Cell Culture of <i>Bursera Linanoe</i> in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

Pavón-Reyes, Leticia; Evangelista‐Lozano, Silvia; Sepúlveda-Jiménez, Gabriela; Avila, Víctor Chávez; Rodrı́guez-Monroy, Mario

doi:10.1177/1934578x1701200301

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…Maximal biomass of cell suspension in culture from S. angustifolia obtained in the bioreactor was 1.42-fold superior to that generated in the cultures developed in Erlenmeyer flasks [11]; nevertheless, the max was lowest. This result is consistent with that described for other species, such as B. vulgaris [13], A. indica [15], and Burcera linanoe [22]; maximal biomasses were obtained in the stirred tank-type bioreactor rather than in Erlenmeyer flasks. The stirred bioreactor tank is considered a good option for plant cell culture.…”

Section: Resultssupporting

confidence: 91%

Production of anti‐inflammatory compounds in Sphaeralcea angustifolia cell suspension cultivated in stirred tank bioreactor

Pérez-Hernández

Nicasio-Torres

Sarmiento-López

et al. 2019

Engineering in Life Sciences

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Sphaeralcea angustifolia is a plant used for the treatment of inflammatory processes. Scopoletin, tomentin, and sphaeralcic acid were identified as the compounds with anti‐inflammatory and immunomodulatory effects. Successful establishment of the cell culture in Erlenmeyer flasks has been reported previously. The aim of this study was to evaluate the ability of cells in suspension from S. angustifolia grown in a stirred tank bioreactor and demonstrate their capacity to produce bioactive compounds. Cells in suspension grown at 200 rpm reached a maximal cell biomass in dry weight at 19.11 g/L and produced 3.47 mg/g of sphaeralcic acid. The mixture of scopoletin and tomentin was only detected at the beginning of the culture (12.13 μg/g). Considering that the profile of dissolved oxygen during the cultures was lesser than 15%, it is possible that the low growth at 100 rpm could be due to oxygen limitations or to cell sedimentation. At 400 rpm, a negative effect on cell viability could be caused by the increase in the hydrodynamic stress, including the impeller tip, average shear rate, and Reynolds number. The sphaeralcic acid content in the cell suspension of S. angustifolia obtained in the bioreactor was two orders of magnitude greater than that reported for the culture grown in Erlenmeyer flasks.

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Section: Resultssupporting

confidence: 91%

Production of anti‐inflammatory compounds in Sphaeralcea angustifolia cell suspension cultivated in stirred tank bioreactor

Pérez-Hernández

Nicasio-Torres

Sarmiento-López

et al. 2019

Engineering in Life Sciences

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“…It has been reported that the use of some auxins to obtain callus in the genus Bursera, such as indole butyric acid (IBA) and naphthalene acetic acid (NAA), allowed the formation of 25-87% of callus [27,31,32].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Profiles and Cytotoxic Activity of Bursera fagaroides Leaves and Its Callus Culture

Pérez-Mejía,

Villarreal,

Sánchez-Carranza

et al. 2024

Preprint

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Bursera fagaroides popularly used in México possess bioactive lignans. These compounds are low in the bark, and its extraction endangers the life of the trees. The aim of the present investigation was to search for alternative sources of cytotoxic compounds from B. fagaroides as leaves and in vitro callus cultures. The friable callus of B. fagaroides were established using a combination of phytoregulators: 4 mgL-1 of 2,4-dichlorophenoxyacetic acid (2,4-D), 1 mgL-1 Naphthaleneacetic Acid (NAA) and 1 mgL-1 Zeatin. The maximum cell growth was at day 28 with a specific growth rate μ= 0.059 days-1 and duplication time td=11.8 days. Scopoletin with a concentration of 10.7 µg/g dry weight was the main compound inducible as a phytoalexin by the addition of high concentrations of 2,4-D, as well as by the absence of nutrients in the culture medium. The com-pounds γ-sitosterol, and stigmasterol were also present. Open column chromatography was used to separate and identify: yatein, acetyl podophyllotoxin and 7'8'-dehydropodophyllotoxin in the leaves of the wild plant. Cytotoxic activity on four cancer cell lines was tested, being PC-3 prostate carcinoma (IC50 of 12.6±4.6) the most sensitive to the wild-type plant extract and HeLa cervical carcinoma (IC50 of 72±5) to the callus culture extract.

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“…On the other hand, the application of 1.5 mgL −1 of indol butyric acid (IBA) over stem explants of B. laxiflora allowed the induction of 80% of callus after 30 days [ 35 ]. In another work, using the combination of naphthalene acetic acid (NAA, 3 mgL −1 ) and 6-benzylaminopurine (BAP, 0.5 mgL −1 ), 95% of callus induction was obtained from B. linaloe at 40 days of treatment [ 36 ]. Among the different types of auxins is 2,4-D, a synthetic auxin that causes rapid cell proliferation, formation and optimal callus growth.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Profiles and Cytotoxic Activity of Bursera fagaroides (Kunth) Engl. Leaves and Its Callus Culture

Pérez-Mejía,

Villarreal,

Sánchez-Carranza

et al. 2024

Plants

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Bursera fagaroides, popularly used in México, possesses bioactive lignans. These compounds are low in the bark, and its extraction endangers the life of the trees. The aim of the present investigation was to search for alternative sources of cytotoxic compounds in B. fagaroides prepared as leaves and in vitro callus cultures. The friable callus of B. fagaroides was established using a combination of plant growth regulators: 4 mgL−1 of 2,4-dichlorophenoxyacetic acid (2,4-D), 1 mgL−1 Naphthaleneacetic Acid (NAA) and 1 mgL−1 Zeatin. The maximum cell growth was at day 28 with a specific growth rate of μ = 0.059 days−1 and duplication time td = 11.8 days. HPLC quantification of the dichloromethane callus biomass extract showed that Scopoletin, with a concentration of 10.7 µg g−1 dry weight, was the main compound inducible as a phytoalexin by the addition of high concentrations of 2,4-D, as well as by the absence of nutrients in the culture medium. In this same extract, the compounds γ-sitosterol and stigmasterol were also identified by GC-MS analysis. Open column chromatography was used to separate and identify yatein, acetyl podophyllotoxin and 7′,8′-dehydropodophyllotoxin in the leaves of the wild plant. Cytotoxic activity on four cancer cell lines was tested, with PC-3 prostate carcinoma (IC50 of 12.6 ± 4.6 µgmL−1) being the most sensitive to the wild-type plant extract and HeLa cervical carcinoma (IC50 of 72 ± 5 µgmL−1) being the most sensitive to the callus culture extract.

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Cell Culture of Bursera Linanoe in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

Cited by 4 publications

References 18 publications

Production of anti‐inflammatory compounds in Sphaeralcea angustifolia cell suspension cultivated in stirred tank bioreactor

Production of anti‐inflammatory compounds in Sphaeralcea angustifolia cell suspension cultivated in stirred tank bioreactor

Phytochemical Profiles and Cytotoxic Activity of Bursera fagaroides Leaves and Its Callus Culture

Phytochemical Profiles and Cytotoxic Activity of Bursera fagaroides (Kunth) Engl. Leaves and Its Callus Culture

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