Cell communication: A cyclic-AMP mediated phenomenon

Hax, W.M.A.; Venrooij, Ger E.P.M. van; Vossenberg, J.B.J.

doi:10.1007/bf01869981

Cited by 82 publications

(22 citation statements)

References 45 publications

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“…18 and 33-37), but in only a few cases has direct phosphorylation of the channel molecule been demonstrated (33,34). Effects ofcAMP on electrical coupling between cells of a variety of tissues have been reported (1)(2)(3)(4)(5)(6)(7)(8)(9); in most cases, the onset ofthe effect is hours or many minutes after application, and in one case hours were required for an effect of cAMP-dependent protein kinase (38). An exception is the dopaminergic uncoupling in retinal horizontal cells of fish and turtle, which appears to be mediated by increase in cAMP.…”

Section: Discussionmentioning

confidence: 99%

“…Preliminary reports of these findings have appeared (15,16 4 min in a Microfuge, the supernatants were aspirated, and the pellets, containing the antibody-antigen complex, were washed in buffer A and centrifuged again. The resulting pellets were resuspended in NaDodSO4-containing solution (22) and aliquots were subjected to 12.5% NaDod-S04/PAGE.…”

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confidence: 97%

“…These slow effects are generally dependent on protein synthesis (2, 3, 6, 7), suggesting that the control is on formation of gap junctions. In a few cases, the effects of cAMP are more rapid, occurring within minutes to tens of minutes; examples include increases in coupling in insect salivary gland (4) and vertebrate heart muscle (5). Coupling between fish retinal horizontal cells is reversibly decreased by increases in cAMP with a time course fast enough to suggest a direct gating process (8,9).…”

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confidence: 99%

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cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide.

Sáez

Spray²,

Nairn

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

341

145

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Membrane-permeant cAMP derivatives (dibutyryl-and 8-bromo-cAMP) increase gap-junctional conductance within minutes when applied to voltage-clamped pairs of rat hepatocytes. Glucagon also increases junctional conductances, but the response has a more rapid onset and is more rapidly reversible. The glucagon effect can be prevented by intracellular injection of the protein inhibitor of the cAMPdependent protein kinase (Walsh inhibitor), indicating that the catalytic subunit of cAMP-dependent protein kinase is directly involved. The 27-kDa miijor gap junction polypeptide is phosphorylated when liver cells dissociated into small groups are incubated with 32p. Addition of 8-bromo-cAMP to cells increases the incorporation of 32P into the 27-kDa junctional protein. Serine is the amino acid residue that is phosphorylated. When isolated liver gap junctions are incubated in the presence of catalytic subunit of the cAMP-dependent protein kinase, the 27-kDa gap junction polypeptide is phosphorylated with low stoichiometry on serine. The rapid increases in gap junctional conductance caused by agents that elevate cAMP and phosphorylation of the gap junction protein by cAMPdependent protein kinase suggest that cAMP-dependent phosphorylation of the gap junction channel modulates the conductance of liver gap junctions.

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Section: Discussionmentioning

confidence: 99%

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confidence: 97%

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confidence: 99%

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cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide.

Sáez

Spray²,

Nairn

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

341

145

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“…An increase in intracellular cyclic AMP has been shown to speed transfer through gap junctions in a number of systems (Hax et al, 1974;Flagg-Newton et al, 1981;Saez et al, 1986; for review, see Spray and Bennett, 1985). The membrane permeant dibutyryl cyclic-AMP and the adenyl cyclase activator forskolin, both of which should increase intracellular cyclic AMP, were tested for a n effect on dye transfer through gap junctions in the early mouse embryo.…”

Section: Increasing Intracellular Cyclic Amp Speeds Gap Junctional Comentioning

confidence: 99%

Low intracellular pH is involved in the early embryonic death of DDK mouse eggs fertilized by alien sperm

Leclerc

Becker

Buehr

et al. 1994

Developmental Dynamics

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Intracellular pH was measured in normal 8-cell stage mouse embryos and in embryos from a cross between DDK females and C3H males. DDWC3H embryos display the DDK syndrome and spontaneously begin to decompact toward the late 16-cell stage. Ultimately, 90% fail to form blastocysts. Normal embryos have a resting intracellular pH close to neutrality. In DDKIC3H embryos a substantial proportion (46%) has an intracellular pH below 6.7. An equivalent proportion of DDK/C3H embryos was found previously to show slow communication through gap junctions at the 8-cell stage. This is probably a consequence of low intracellular pH. In normal embryos the weak acid, butyric acid, decreased intracellular pH and slowed the transfer of Lucifer Yellow through gap junctions. Normal embryos treated with butyrate for between 1 and 6 hr beginning at the 8-cell stage and cultured for 24 hr, reproduced the DDWC3H phenotype. After 48 hr some butyrate treated embryos recovered, while others remained as decompacted morulae. Treatment of control and DDWC3H 8-cell stage embryos with dibutyryl cyclic AMP or forskolin, which will increase intracellular cyclic AMP, speeded gap junctional communication. Forskolin treatment prevented expression of the DDK syndrome in DDWC3H embryos, although the rescue was transient and the syndrome returned when forskolin was removed. The finding that the DDK syndrome is manifested as low intracellular pH may provide clues to the molecular basis of the defect. o 1994 Wiley-Liss, Inc.

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“…Intracellular ATP is also believed to be associate with cell communication (26). Recently Hax et al (9) have postulated that the intracellular calcium and cyclic AMP produced from ATP by activation of adenyl cyclase are involved in regulation of cell communication. The role of Ca2+ and ATP in maintaining the structural rigidity of the cell membrane is also well known (1,4,5,22).…”

Section: Discussionmentioning

confidence: 99%

On Correlation between Gland Stiffness and Cell Coupling in Salivary Gland of <I>Chironomus plumosus</I> Larva

Suzuki

Sangworasil²,

Higashino

1978

Cell Struct. Funct.

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ABSTRACT. A correlation between gland stiffness and electrical cell coupling in the salivary gland of Chironomus plumosus was studied by electrophysiological and mechanical measurements. Local anaesthetics, such as alcohols, procaine and chlorpromazine increased gland stiffness. On the other hand, agents influencing cell membrane components, such as glycoletherdiaminetetraacetic acid (EGTA), lipase , trypsin, butylic acid and tetraethylammonium (TEA) decreased gland stiffness. These agents all produced intercellular uncoupling at high concentrations. Alkaline and low medium temperature led to increased gland stiffness and junctional uncoupling. Acid and high medium temperature induced decreased gland stiffness and junctional uncoupling. It was found that gland stiffness had an optimum range (11-27 dyne/cm) for maintenance of cell coupling.Cell communication plays an important role in the regulation of growth and division (7, 18). Loewenstein has postulated (16, 17) that Ca2+ takes part in this cell communication process. However, the role of Ca2+ and ATP in maintaining the structural rigidity of the cell membrane is also well known (1, 4, 5, 22). Intracellular ATP affects cell communication (26). We observed that impalement of gland cells on microelectrodes was either easier or harder depending on the kinds of uncoupling agents applied to the cells. Thus, we suspected that the mechanical properties of the surface of the gland may have been altered after treatment with these agents.We here present a correlation between the mechanical properties of the gland and electrical cell coupling. This was studied by mechanical and electrophysiological methods using the following drugs : local anaesthetics ; TEA and fatty acid as agents influencing the membrane permeability and/or the active transport of Na+, K+ and Ca2+; EGTA, lipase and trypsin as agents influencing the structural components of the cell membrane. The effects of pH and temperature were also examined.A preliminary report has appeared elsewhere (29). METHODSPreparation. Salivary gland cells of the third and fourth instar larvae of Chironomus plumo-161

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Cell communication: A cyclic-AMP mediated phenomenon

Cited by 82 publications

References 45 publications

cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide.

cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide.

Low intracellular pH is involved in the early embryonic death of DDK mouse eggs fertilized by alien sperm

On Correlation between Gland Stiffness and Cell Coupling in Salivary Gland of <I>Chironomus plumosus</I> Larva

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