Cell encapsulation: Overcoming barriers in cell transplantation in diabetes and beyond

Farina, Marco; Alexander, Jenolyn F.; Thekkedath, Usha; Ferrari, Mauro; Grattoni, Alessandro

doi:10.1016/j.addr.2018.04.018

Cited by 152 publications

(133 citation statements)

References 233 publications

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“…Mechanical instability of previous capsule systems was well‐established and presented a sizable hurdle for the field of encapsulated cell transplantation . Our study results substantiated those observed in recent gel reinforcement studies, particularly the noticeable change in mechanics following the incorporation of an interpenetrating polymer network .…”

Section: Discussionsupporting

confidence: 87%

Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation

Verheyen

Morales

Sussman

et al. 2019

Macro Materials & Eng

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Islet transplantation within mechanically stable microcapsules offers the promise of long‐term diabetes reversal without chronic immunosuppression. Reinforcing the ionically gelled network of alginate (ALG) hydrogels with covalently linked polyethylene glycol (PEG) may create hybrid structures with desirable mechanical properties. This report describes the fabrication of hybrid PEG‐ALG interpenetrating polymer networks and the investigation of microcapsule swelling, surface modulus, rheology, compression, and permeability. It is demonstrated that hybrid networks are more resistant to bulk swelling and compressive deformation and display improved shape recovery and long‐term resilience. Interestingly, it is shown that PEG‐ALG networks behave like ALG during microscale surface deformation and small amplitude shear while exhibiting similar permeability properties. The results from this report's in vitro characterization are interpreted according to viscoelastic polymer theory and provide new insight into hybrid hydrogel mechanical behavior. This new understanding of PEG‐ALG mechanical performance is then linked to previous work that demonstrated the success of hybrid polymer immunoisolation devices in vivo.

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Section: Discussionsupporting

confidence: 87%

Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation

Verheyen

Morales

Sussman

et al. 2019

Macro Materials & Eng

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“…Lipid encapsulation of small interfering RNAs are deliverable to macrophages to knockdown the inflammatory response of monocytes and dendritic cells in vivo [263], and this type of delivery system could be adapted to alter molecular mechanisms within immune cells that over respond to activation from stress. Additional studies are examining the promise of transplanting engineered cells, encapsulated in order to prevent rejection, to treat diabetes, blood disease, and cancer [264]. Engineering encapsulated pluripotent bone marrow stem cells could also be used to target the development of an entire immune system.…”

Section: Future Research Directionsmentioning

confidence: 99%

Inflaming sex differences in mood disorders

Rainville

Hodes

2018

Neuropsychopharmacol

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Men and women often experience different symptoms or rates of occurrence for a variety of mood disorders. Many of the symptoms of mood disorders overlap with autoimmune disorders, which also have a higher prevalence in women. There is a growing interest in exploring the immune system to provide biomarkers for diagnosis of mood disorders, along with new targets for developing treatments. This review examines known sex differences in the immune system and their relationship to mood disorders. We focus on immune alterations associated with unipolar depression, bipolar depression, and anxiety disorders. We describe work from both basic and clinical research examining potential immune mechanisms thought to contribute to stress susceptibility and associated mood disorders. We propose that sex and age are important, intertwined factors that need to be included in future experimental designs if we are going to harness the power of the immune system to develop a new wave of treatments for mood disorders.

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“…This technique was introduced by Lim and Sun in 1980 and has been continuously developed and adapted since then. Recent reviews summarized advances in the encapsulation methods . This strategy has the potential to overcome graft rejection without the need for long‐term immunosuppressive medication, thus avoiding related side effects .…”

Section: In Vivo Studies In Pigs—the Bioartificial Pancreas (Bap)mentioning

confidence: 99%

Integration of nano‐ and biotechnology for beta‐cell and islet transplantation in type‐1 diabetes treatment

et al. 2020

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Regenerative medicine using human or porcine β-cells or islets has an excellent potential to become a clinically relevant method for the treatment of type-1 diabetes.High-resolution imaging of the function and faith of transplanted porcine pancreatic islets and human stem cell-derived beta cells in large animals and patients for testing advanced therapy medicinal products (ATMPs) is a currently unmet need for pre-clinical/clinical trials. The iNanoBIT EU H2020 project is developing novel highly sensitive nanotechnology-based imaging approaches allowing for monitoring of survival, engraftment, proliferation, function and whole-body distribution of the cellular transplants in a porcine diabetes model with excellent translational potential to humans. We develop and validate the application of single-photon emission computed tomography (SPECT) and optoacoustic imaging technologies in a transgenic insulindeficient pig model to observe transplanted porcine xeno-islets and in vitro differentiated human beta cells. We are progressing in generating new transgenic reporter pigs and human-induced pluripotent cell (iPSC) lines for optoacoustic imaging and

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Cell encapsulation: Overcoming barriers in cell transplantation in diabetes and beyond

Cited by 152 publications

References 233 publications

Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation

Characterization of Polyethylene Glycol–Reinforced Alginate Microcapsules for Mechanically Stable Cell Immunoisolation

Inflaming sex differences in mood disorders

Integration of nano‐ and biotechnology for beta‐cell and islet transplantation in type‐1 diabetes treatment

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