Cell culture methods for testing Biocompatibility

Pizzoferrato, Alberto; Ciapetti, G.; Stea, Susanna; Cenni, E.; Arciola, Carla Renata; Granchi, Donatella; Lucia,

doi:10.1016/0267-6605(94)90081-7

Cited by 173 publications

(106 citation statements)

References 37 publications

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“…Bone cell culture systems have been largely used in studies with the goal of understanding biological events occurring at the bone tissue and biomaterial interface (2)(3)(4). Biocompatibility evaluations through cell cultures would be made using primary cell cultures because the biomaterials will interact with these cells after in vivo implantation (5).…”

Section: Introductionmentioning

confidence: 99%

Development of the osteoblast phenotype of serial cell subcultures from human bone marrow

Beloti

2005

Braz. Dent. J.

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Bone marrow cells have been used for testing biocompatibility of bone substitute materials that would be applied in maxillofacial and orthopedic surgeries. However, it remains unclear whether cells in serial subcultures retain the ability to differentiate into osteoblasts. The purpose of this study was to compare the development of osteoblast phenotype of serially passaged cells from human bone marrow. Cells from first to third passage were cultured (2x10 4 cells/well) in supplemented culture medium. Cells were incubated at 37ºC in a humidified atmosphere of 5% CO 2 and 95% air. Cell attachment was assessed at 4 and 24 h. At 7, 14 and 21 days, cell proliferation, cell viability, total protein content and alkaline phosphatase (ALP) activity were evaluated. Bone-like formation was evaluated at 14 and 21 days. Data were compared by two-way ANOVA and Duncan's multiple range test. Cell attachment, cell viability and total protein content were not affected by serial subcultures. However, serial subcultures did interfered negatively with osteoblast differentiation as shown by osteoblast parameters observed in second and third subcultures, such as continuous cell proliferation, lower ALP activity and bone-like formation in comparison to first subculture. Therefore, it is important to evaluate cell ability to growth and differentiate before selecting the cell population for studies that investigate the biocompatibility of materials to replace bone tissue.

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Section: Introductionmentioning

confidence: 99%

Development of the osteoblast phenotype of serial cell subcultures from human bone marrow

Beloti

2005

Braz. Dent. J.

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“…The biocompatibility was assessed by an in vitro model [20][21][22]; quantifying the adhesion, proliferation and viability of human osteoblasts cells in contact with the film surface in comparison with samples of medical grade stainless steel and tissue culture plastics, which represents the experimental optimum standard for investigations of osteoblast biology in vitro. Most probably, Nb thin films will not be the best material for real applications since Nb is considered a soft metal, but thin films based on Nb, such as Nb oxides or nitrides have a high potentiality, since they are hard, and wear and corrosion resistant [23,24].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of Niobium Coatings

Olaya²,

et al. 2011

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Niobium coatings deposited by magnetron sputtering were evaluated as a possible surface modification for stainless steel (SS) substrates in biomedical implants. The Nb coatings were deposited on 15 mm diameter stainless steel substrates having an average surface roughness of 2 µm. To evaluate the biocompatibility of the coatings three different in vitro tests, using human alveolar bone derived cells, were performed: cellular adhesion, proliferation and viability. Stainless steel substrates and tissue culture plastic were also studied, in order to give comparative information. No toxic response was observed for any of the surfaces, indicating that the Nb coatings act as a biocompatible, bioinert material. Cell morphology was also studied by immune-fluorescence and the results confirmed the healthy state of the cells on the Nb surface. X-ray diffraction analysis of the coating shows that the film is polycrystalline with a body centered cubic structure. The surface composition and corrosion resistance of both the substrate and the Nb coating were also studied by X-ray photoelectron spectroscopy and potentiodynamic tests. Water contact angle measurements showed that the Nb surface is more hydrophobic than the SS substrate. OPEN ACCESSCoatings 2011, 1 73

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“…Цитотоксичность материала и жизнеспособ-ность клеток Еще в конце двадцатого века были описаны основные методики оценки цитотоксичности [15][16][17], а также опубликованы стандарты по их ис-пользованию, такие как, например, Американско-го общества по испытанию материалов (American Society for Testing and Materials -ASTM) [18] и Международной организации по стандартизации (International Standards Organization -ISO) [19]. Принято считать, что цитотоксичность, опреде-ляемая как свойство химических веществ оказы-вать токсическое действие на клетки, затрагивает различные аспекты клеточной функции, такие как жизнеспособность, пролиферация, адгезия, био-синтетическая активность и морфология клетки.…”

Section: параметры биосовместимости оцениваемые в тестах In Vitrounclassified

“…Целью такого анализа является определение физиологической или значимой патологической продукции белков [42]. Изучение активации онкогенеза и утраты про-дуктов генов-онкосупрессоров может быть крайне полезным для in vitro тестирования канцерогенно-го потенциала биоматериалов [15].…”

Section: генотоксичностьunclassified