnosis was 0.390 (95% confidence interval [95%CI], 0.195‐0.585) for PhD students and 0.612 (95%CI, 0.433‐0.791) for the OMP. The highest concordance rates between cell‐block and histopathological diagnosis were observed for RC (PhD = 76.0%; OMP = 80.0%) and OKC (PhD = 58.8%; OMP = 76.5%). Conflicting results were found regarding IBC (PhD = 40.0%; OMP = 80.0%) and UA, that presented the overall lowest concordance rates (PhD = 20.0%; OMP = 40.0%).ConclusionThe cell‐block technique presented a high diagnostic usefulness for detecting RC and OKC and, if associated with clinical and radiographic characteristics, might be sufficient for final diagnosis of these diseases. Regarding IBC and UA, an analysis with a higher number of cases is recommended to determine the true usefulness of the cell‐block as ancillary tool for the diagnosis of these lesions.

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Cell block technique as an additional tool in the diagnosis of ameloblastoma

Cited by 7 publications

References 14 publications

Diagnostic capability of fine‐needle aspiration cytology in assessment of intraosseous lesions of the jaws: A systematic review

Diagnostic capability of fine‐needle aspiration cytology in assessment of intraosseous lesions of the jaws: A systematic review

Role of cell block technique as a novel diagnostic approach in odontogenic cysts and ameloblastomas of the jaw region

Cell‐block as ancillary tool for the diagnosis of cystic and cyst‐like jaw lesions

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