Cell Division: Key to Cellular Morphogenesis in the Fission Yeast, Schizosaccharomyces

Johnson, Byron F.; Calleja, G. B.; Yoo, Bong Y.; Zuker, Michael; McDonald, I.

doi:10.1016/s0074-7696(08)61004-9

Cited by 38 publications

(27 citation statements)

References 50 publications

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“…In fission yeast, the septum is a three-layered structure that is synthesized in two steps (Johnson et al, 1982). The first part to be laid down in a centripetal manner is the primary septum, which is rich in linear ␤-1,3-glucan (Horisberger and Rouver-Vauthey, 1985;Humbel et al, 2001), and its deposition is dependent on constriction of the actomyosin ring (Liu et al, 1999(Liu et al, , 2000.…”

Section: Discussionmentioning

confidence: 99%

“…For transmission electron microscopy (TEM), the cells were stained with potassium permanganate according to the protocol described by Johnson et al (1982). Electron photomicrographs were taken with a Jeol Jem-1010 electron microscope (Peabody, MA).…”

Section: Microscopy Techniquesmentioning

confidence: 99%

“…Genetic studies have indicated that activation of the SIN pathway might regulate Cps1p, a ␤-1,3-glucan synthase subunit essential for the assembly of the division septum (Le Goff et al, 1999;Liu et al, 2000). This septum has a three-layer structure (Johnson et al, 1982), with a central primary septum (mainly composed of linear ␤-1,3-glucan) surrounded on both sides by two secondary septa (composed of ␤-1,6-branched ␤-1,3-glucan, and ␤-1,6-glucan; Humbel et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Ace2p Controls the Expression of Genes Required for Cell Separation inSchizosaccharomyces pombe

Alonso-Nuñez

Martín‐Cuadrado

et al. 2005

MBoC

125

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Schizosaccharomyces pombe cells divide by medial fission through contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Here we describe the identification of seven genes (adg1؉ , and mid2 ؉ ) whose expression is induced by the transcription factor Ace2p. The expression of all of these genes varied during the cell cycle, maximum transcription being observed during septation. At least three of these proteins (Eng1p, Agn1p, and Cfh4p) localize to a ring-like structure that surrounds the septum region during cell separation. Deletion of the previously uncharacterized genes was not lethal to the cells, but produced defects or delays in cell separation to different extents. Electron microscopic observation of mutant cells indicated that the most severe defect is found in eng1⌬ agn1⌬ cells, lacking the Eng1p endo-␤-1,3-glucanase and the Agn1p endo-␣-glucanase. The phenotype of this mutant closely resembled that of ace2⌬ mutants, forming branched chains of cells. This suggests that these two proteins are the main activities required for cell separation to be completed.

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Section: Discussionmentioning

confidence: 99%

Section: Microscopy Techniquesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ace2p Controls the Expression of Genes Required for Cell Separation inSchizosaccharomyces pombe

Alonso-Nuñez

Martín‐Cuadrado

et al. 2005

MBoC

125

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“…However, the degree of morphological similarity between the S. cerevisiae and mammalian secretory organelles (especially the Golgi compartments) has been in doubt. To Golgi complexes observed in animal cells (Novikoff et al, 1971;Rambourg et al, 1981) or the multi-copy compartments observed in other yeasts, such as S. pombe (Johnson et al, 1982;Chappel and Warren, 1989), the S. cerevisiae compartments typically are not organized into parallel stacks of membranes. Clusters of three cistemae occur relatively infrequently (' 10% of the Golgi bodies consist of such stacks), and larger stacks were not observed.…”

Section: Discussionmentioning

confidence: 99%

“…Animal cells typically contain one Golgi complex (Novikoff et al, 1971;Rambourg et al, 1981) that consists of several parallel and functionally distinct membranous sacs in which the oligosaccharides that decorate secretory proteins are successively extended and modified (reviewed in Kornfeld and Kornfeld 1985). Although stacked cisternal compartments (reminiscent of the mammalian Golgi complex) can be readily observed in other yeast species, such as Schizosaccharomyces pombe (Johnson et al, 1982;Chappel and Warren, 1989), few of these distinctive structures have been described in S. cerevisiae.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy.

Preuss

Mulholland

Franzusoff

et al. 1992

MBoC

263

221

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The membrane compartments responsible for Golgi functions in wild-type Saccharomyces cerevisiae were identified and characterized by immunoelectron microscopy. Using improved fixation methods, Golgi compartments were identified by labeling with antibodies specific for alpha 1-6 mannose linkages, the Sec7 protein, or the Ypt1 protein. The compartments labeled by each of these antibodies appear as disk-like structures that are apparently surrounded by small vesicles. Yeast Golgi typically are seen as single, isolated cisternae, generally not arranged into parallel stacks. The location of the Golgi structures was monitored by immunoelectron microscopy through the yeast cell cycle. Several Golgi compartments, apparently randomly distributed, were always observed in mother cells. During the initiation of new daughter cells, additional Golgi structures cluster just below the site of bud emergence. These Golgi enter daughter cells at an early stage, raising the possibility that much of the bud's growth might be due to secretory vesicles formed as well as consumed entirely within the daughter. During cytokinesis, the Golgi compartments are concentrated near the site of cell wall synthesis. Clustering of Golgi both at the site of bud formation and at the cell septum suggests that these organelles might be directed toward sites of rapid cell surface growth.

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Eleven novelsep genes ofSchizosaccharomyces pombe required for efficient cell separation and sexual differentiation

Grallert

Grallert²,

Zilahi

et al. 1999

Yeast

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Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9‐307, sep14‐576 and sep15‐598 showed genetic interactions with sep1‐1, a mutation in a forkhead transcription factor homologue. Additional genetic interactions were detected between sep6‐194, sep15‐598 and cdc16‐116, a mutant allele of an anaphase modulator of p34cdc2. sep9‐307 and sep15‐598 caused dikaryosis in wee1− background. In mating and sporulation tests, sep6−, sep7−, sep9−, sep10−, sep11− and sep15− proved to be defective in conjugation only, whereas sep8−, sep13− and sep16− were also defective in meiosis‐sporulation. sep12− and sep14− were only partially sterile. All mutants could produce M‐factor but sep8−, sep11−, sep15− and sep16− were defective in P‐factor production. The mutations in sep8, sep11 and sep16 suppressed the pat1‐114‐driven meiosis. All mutants were sensitive to the presence of higher concentrations of chloride in the medium and to short heat shocks. The diversity of the mutant phenotypes and the pleiotropic effects of the mutations suggest that these sep genes might act in, or interact with, a multiple overlapping network of regulatory modules. Copyright © 1999 John Wiley & Sons, Ltd.

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Cell Division: Key to Cellular Morphogenesis in the Fission Yeast, Schizosaccharomyces

Cited by 38 publications

References 50 publications

Ace2p Controls the Expression of Genes Required for Cell Separation inSchizosaccharomyces pombe

Ace2p Controls the Expression of Genes Required for Cell Separation inSchizosaccharomyces pombe

Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy.

Eleven novelsep genes ofSchizosaccharomyces pombe required for efficient cell separation and sexual differentiation

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