Cell‐free culture supernatant of
            <i>Lactobacillus curvatus</i>
            Wikim38 inhibits RANKL‐induced osteoclast differentiation and ameliorates bone loss in ovariectomized mice

Jang, Ah‐Ra; Park, Joon-Suk; Kim, Dong Kyu; Park, Jiyeon; Ahn, Jae-Hun; Kim, Dong Yeon; Lee, Tae-Sung; Chang, Ji-Yoon; Choi, Joo-Hee; Park, Jong‐Hwan

doi:10.1111/lam.13525

Cited by 11 publications

(11 citation statements)

References 29 publications

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“…A study of the effects of cell lysates and supernatants of five native probiotic strains (L. acidophilus, L. reuteri, L. casei, B. longum, and B. coagulans) in ovariectomized rats showed that the Bacillus-coagulans-derived-postbiotics had the best impact on bone homeostasis, ameliorating several parameters, such as reduced bone area, bone mineral content, and BMD caused by ovariectomy [153]. Furthermore, a corroborating study [155] using a murine model of postmenopausal-related osteoporosis reported an inhibitory effect of the postbiotic Lactobacillus curvatus 38-CS on RANKL-induced osteoclast differentiation and bone loss, by downregulating the TRAF6/NF-κB/MAPKs axis (see Figure 2 for further details).…”

Section: Interventional Microbiota Modulation To Reduce Bone Lossmentioning

confidence: 89%

“…Postbiotics is an umbrella term that includes metabolites, SCFAs, microbial cell fractions, functional proteins, extracellular polymeric substances (EPSs), cell lysates, teichoic acid, peptidoglycan-derived muropeptides, and pili-type structures, which may be used to promote health by modulating the gut microbiota [ 153 ]. In this context, only a few very recent studies [ 154 , 155 , 156 ] have shown how the administration of postbiotics ameliorates osteopenia and/or osteoporosis. A study of the effects of cell lysates and supernatants of five native probiotic strains ( L. acidophilus , L. reuteri , L. casei , B. longum , and B. coagulans ) in ovariectomized rats showed that the Bacillus -coagulans-derived-postbiotics had the best impact on bone homeostasis, ameliorating several parameters, such as reduced bone area, bone mineral content, and BMD caused by ovariectomy [ 153 ].…”

Section: Interventional Microbiota Modulation To Reduce Bone Lossmentioning

confidence: 99%

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Role of Dietary Supplements and Probiotics in Modulating Microbiota and Bone Health: The Gut-Bone Axis

Sire

Sire²,

Curci

et al. 2022

Cells

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Osteoporosis is characterized by an alteration of bone microstructure with a decreased bone mineral density, leading to the incidence of fragility fractures. Around 200 million people are affected by osteoporosis, representing a major health burden worldwide. Several factors are involved in the pathogenesis of osteoporosis. Today, altered intestinal homeostasis is being investigated as a potential additional risk factor for reduced bone health and, therefore, as a novel potential therapeutic target. The intestinal microflora influences osteoclasts’ activity by regulating the serum levels of IGF-1, while also acting on the intestinal absorption of calcium. It is therefore not surprising that gut dysbiosis impacts bone health. Microbiota alterations affect the OPG/RANKL pathway in osteoclasts, and are correlated with reduced bone strength and quality. In this context, it has been hypothesized that dietary supplements, prebiotics, and probiotics contribute to the intestinal microecological balance that is important for bone health. The aim of the present comprehensive review is to describe the state of the art on the role of dietary supplements and probiotics as therapeutic agents for bone health regulation and osteoporosis, through gut microbiota modulation.

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Section: Interventional Microbiota Modulation To Reduce Bone Lossmentioning

confidence: 89%

Section: Interventional Microbiota Modulation To Reduce Bone Lossmentioning

confidence: 99%

Role of Dietary Supplements and Probiotics in Modulating Microbiota and Bone Health: The Gut-Bone Axis

Sire

Sire²,

Curci

et al. 2022

Cells

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show abstract

“…OVX animals exhibit estrogen deficiency, and are classically used as experimental models to simulate postmenopausal bone loss and increase osteoclastic activity after ovariectomy [24][25][26][27]35]. Several studies showed that 3D Micro-CT images and histopathology assay represented significant bone loss after the OVX mice model.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of the Postbiotic Lactobacillus plantarum MD35 on Bone Loss in an Ovariectomized Mice Model

Myeong

Jung

Chae³

et al. 2023

Probiotics & Antimicro. Prot.

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Postmenopausal osteoporosis is caused by estrogen deficiency, which impairs bone homeostasis, resulting in increased osteoclastic resorption without a corresponding increase in osteoblastic activity. Postbiotics have several therapeutic properties, including anti-obesity, anti-diabetic, anti-inflammatory, and anti-osteoporotic effects. However, the beneficial effects of the postbiotic MD35 of Lactobacillus plantarum on bone have not been studied. In this study, we demonstrated that the postbiotic L. plantarum MD35, isolated from young radish water kimchi, influences osteoclast differentiation in mouse bone marrow-derived macrophage (BMM) culture. In addition, it was effective protecting against estrogen deficiency-induced bone loss in ovariectomized (OVX) mice, an animal model of postmenopausal osteoporosis. In BMM cells, postbiotic MD35 inhibited the receptor activator of nuclear factor-kappa B of NF-κB ligand (RANKL)-induced osteoclast differentiation by attenuating the phosphorylation of extracellular signal-related kinase, significantly suppressing the resorption activity and down-regulating the expression of RANKL-mediated osteoclast-related genes. In the animal model, the oral administration of postbiotic MD35 remarkably improved OVX-induced trabecular bone loss and alleviated the destruction of femoral plate growth. Therefore, postbiotic MD35 could be a potential therapeutic candidate for postmenopausal osteoporosis by suppressing osteoclastogenesis through the regulation of osteoclast-related molecular mechanisms.

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“…Further studies are needed to elucidate the precise molecular mechanism underlying the anti-osteoclast activity of the components of postbiotic MD35 on Nfatc1 expression. Recent study reported that the traditional Korean baechu kimchi could produce organic acids including lactic acid, fumaric acid, and acetic acid from kimchi cabbage waste [35,37]. Among the several acids, fumaric acid inhibits RANKL-mediated osteoclast differentiation [38], and these bioactive compounds might play a role in anti-osteoclastogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Among the several acids, fumaric acid inhibits RANKL-mediated osteoclast differentiation [38], and these bioactive compounds might play a role in anti-osteoclastogenesis. Ovariectomized (OVX) animal exhibit estrogen de ciency and are classically used as an experimental model to simulate postmenopausal bone loss and increase osteoclastic activity after ovariectomy [24][25][26][27]35]. We investigated the anti-osteoporotic effect of the postbiotic Lactobacillus plantarum MD35 in osteoporosis in vivo by comparing osteoclastic activity and BMD in the OVX model.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of the postbiotic Lactobacillus plantarum MD35 on bone loss in an ovariectomized mice model

Myeong

Jung

Chae³

et al. 2022

Preprint

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Postmenopausal osteoporosis is caused by estrogen deficiency; it impairs the homeostatic balance of the bone, resulting in bone loss owing to increased osteoclastic resorption without a corresponding increase in osteoblastic activity. Postbiotics have several pharmaceutical properties, including anti-obesity, anti-diabetic, anti-inflammatory, and anti-osteoporotic activities. However, the beneficial effects of postbiotic MD35 of Lactobacillus plantarum on bone have not been studied. In this study, we demonstrated that the postbiotic Lactobacillus plantarum MD35, isolated from young radish water kimchi influenced osteoclast differentiation in mouse bone marrow-derived macrophage (BMM) culture. In addition, it was effective in estrogen deficiency-induced bone loss in ovariectomized (OVX) mice, an animal model of postmenopausal osteoporosis. In BMM cells, postbiotic MD35 inhibited the receptor activator of nuclear factor-kappa B of NF-κB ligand (RANKL)-induced osteoclast differentiation by significantly suppressing resorption activity and downregulating the expression of RANKL-mediated osteoclast-related genes; this was achieved by attenuating the phosphorylation of extracellular signal-related kinase. In the animal model, the oral administration of postbiotic MD35 remarkably improved OVX-induced trabecular bone loss and alleviated the destruction of femoral plate growth. Therefore, postbiotic MD35 could be a potential therapeutic candidate for postmenopausal osteoporosis by suppressing osteoclastogenesis through the regulation of osteoclast-related molecular mechanisms.

show abstract

Cell‐free culture supernatant of Lactobacillus curvatus Wikim38 inhibits RANKL‐induced osteoclast differentiation and ameliorates bone loss in ovariectomized mice

Cited by 11 publications

References 29 publications

Role of Dietary Supplements and Probiotics in Modulating Microbiota and Bone Health: The Gut-Bone Axis

Role of Dietary Supplements and Probiotics in Modulating Microbiota and Bone Health: The Gut-Bone Axis

Protective Effects of the Postbiotic Lactobacillus plantarum MD35 on Bone Loss in an Ovariectomized Mice Model

Protective effects of the postbiotic Lactobacillus plantarum MD35 on bone loss in an ovariectomized mice model

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