Cell-Laden Composite Hydrogel Bioinks with Human Bone Allograft Particles to Enhance Stem Cell Osteogenesis

Gharacheh, Hadis; Guvendiren, Murat

doi:10.3390/polym14183788

Cited by 10 publications

(6 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To achieve rapid gelation, self-healing post-bioprinting and subsequent stabilization through secondary crosslinking, shear-thinning, and self-healing properties, alginate was subjected to methacrylation and oxidation and then ionically crosslinked through an egg-shell model [34]. To make alginate hydrogels more suitable for bone tissue engineering, alginate hydrogels could be incorporated with inorganic particles such as bioglass [35] and kaolin [36] or with organic materials such as cells, drugs and human allograft tissue, as examples [37].…”

Section: Hydrogels Used With Calcium Phosphates In 3d Bioprintingmentioning

confidence: 99%

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Tolmacheva,

Bhattacharyya,

Noh

2024

Biomimetics

View full text Add to dashboard Cite

Three-dimensional bioprinting is a promising technology for bone tissue engineering. However, most hydrogel bioinks lack the mechanical and post-printing fidelity properties suitable for such hard tissue regeneration. To overcome these weak properties, calcium phosphates can be employed in a bioink to compensate for the lack of certain characteristics. Further, the extracellular matrix of natural bone contains this mineral, resulting in its structural robustness. Thus, calcium phosphates are necessary components of bioink for bone tissue engineering. This review paper examines different recently explored calcium phosphates, as a component of potential bioinks, for the biological, mechanical and structural properties required of 3D bioprinted scaffolds, exploring their distinctive properties that render them favorable biomaterials for bone tissue engineering. The discussion encompasses recent applications and adaptations of 3D-printed scaffolds built with calcium phosphates, delving into the scientific reasons behind the prevalence of certain types of calcium phosphates over others. Additionally, this paper elucidates their interactions with polymer hydrogels for 3D bioprinting applications. Overall, the current status of calcium phosphate/hydrogel bioinks for 3D bioprinting in bone tissue engineering has been investigated.

show abstract

Section: Hydrogels Used With Calcium Phosphates In 3d Bioprintingmentioning

confidence: 99%

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Tolmacheva,

Bhattacharyya,

Noh

2024

Biomimetics

View full text Add to dashboard Cite

show abstract

“…Undifferentiated stem cells (e.g., mesenchymal stem cells (MSCs)) have been used extensively due to their unique qualities, such as the capacity for self-renewal and ability to differentiate into multiple cell lineages. Commonly used stem cells include hASCs, [136,137] hMSCs, [138,139] and bone MSCs (bMSCs). [140,141] Recently, induced pluripotent stem cells (iPSCs) have been employed within bioprinting.…”

Section: Challenges In Fabricating Tissuementioning

confidence: 99%

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

et al. 2023

View full text Add to dashboard Cite

Advances in bioprinting have enabled the fabrication of complex tissue constructs with high speed and resolution. However, there remains significant structural and biological complexity within tissues that bioprinting is unable to recapitulate. Bone, for example, has a hierarchical organization ranging from the molecular to whole organ level. Current bioprinting techniques and the materials employed have imposed limits on the scale, speed, and resolution that can be achieved, rendering the technique unable to reproduce the structural hierarchies and cell–matrix interactions that are observed in bone. The shift towards biomimetic approaches in bone tissue engineering, where hydrogels provide biophysical and biochemical cues to encapsulated cells, is a promising approach to enhance the biological function and development of tissues for in vitro modelling. A major focus in bioprinting of bone tissue for in vitro modelling is creating the dynamic microenvironmental niches to support, stimulate, and direct the cellular processes for bone formation and remodeling. Hydrogels are ideal materials for imitating the extracellular matrix since they can be engineered to present various cues whilst allowing bioprinting. Here, we review recent advances in hydrogels and 3D bioprinting towards creating a microenvironmental niche that is conducive to tissue engineering of in vitro models of bone.This review focuses on hydrogels and 3D bioprinting in bone tissue engineering for development of in vitro models of bone. It highlights challenges in recapitulating the biological complexity seen in bone and how synergistic application of dynamic hydrogels and innovative bioprinting pipelines could address these challenges to achieve bone models.This article is protected by copyright. All rights reserved

show abstract

“…Currently, both natural and modified biomaterials are widely used in bioink design. Additionally, research on novel bioinks such as decellularized extracellular matrix bioink and nanocomposite material bioink is increasing. − In the future, by combining different materials, it will be possible to create multicomponent bioinks with excellent functionality, opening up new possibilities for their development.…”

Section: Challenges and Future Prospectsmentioning

confidence: 99%

Recent Advancements of Bioinks for 3D Bioprinting of Human Tissues and Organs

He,

Deng,

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

3D bioprinting is recognized as a promising biomanufacturing technology that enables the reproducible and high-throughput production of tissues and organs through the deposition of different bioinks. Especially, bioinks based on loaded cells allow for immediate cellularity upon printing, providing opportunities for enhanced cell differentiation for organ manufacturing and regeneration. Thus, extensive applications have been found in the field of tissue engineering. The performance of the bioinks determines the functionality of the entire printed construct throughout the bioprinting process. It is generally expected that bioinks should support the encapsulated cells to achieve their respective cellular functions and withstand normal physiological pressure exerted on the printed constructs. The bioinks should also exhibit a suitable printability for precise deposition of the constructs. These characteristics are essential for the functional development of tissues and organs in bioprinting and are often achieved through the combination of different biomaterials. In this review, we have discussed the cutting-edge outstanding performance of different bioinks for printing various human tissues and organs in recent years. We have also examined the current status of 3D bioprinting and discussed its future prospects in relieving or curing human health problems.

show abstract

Cell-Laden Composite Hydrogel Bioinks with Human Bone Allograft Particles to Enhance Stem Cell Osteogenesis

Cited by 10 publications

References 103 publications

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Calcium Phosphate Biomaterials for 3D Bioprinting in Bone Tissue Engineering

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

Recent Advancements of Bioinks for 3D Bioprinting of Human Tissues and Organs

Contact Info

Product

Resources

About