Cell-free layer measurements of<i>in vitro</i>blood flow in a microfluidic network: an automatic and manual approach

Bento, David; Pereira, Ana I.; Lima, José Albino; Miranda, J. M.; Lima, Rui

doi:10.1080/21681163.2017.1329029

Cited by 14 publications

(13 citation statements)

References 53 publications

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“…However, RBCs flowing in microvessels, due to the confined microenvironment, deform not only due to shear effect but also to extensional effect. Hence, from the beginning of the 21st century, and due to the progress in microfabrication [ 6 , 7 , 44 , 45 ], microflow visualization techniques [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ], and image analysis methods [ 58 , 59 , 60 , 61 , 62 , 63 ], several microfluidic devices containing microchannels have been proposed to study RBC deformability in environments closer to in vivo microcirculation. Most of the proposed microfluidic devices to perform RBC deformability characterization can be classified as fluid-induced deformation microchannels (when the dimensions of the channels used to generate deformability are larger than the tested cells) and as structure-induced deformation microchannels (constriction channels with dimensions similar or smaller than the diameter of tested cells).…”

Section: Deformation Of Rbcs In Microfluidic Devicesmentioning

confidence: 99%

Deformation of Red Blood Cells, Air Bubbles, and Droplets in Microfluidic Devices: Flow Visualizations and Measurements

et al. 2018

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Techniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access, lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels. Visualizations and measurements of the deformation of RBCs flowing through hyperbolic, smooth, and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability.

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Section: Deformation Of Rbcs In Microfluidic Devicesmentioning

confidence: 99%

Deformation of Red Blood Cells, Air Bubbles, and Droplets in Microfluidic Devices: Flow Visualizations and Measurements

et al. 2018

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“…Recently, we have performed CFL measurements in a microchannel network with a depth of about 58 µm [16]. However, in this study we have only observed one CFL at center of the channel downstream the confluences.…”

Section: Visualization and Measurement Of The Cell-free Layermentioning

confidence: 83%

Visualization and Measurement of the Cell-Free Layer (CFL) in a Microchannel Network

Bento

Fernandes

Pereira

et al. 2017

Lecture Notes in Computational Vision and Biomechanics

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Abstract. In the past years, in vitro blood studies have revealed several significant hemodynamic phenomena that have played a key role in recent developments of biomedical microdevices for cells separation, sorting and analysis. However, the blood flow phenomena happening in complex geometries, such as microchannel networks, have not been fully understood. Thus, it is important to investigate in detail the blood flow behavior occurring at microchannel networks. In the present study, by using a high-speed video microscopy system, we have used two working fluids with different haematocrit (1% Hct and 15% Hct) and we have investigated the cell-free layer (CFL) in a microchannel network composed by asymmetric bifurcations. By using the Z Project method from the image analysis software ImageJ, it was possible to conclude that the successive bifurcations and confluences influence the formation of the CFL not only along the upper and lower wall of the microchannel but also at the region immediately downstream of the confluence apex.

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“…The authors obtained, with the microdevice continuously running during 30 min without clogging, a separation efficiency of 100% for an inlet Hct of 45%, using defibrinated sheep blood, at a 10 μL•h −1 flow rate, with a yield or plasma volume percentage obtained of 15-25% [65]. During the last decade, Ishikawa et al, [67], Leble et al, [9] and Pinto et al, [23] have performed in vitro blood flow studies in simple microchannels with symmetric bifurcations and confluences and more recently Bento et al [100,101] have performed similar studies in more complex geometries such as in microchannel networks. In those works, it was observed a clear cell-depleted layer at the region of the confluence apex that can be used to perform blood plasma separation.…”

Section: Hemodynamic Phenomena On Cell Separation Techniquesmentioning

confidence: 99%

Micro/Nano Devices for Blood Analysis

Lima¹,

Minas²,

Catarino³

2019

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His research is mostly centered on the area of microfluidics, nanofluidics, and blood flow in biomedical microdevices. Currently, he lectures as part of several Master courses in Mechanical and Industrial Engineering. In the past, he has delivered lectures as part of several Master courses in Biomedical Technology, including Cardiovascular Biomechanics and Micro/Nanotechnologies and Biomedical Applications at the Bragança Polytechnic Institute (IPB). Currently, he also supervises several PhD and Master students in the field of Mechanical and Biomedical Engineering.

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Cell-free layer measurements ofin vitroblood flow in a microfluidic network: an automatic and manual approach

Cited by 14 publications

References 53 publications

Deformation of Red Blood Cells, Air Bubbles, and Droplets in Microfluidic Devices: Flow Visualizations and Measurements

Deformation of Red Blood Cells, Air Bubbles, and Droplets in Microfluidic Devices: Flow Visualizations and Measurements

Visualization and Measurement of the Cell-Free Layer (CFL) in a Microchannel Network

Micro/Nano Devices for Blood Analysis

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