Cell-free exosome-laden scaffolds for tissue repair

Huang, Jianghong; Xiong, Jianyi; Yang, Lei; Zhang, Jun; Sun, Shuqing; Liang, Yujie

doi:10.1039/d1nr01314a

Cited by 103 publications

(58 citation statements)

References 86 publications

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“…Extensive studies have demonstrated the role of exosomes in drug delivery (Duan et al, 2021a;Duan et al, 2021b;Duan et al, 2020b;Huang et al, 2021;Liang et al, 2021a;Xu et al, 2021). Additionally, the limitation of native exosomes in drug delivery has been recognized.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes

Ouyang¹,

XU²,

Liang³

et al. 2022

Biocell

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There is no efficient tracking system available for the therapeutic molecules delivered to cartilage. The dense matrix covering the cartilage surface is the main biological barrier that the therapeutic molecules must overcome. In this study, we aimed to establish a system that can dynamically and effectively track the therapeutic molecules delivered to cartilage. To this aim, we adopted bovine and human cartilage explants as ex vivo models for chondrocyte-targeted exosome dispersion. The efficiency of drug delivery was evaluated using frozen sections. The results of this study showed that the penetration and distribution of chondrocyte-targeted exosomes in cartilage explants can be tracked dynamically. Thus, ex vivo cartilage explants provide an effective and economic system to evaluate therapeutic drugs encapsulated in chondrocyte-targeted exosomes in preclinical studies.

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Section: Discussionmentioning

confidence: 99%

Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes

Ouyang¹,

XU²,

Liang³

et al. 2022

Biocell

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“…The 3D scaffold can be used as a platform for the release of exosomes in the joint tissue repair area [ 69 ]. Exosome-laden 3D materials can be loaded with small molecule compounds, such as miRNAs that are used for drug delivery, and used as cell-free therapeutic products for tissue repair [ 70 , 71 ].…”

Section: Discussionmentioning

confidence: 99%

3D Bioprinting of Hydrogels for Cartilage Tissue Engineering

Huang

Xiong

Wang

et al. 2021

Gels

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Three-dimensional (3D) bioprinting is an emerging technology based on 3D digital imaging technology and multi-level continuous printing. The precise positioning of biological materials, seed cells, and biological factors, known as “additive biomanufacturing”, can provide personalized therapy strategies in regenerative medicine. Over the last two decades, 3D bioprinting hydrogels have significantly advanced the field of cartilage and bone tissue engineering. This article reviews the development of 3D bioprinting and its application in cartilage tissue engineering, followed by a discussion of the current challenges and prospects for 3D bioprinting. This review presents foundational information on the future optimization of the design and manufacturing process of 3D additive biomanufacturing.

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“…This treatment method has been shown to be more beneficial than a single, direct administration of exosomes due to a slow, steady rate of delivery, with some gels delivering exosomes for up to a week [300][301][302]. These scaffolds offer structure, hydration, and increased flexibility in treatment options, as hydrogels can been used for wounds from basic skin injuries to deep nerve damage [302,303]. In the continued development of wound healing strategies continue, the broad applicability and therapeutic benefits of exosomes will only expand the prospects and efficiency of wound healing technologies.…”

Section: Inflammatorymentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

Hade

Suire

Suo

2021

Cells

275

154

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Exosomes are a type of extracellular vesicles, produced within multivesicular bodies, that are then released into the extracellular space through a merging of the multivesicular body with the plasma membrane. These vesicles are secreted by almost all cell types to aid in a vast array of cellular functions, including intercellular communication, cell differentiation and proliferation, angiogenesis, stress response, and immune signaling. This ability to contribute to several distinct processes is due to the complexity of exosomes, as they carry a multitude of signaling moieties, including proteins, lipids, cell surface receptors, enzymes, cytokines, transcription factors, and nucleic acids. The favorable biological properties of exosomes including biocompatibility, stability, low toxicity, and proficient exchange of molecular cargos make exosomes prime candidates for tissue engineering and regenerative medicine. Exploring the functions and molecular payloads of exosomes can facilitate tissue regeneration therapies and provide mechanistic insight into paracrine modulation of cellular activities. In this review, we summarize the current knowledge of exosome biogenesis, composition, and isolation methods. We also discuss emerging healing properties of exosomes and exosomal cargos, such as microRNAs, in brain injuries, cardiovascular disease, and COVID-19 amongst others. Overall, this review highlights the burgeoning roles and potential applications of exosomes in regenerative medicine.

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Cell-free exosome-laden scaffolds for tissue repair

Abstract: With the development of regenerative medicine, tissue repair at the molecular, cellular, tissue, and organ level has seen continuous improvements over traditional techniques. As the core of tissue repair, seed...

Cited by 103 publications

References 86 publications

Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes

Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes

3D Bioprinting of Hydrogels for Cartilage Tissue Engineering

Mesenchymal Stem Cell-Derived Exosomes: Applications in Regenerative Medicine

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