Cell features in pancreas of prediabetic and diabetic rabbits after wirsung duct ligation. Histochemical and histoenzymatic studies

Abstract

Kinetic studies of the histochemical and histoenzymatic behavior of rabbit pancreatic parenchymas were performed 5, 30 and 90 days after Wirsung duct ligation. In control pancreas, some enzyme activities (EA) were more prominent in Langerhans islets [glucose-6-phosphatase, glucose-6-phosphate dehydrogenase (DH), isocitrate DH, glycerol-3-phosphate DH, NADPH DH], others were strongly marked in acini and ducts (alkaline phosphatase, beta-glucuronidase, acid esterase aryl-sulfatase). Histochemical and enzyme abnormalities observed in experimental rabbits reflect the post-ligation degenerative and reactive processes in both exocrine and endocrine pancreas: (1) the decrease in Krebs cycle and pentose pathway linked EA and the increased lysosomal and acid phosphatase EA reflect early (day 5) degeneration and necrosis of islets and acini (day 30); (2) proliferative processes in developed ductal epithelia are shown by an increase in both glycolytic and lysosomal EA (days 30 and 90); (3) connective tissue neogenesis and interstitial fibrosis occurred as shown by activated beta-glucuronidase, aryl-sulfatase, alkaline phosphatase and increased ribonucleoproteins and glycoaminoglycans contents (day 30); (4) on day 90, the neoformed cell clusters presenting glucose-6-phosphatase positivity (B-cell marker) are seen in the pancreas remnant. At the same time, blood insulin level increases correlated with a decrease of hyperglycemia.

Changes in the Mouse Exocrine Pancreas after Pancreatic Duct Ligation: A Qualitative and Quantitative Histological Study.

“…Many morphological studies have dealt with the pancreas of various animals after duct obstruction either by ligation or injection of foreign materials such as microspheres or high-viscosity solutions into the duct (EDLUND et al, 1962;SATAKE et al, 1983;EKBERG et al, 1988;KLEMPNAUER et al, 1990;CATALA et al, 1990). The findings in those studies, however, depended on limited fields of thin sections, lacking in extensive and three-dimensional information concerning the whole organ.…”

Insulin and Glucagon Impairments in Relation with Islet Cells Morphological Modifications Following Long Term Pancreatic Duct Ligation in the Rabbit – A Model of Non‐insulin‐dependent Diabete

“…lc). The basal plasma glucagon of 158 _ 15 ng/1 in the C group remained relatively stable over 450 days. It ranges from 120pg/ml in humans I181 and 180pg/ml in dogs I411 to 323 pg/ml and 331pg/ml in Wistar and GK rats, respectively (1).…”

Animal Models in Diabetic Research—History, Presence, and Future Perspectives

“…Another method of surgically inducing DM was described in rabbits as early as 1946 when Walpole and Innes performed a pancreatic duct ligation, which resulted in morphological changes of the islets and a slight rise in the fasting blood glucose level. Later, this model was investigated in more detail and assigned to T2DM models [21,22].…”