Cell-Degeneration Pattern in Bone Marrow and Blood of Guinea Pigs Following Administration of Cyclophosphamide

Kaul, M.K.; Hudson, G

doi:10.1159/000208760

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…Howev er, the apparent depletion of the eosinophil population at 24 h would be consistent with a similar depletion of blood eosinophils noted at this and subsequent intervals in the guinea-pig [6,7].…”

Section: Discussionsupporting

confidence: 78%

“…Corresponding test and control animals were studied concurrently. The general effect on the test animals was similar to that described earlier [6,7] in that about 10% of the body weight was lost over the first 3 days when the animals were a little off-colour, but thereafter no ill effects were apparent. 10 test and 10 control ani mals were studied at each of the following intervals after injection: 6, 12 and 24 h, 3, 6, 9, 12, 18, 24 and 30 days.…”

supporting

confidence: 80%

“…The apparent 'sensitivity' of the lymphoid population to cyclophos phamide is parallel to that already observed in the Peyer's patch [1] and bone marrow [5][6][7]. Bearing in mind that amongst the complex actions of alkylating agents [13] effects on DNA synthesis appear to be very impor tant, one might expect particular effects on actively dividing cells and sec ondarily on cell populations derived from them.…”

Section: Discussionsupporting

confidence: 53%

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Quantitative Study of the Effects of Cyclophosphamide on Peritoneal Cell Populations

Chin¹,

Hudson²

1972

Acta Haematol

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A quantitative study of peritoneal cell populations has been carried out in mice at intervals of 6 h to 30 days after a single sublethal dose of cyclophosphamide. There was a severe depletion in the number of lymphoid cells, which was first detected at 12 h and reached a maximum at 6 days. Macrophages were evident in the removal and disposal of degenerating cells, and showed increased numbers at 3 days. Recovery was rather slow, significant differences in small lymphocyte counts being still present at 18 days, and in other lymphocytes and blast cells at even later intervals.

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Section: Discussionsupporting

confidence: 78%

supporting

confidence: 80%

Section: Discussionsupporting

confidence: 53%

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Quantitative Study of the Effects of Cyclophosphamide on Peritoneal Cell Populations

Chin¹,

Hudson²

1972

Acta Haematol

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“…To study the effect of neutrophils on agarose plug-induced goblet cell metaplasia, the rats were pretreated with cyclophosphamide (an inhibitor of bone marrow leukocytes but not of monocytes) (17). Cyclophosphamide (100 mg/kg ip) was given 5 days before instillation of the agarose plugs, and a second injection of cyclophosphamide (50 mg/kg ip) was given 1 day before instillation of the plugs.…”

Section: Animalsmentioning

confidence: 99%

Agarose plug instillation causes goblet cell metaplasia by activating EGF receptors in rat airways

Lee

Takamatsu

Dabbagh

et al. 2000

American Journal of Physiology-Lung Cellular and Molecular Phys

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We hypothesized that foreign bodies in airways cause inflammation leading to goblet cell metaplasia. Instilled agarose plugs lodged in the bronchi of pathogen-free rats caused a time-dependent increase in Alcian blue-periodic acid-Schiff staining that was detected within 24 h and markedly increased at 72 h. Control bronchi contained no pregoblet or goblet cells, but plugged bronchi contained many pregoblet and goblet cells and a decrease in nongranulated secretory cells. In situ hybridization showed no expression of MUC5AC in control airways, but plugged airways showed a marked expression. Control bronchi showed sparse staining for epidermal growth factor receptor (EGFR) protein, but plugged bronchi showed intense EGFR staining in the epithelium. Pretreatment with an EGFR tyrosine kinase inhibitor (BIBX1522) prevented Alcian blue-periodic acid-Schiff staining and MUC5AC gene expression in plugged bronchi. Pretreatment with tumor necrosis factor-alpha neutralizing antibody or pretreatment with cyclophosphamide abolished plug-induced EGFR protein expression and goblet cell metaplasia. Thus instillation of agarose plugs induces profound goblet cell metaplasia by causing EGFR expression and activation.

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Morphological changes induced by cyclophosphamide in crypt epithelium of the small intestine in mice: Light and electron microscopic studies

1977

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When a single sublethal dose (300 mglkg body weight) of cyclophosphamide was injected intraperitoneally into experimental mice, several morphological alterations were detected in the crypt epithelium within a few hours. These were (a) mitotic activity of epithelial cells decreased, reaching the lowest level a t 16 hours; (b) an abnormally high number of lymphocytes and cells with eosinophilic granules appeared in the epithelium; later these cells degenerated and the maximum number of dead cells was observed a t six hours after the drug injection. At four days post-injection the morphology of crypt epithelium had returned to normal.Electron microscopic examination revealed that although the mitotic activity of crypt epithelial cells was depressed, there was little change in their morphology following the drug administration. However, many intermediate stages of degeneration of lymphocytes and eosinophilic cells were observed in the intercellular spaces as well as apparently inside the cytoplasm of crypt epithelial cells. The eosinophilic cells are characterized by the presence of large crystal-containing granules in the cytoplasm, and are believed to be equivalent to "globular leucocytes" described by many investigators. Evidence from this study suggests that lymphocytes and globular leucocytes are more sensitive to cyclophosphamide than crypt epithelial cells, and that they degenerate in crypt epithelium.

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Cell-Degeneration Pattern in Bone Marrow and Blood of Guinea Pigs Following Administration of Cyclophosphamide

Cited by 5 publications

References 7 publications

Quantitative Study of the Effects of Cyclophosphamide on Peritoneal Cell Populations

Quantitative Study of the Effects of Cyclophosphamide on Peritoneal Cell Populations

Agarose plug instillation causes goblet cell metaplasia by activating EGF receptors in rat airways

Morphological changes induced by cyclophosphamide in crypt epithelium of the small intestine in mice: Light and electron microscopic studies

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