Cell-Free Supernatant of L. buchneri Probiotic Bacteria Enhancing Apoptosis Activity in AGS Gastric Cancer Cells

The inhibition effects of Lentilactobacillus buchneri-derived membrane vesicles on AGS and HT-29 cancer cells by inducing cell apoptosis

“…It was identified by 16S rRNA sequencing and registered in GenBank under the accession number Lentilactobacillus sp. strain T2 (0P218100) in our previous study 33 . The safety and effectiveness of the strain were assessed according to the WHO guidelines for the evaluation of probiotic bacteria in foods.…”

“…To assess efficiency, the resistance to stomach acid and enzymes, resistance to bile salts, and the ability to attach to CaCO 2 cell lines were evaluated. This strain was only used in the subsequent tests to evaluate the anticancer effect of microvesicles after its probiotic properties were proven 33 , 48 , 49 .…”

“…The cell lines were developed in a liquid medium consisting of RPMI1640 and DMEM (Gibco, Carlsbad, USA), which was further enriched with 10% Fetal Bovine Serum (Gibco, Carlsbad, USA), 100 μ/mL penicillin (Gibco, Carlsbad, USA), and 100 μg/mL streptomycin (Gibco, Carlsbad, USA). The cells were cultivated at 37 °C in a humid environment containing 5% CO 2 33 .…”

“…Subsequently, the cells were subjected to a 30-min incubation period at ambient temperature and subsequently assessed by flow cytometry. The data underwent analysis using the FlowJo program 33 .…”

“…The experimental conditions consisted of subjecting the sample to a temperature of 95 °C for a duration of 1 min, followed by a further exposure to the same temperature of 95 °C for a period of 15 s. Finally, the sample was subjected to a temperature of 60 °C for a duration of 1 min. The relative gene expression was determined using the ΔΔCt technique, assuming a PCR efficiency of 100% 33 .…”