Cell-interactive 3D-scaffold; advances and applications

Dutta, Ranjna C.; Dutta, Aroop K.

doi:10.1016/j.biotechadv.2009.02.002

Cited by 206 publications

(133 citation statements)

References 42 publications

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“…In this respect, synthetic matrices have been developed that feature defined and tunable compositions, organization, mechanics and ECM remodeling capabilities. Indeed, in response to this need there has been literally an explosion of publications describing the generation and application of synthetic ECMs for tissue regeneration, and the reader is referred to some excellent reviews on these topics (Ayres et al, 2009;Dutta and Dutta, 2009;Lutolf and Hubbell, 2005;McCullen et al, 2009;Rosso et al, 2005;Zisch et al, 2003). One example is given by polyethylene glycol (PEG) hydrogels -frequently used biologically compatible synthetic matrices that support cell adhesion, viability and growth (Lutolf and Hubbell, 2005).…”

Section: Tumors -A Tough Situationmentioning

confidence: 99%

The extracellular matrix at a glance

Frantz

Stewart

Weaver

2010

Journal of Cell Science

3,492

2,943

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Section: Tumors -A Tough Situationmentioning

confidence: 99%

The extracellular matrix at a glance

Frantz

Stewart

Weaver

2010

Journal of Cell Science

3,492

2,943

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“…The increasing interest in tissue engineering has heightened the need for a new platform to manufacture 3D bio-printed scaffolds (Dutta and Dutta 2009). These 3D-shaped structures hold great promise for therapies aimed at tissue regeneration and organ repair and replacement (Hollister 2005;Sengers et al 2007;Mironov et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Cellulose Nanofibers for the Enhancement of Printability of Low Viscosity Gelatin Derivatives

Shin¹,

Park²,

Park³

et al. 2017

BioResources

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Inadequate rheological properties of gelatin methacrylamide (GelMA) were successfully improved by incorporating cellulose nanofibers (CNFs), such that the printed scaffolds could maintain their structural fidelity during the three-dimensional (3D) bio-printing process. The CNFs provided an outstanding shear thinning property, and the GelMA/CNF inks exhibited high zero shear viscosity and structural fidelity under a low dispensing pressure. After evaluating the printability, composite inks containing 2% w/v CNF were observed to have an optimal concentration of CNF to prepare 3D print stable constructs. Therefore, these inks were used to manufacture human nose and ear structures, producing highly porous structures in the printed composite hydrogels. Furthermore, the mechanical stability of the GelMA/CNF composite hydrogel was increased when CNFs were incorporated, which indicated that CNFs played an important role in enhancing the structural properties of the composite hydrogels. Additionally, the biocompatibility of CNF-reinforced hydrogels was evaluated using a fibroblast cell line.

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“…Threedimensional (3D) tissue model utilizing hPSC-derived neural progenitor cells (NPCs) might overcome these drawbacks in developing stable and long-term culture conditions that optimally mimic the in vivo situation [2][3][4][5]. However, 3D tissue engineering still faces major problems like cell survival and cell differentiation, which can be in part solved by the use of presynthesized scaffold [2][3][4], designed to recreate an extracellular matrix environment [6][7][8][9]. However, available scaffolds often do not support neurite outgrowth and do not allow long-term survival of cells [10][11][12].…”

mentioning

confidence: 99%

Engineering of Midbrain Organoids Containing Long-Lived Dopaminergic Neurons

TiengVannary

StoppiniLuc

VillySabrina

et al. 2014

Stem Cells and Development

103

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The possibility to generate dopaminergic (DA) neurons from pluripotent stem cells represents an unlimited source of material for tissue engineering and cell therapy for neurodegenerative disease. We set up a protocol based on the generation of size-calibrated neurospheres for a rapid production (3 weeks) of a high amount of DA neurons ( > 60%) oriented toward a midbrain-like phenotype, characterized by the expression of FOXA2, LMX1A, tyrosine hydroxylase (TH), NURR1, and EN1. By using g-secretase inhibitors and varying culture time of neurospheres, we controlled maturation and cellular composition of a three-dimensional (3D) engineered nervous tissue (ENT). ENT contained neurons and glial cells expressing various markers of maturity, such as synaptophysin, neuronal nuclei-specific protein (NeuN), and glial fibrillary acidic protein (GFAP), and were electrophysiologically active. We found that 3-week-old neurospheres were optimal to generate 3D tissue containing DA neurons with typical A9 morphology. ENT generated from 4-week-old neurospheres launched glial cell type since astrocytes and myelin could be detected massively at the expense of TH-immunoreactive neurons. All g-secretase inhibitors were not equivalent; compound E was more efficient than DAPT in generating DA neurons. This DA tissue provides a tool for drug screening, and toxicology. It should also become a useful biomaterial for studies on Parkinson's disease.

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Cell-interactive 3D-scaffold; advances and applications

Cited by 206 publications

References 42 publications

The extracellular matrix at a glance

The extracellular matrix at a glance

Cellulose Nanofibers for the Enhancement of Printability of Low Viscosity Gelatin Derivatives

Engineering of Midbrain Organoids Containing Long-Lived Dopaminergic Neurons

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