Cell–Cell interactions of human neural progenitor-derived astrocytes within a microstructured 3D-scaffold

Führmann, Tobias; Hillen, Lisa M.; Montzka, Katrin; Wöltje, Michael; Brook, Gary

doi:10.1016/j.biomaterials.2010.06.060

Cited by 49 publications

(31 citation statements)

References 38 publications

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“…[ 15 , 16 ] In particular, polypyrrole (PPy) has been utilized in a number of in vitro studies investigating nerve, [ 17 ] muscle, [ 18 ] bone, [ 19 ] and endothelial cell growth. [ 20 ] Collectively, these studies reveal that the level of cytocompatibility and cell-material interaction may be modulated by the following external infl uences: (1) physical stimulation via controlled surface roughness, channels, or grooves; (2) biochemical stimulation via the release of biological growth factors; (3) mechanical stimulation and (4) electrical stimulation. Likewise, CNTs have been considered ideal candidate materials for bionics applications because of their inherent electrical conductivity and mechanical properties.…”

Section: Doi: 101002/adhm201200102mentioning

confidence: 99%

Electrical Stimulation of Myoblast Proliferation and Differentiation on Aligned Nanostructured Conductive Polymer Platforms

Quigley

Razal

Kita

et al. 2012

Adv Healthcare Materials

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Section: Doi: 101002/adhm201200102mentioning

confidence: 99%

Electrical Stimulation of Myoblast Proliferation and Differentiation on Aligned Nanostructured Conductive Polymer Platforms

Quigley

Razal

Kita

et al. 2012

Adv Healthcare Materials

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“…Cell-cell contact generally arrests cell growth through contact inhibition [14–17]. For example, relatively large islands of 2D substrates coated with the extracellular matrix (ECM) protein laminin promoted proliferation, while relatively small islands induced apoptosis [15, 17].…”

Section: Introductionmentioning

confidence: 99%

“…For example, relatively large islands of 2D substrates coated with the extracellular matrix (ECM) protein laminin promoted proliferation, while relatively small islands induced apoptosis [15, 17]. Similarly, cell-substrate and cell-cell interactions within 3D gel networks have been suggested to play a critical role in regulating cell proliferation, differentiation, and organ size [18–20].…”

Section: Introductionmentioning

confidence: 99%

Gel microstructure regulates proliferation and differentiation of MC3T3-E1 cells encapsulated in alginate beads

Lee

Guelcher

2012

Acta Biomaterialia

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For cell transplantation into damaged tissues, viable cells must be delivered to the defect site in a suitable carrier. However, the hypoxic and nutrient-limited environment in the carrier can induce massive cell death. The aims of this study were to increase the viability and regulate the behavior of osteoprogenitor cells encapsulated in alginate hydrogels through control of the gel microstructure. Cell survivability in alginate beads was improved through the use of α-MEM as the solvent for alginic acid sodium salt and CaCl2 solutions, which supplied additional nutrients for the cells compared to water or buffer. The mesh size and shear modulus of the hydrogel were hypothesized to regulate proliferation and differentiation of osteoprogenitor cells. MC3T3-E1 cells demonstrated enhanced osteoblast differentiation when encapsulated in high-density alginate with smaller mesh size and more rigid mechanical properties, as confirmed by increased alkaline phosphatase activity and osteocalcin secretion. However, MC3T3-E1 cells encapsulated in low-density alginate beads with a larger mesh size and more compliant mechanical properties exhibited increased proliferation. These results demonstrate that the microstructure of alginate hydrogels can regulate the behavior of osteoprogenitor cells, thus suggesting that the tuning the properties of the gel may be a useful approach for enhancing new bone formation.

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“…First, OEC cell viability after implantation of the collagen scaffold into the acute PNS lesion site was verified. In previous studies, the PM nerve guide was already tested for its biocompatibility with a variety of supportive cells, including rat and human SC, MSC, and astrocytes (Boecker et al, ; Bozkurt et al, ; Führmann, Hillen, Montzka, Wöltje, & Brook, ). By seeding GFP/OEC‐labelled cells on the PM nerve guide of GFP‐negative animals, the cell viability of OEC after 24 hr and 7 days could be clarified without being corrupted by any further freezing or staining procedure as well as a mechanical manipulation after explantation.…”

Section: Discussionmentioning

confidence: 99%

Cell-enrichment with olfactory ensheathing cells has limited local extra beneficial effects on nerve regeneration supported by the nerve guide Perimaix

Bozkurt

Kim

Altinova

et al. 2018

J Tissue Eng Regen Med

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The reconstruction of peripheral nerve injuries is clinically challenging, and today, the autologous nerve transplantation is still considered as the only gold standard remedy for nerve lesions where a direct nerve coaptation is not possible. Nevertheless, the functional merits of many biomaterials have been tested as potential substitutes for the autologous nerve transplant. One of the strategies that have been pursued is the combination of bioengineered nerve guides with cellular enrichment. In this present study, we combined the previously evaluated collagen-based and microstructured nerve guide Perimaix with olfactory ensheathing cell enrichment. Rat sciatic nerve defects of 20 mm were either bridged by a cell-seeded or nonseeded nerve guide or an autologous nerve transplant. Animals were monitored for 12 weeks for structural and functional parameters. Seeded cells survived on Perimaix, and following implantation aligned along the microstructured Perimaix framework. Axonal densities within the cell-seeded nerve guides were higher than in the nonseeded nerve guides and were comparable to the autograft. Additionally, cell-seeding had local beneficial effects on myelination within the nerve guide, as myelin sheath thickness was enhanced when compared with the empty scaffold. Nevertheless, for bridging the nerve gap of 20 mm, both the cell-seeded as well as nonseeded scaffolds were equally efficient regarding the functional outcome, which did not differ between the autograft, seeded or nonseeded groups. Our data demonstrate that olfactory ensheathing cell enrichment has local effects on nerve regeneration in combination with the Perimaix nerve guide. Surprisingly, for traversing the lesion gap, additional cell-seeding is not crucial.

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Cell–Cell interactions of human neural progenitor-derived astrocytes within a microstructured 3D-scaffold

Cited by 49 publications

References 38 publications

Electrical Stimulation of Myoblast Proliferation and Differentiation on Aligned Nanostructured Conductive Polymer Platforms

Electrical Stimulation of Myoblast Proliferation and Differentiation on Aligned Nanostructured Conductive Polymer Platforms

Gel microstructure regulates proliferation and differentiation of MC3T3-E1 cells encapsulated in alginate beads

Cell-enrichment with olfactory ensheathing cells has limited local extra beneficial effects on nerve regeneration supported by the nerve guide Perimaix

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