Cell Cycle Arrest Activity of Litsea Cubeba Lour: Heartwood and Fruit Extracts Against T47d Breast Cancer Cells

Abstract

Objective: This study was carried out to investigate cell cycle arrest activity toward T47D cells of Litsea cubeba heartwoods and fruits extract.Methods: Dry extracts were prepared from dry-grounded heartwoods and fruits by cold maceration using n-hexane, ethylacetate, and ethanol solvent. Cytotoxic activity were measured by [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] methods and interpreted with inhibitory concentration 50% (IC 50 ) value. Cell cycle arrest was investigated by flowcytometry method. Results:IC 50 values for each n-hexane, ethylacetate, and ethanol of L. cubeba heartwoods and fruits were 76.34±2.61; 67.52±2.45; 71.23±2.37; 75.59±3.24; 162.58±15.08; 63.70±2.67 µg/mL, respectively. Cell cycle arrest for ethylacetate extract of heartwoods and fruits were accumulated in G 0 /G 1 phase (56.70% and 65.56%). Conclusion:These results suggest that L. cubeba heartwoods and fruits has cell cycle arrest activity.

The composition, pharmacological and economic importance of essential oil of Litsea cubeba (Lour.) Pers

“…n-hexane, ethyl acetate, and ethanol extracts of heartwood and fruits of L. cubeba were tested for antiproliferative activity against breast cancer cells. The results showed that all tested extracts arrested the progress of the cancer cell cycle with different degrees of IC 50 values, ranging from 63-162 µg/mL (Dalimunthe et al, 2017). The results suggested that L. cubeba extracts inhibit the proliferation of cancer cells.…”

AKTIVITAS PENGHAMBATAN ENZIM PROTEASE 6LU7 VIRUS SARS-COV-2 OLEH SENYAWA ALKALOID DARI ATTARASA (Litsea cubeba (Lour.)Pers.) SECARA IN-SILICO

“…Tumbuhan ini memiliki diameter batang 6-20 cm, serta tinggi tumbuhan 5-10 m [10]. Buah Attarasa mengandung berbagai metabolit sekunder seperti alkaloid, flavonoid, saponin, dan tanin [11] senyawa alkaloid yang berkhasiat sebagai anti diare, anti diabetes, anti mikroba dan anti malaria, akan tetapi beberapa senyawa golongan alkaloid bersifat racun sehingga diperlukan adanya identifikasi senyawa golongan alkaloid yang dapat diketahui manfaatnya. Alkaloid adalah senyawa metabolit sekunder terbanyak yang memiliki atom nitrogen, yang ditemukan dalam jaringan tumbuhan dan hewan.…”

Cytotoxic Activity of BornUSU I towards T47D Breast Cancer Cells

“…After incubation, absorbance was measured using ELISA reader at λ 595 nm. The data which were absorbed from each well were converted to percentage of viable cells [5], [6], [7], [8], [9]. The equation to determine viability of cells:…”