Cell cycle arrest of S. cerevisiae in conjunction with labeling of the cell wall

Tartakoff, Alan M.

doi:10.1016/j.xpro.2021.100646

Cited by 1 publication

(1 citation statement)

References 16 publications

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“…In the current study, the antitumor mechanism of Morusin was investigated in Huh7 and Hep3B cells in association with glycolysis and G1 arrest. Usually MTT and CCK-8 assays have been used for short term cytotoxicity [ 19 , 20 ], while colony formation has been applied for long term cytotoxicity [ 21 , 22 ]. Here, Morusin significantly showed cytotoxicity by MTT and CCK-8 assays and abrogated the number of colonies in Huh7 and Hep3B cells, implying the antitumor potency of Morusin.…”

Section: Discussionmentioning

confidence: 99%

Antitumor Effect of Morusin via G1 Arrest and Antiglycolysis by AMPK Activation in Hepatocellular Cancer

Cho

Park

Lee

et al. 2021

IJMS

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Though Morusin isolated from the root of Morus alba was known to have antioxidant, anti-inflammatory, antiangiogenic, antimigratory, and apoptotic effects, the underlying antitumor effect of Morusin is not fully understood on the glycolysis of liver cancers. Hence, in the current study, the antitumor mechanism of Morusin was explored in Hep3B and Huh7 hepatocellular carcninomas (HCC) in association with glycolysis and G1 arrest. Herein, Morusin significantly reduced the viability and the number of colonies in Hep3B and Huh7 cells. Moreover, Morusin significantly increased G1 arrest, attenuated the expression of cyclin D1, cyclin D3, cyclin E, cyclin-dependent kinase 2 (CDK2), cyclin-dependent kinase 4 (CDK4), and cyclin-dependent kinase 6 (CDK6) and upregulated p21 and p27 in Hep3B and Huh7 cells. Interestingly, Morusin significantly activated phosphorylation of the adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) but attenuated the expression of the p-mammalian target of protein kinase B (AKT), rapamycin (mTOR), c-Myc, hexokinase 2(HK2), pyruvate kinases type M2 (PKM2), and lactate dehydrogenase (LDH) in Hep3B and Huh7 cells. Consistently, Morusin suppressed lactate, glucose, and adenosine triphosphate (ATP) in Hep3B and Huh7 cells. Conversely, the AMPK inhibitor compound C reduced the ability of Morusin to activate AMPK and attenuate the expression of p-mTOR, HK2, PKM2, and LDH-A and suppressed G1 arrest induced by Morusin in Hep3B cells. Overall, these findings suggest that Morusin exerts an antitumor effect in HCCs via AMPK mediated G1 arrest and antiglycolysis as a potent dietary anticancer candidate.

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Section: Discussionmentioning

confidence: 99%