Cell Line Derived from a Murine Sarcoma Virus (Moloney Pseudotype)-Induced Tumor: Cultural, Antigenic, and Virological Properties

Massicot, Judith G.; Woods, Wilna A.; Chirigos, Michael A.

doi:10.1128/aem.22.6.1119-1122.1971

Cited by 26 publications

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“…Furthermore, we have screened media conditioned by several murine and human cell lines of lymphoid (EL-4, P815, RBL-5, RAJI, BJB, BJB-GC,) andnonlymphoid (SV40-3T3, Mc-lOT?h, MSC,) origin. As would be expected from earlier descriptions of Sarcoma Growth Factor by his colleagues (1977,1978), only the MSC cell line established from a rhabdomyosarcoma induced by murine (Moloney) sarcoma virus (Massicot et al, 1971) produced activity mitogenic for fibroblasts. For a mitogen to be operationally defined as a competence factor, three principal criteria must be satisfied.…”

Section: Discussionmentioning

confidence: 51%

Mitogenic activity elaborated by macrophage‐like cell lines acts as competence factor(s) for BALB/c 3T3 cells

Wharton

Gillespie

Russell

et al. 1982

Journal Cellular Physiology

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The culture medium from several murine macrophage-like cell lines contained a mitogenic activity that functioned synergistically with platelet-poor plasma to induce DNA synthesis in quiescent density-inhibited BALB/c 3T3 fibroblasts. This mitogenic activity was generated by P388D1 (and other established lines of) macrophage-like cells that were cultured either in medium alone or in medium supplemented with platelet-poor plasma. The amount of mitogenic activity produced was directly related to the length of time the macrophage-like cells were maintained in the medium. Serum-free medium conditioned by macrophage-cells did not stimulate DNA synthesis in density-inhibited 3T3 cells in the absence of plasma; however, a transient (4-hr) exposure to serum-free macrophage-conditioned medium allowed quiescent cells to respond to plasma-derived progression factors. The addition of plasma to 3T3 cells that had been treated with the macrophage-conditioned medium brought about DNA synthesis after a 12-hr lag. The mitogenic activity that was in macrophage-conditioned medium bound to DEAE-Sephadex and eluted in a single peak using a linear NaCl gradient. This macrophage-derived competence factor was not mitogenic for lymphocytes and was clearly separated by DEAE-Sephadex chromatography from the major peak of the previously described mitogenic monokine, Interleukin-1 (lymphocyte activating factor).

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Section: Discussionmentioning

confidence: 51%

Mitogenic activity elaborated by macrophage‐like cell lines acts as competence factor(s) for BALB/c 3T3 cells

Wharton

Gillespie

Russell

et al. 1982

Journal Cellular Physiology

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“…tumor material (Massicot et al, 1971;Plata et at., 1973). Even these examples may be the result of in vitro conditions rather than the initial transformed nature of the primary in vivo cell.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the presumptive sarcoma cells in primary MSV tumors

Becker¹,

Haskill²

1980

Intl Journal of Cancer

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Presumptive sarcoma cells have been isolated from primary MSV tumors induced in normal, immunosuppressed (ALG) and athymic nude mice. These cells were atypical in appearance, induced tumors in secondary hosts and expressed the viral antigens gp70, p30 and p12. In vitro growth characteristics of the MSV cells were tested in a variety of assays defining cell transformation. None of these indicated that the virus-positive cells were transformed. Sex chromosome marker studies were carried out to determine if secondary tumor arose solely from proliferation of donor cells or through infection of host tissue. Tumors from female irradiated mice induced by injections of tumor cells derived from male mice contained a high proportion of metaphases of non-donor origin, indicating that infection is an important but not the sole mechanism involved in tumor transfer. These data also demonstrated that many of the sarcoma cells are damaged as a result of infection as shown by the presence of chromosome breaks and aneuploid metaphases. Histologic sections of both regressor and progressor tumors showed that the virus-positive cells were scattered as single cells surrounded by inflammatory cells, rather than as masses of dividing virus-positive cells found in the MSV-induced transplanted tumor. Collectively, these data support the contention that the MSV tumor is the manifestation of a response to a highly noxious virus infection rather than a tumor of dividing, transformed malignant cells.

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“…MuSV (MuLV-M) was purified from tissueculture supernatants of the BALB/c mouse sarcoma line, MSC (Massicot et al, 1971).…”

Section: Virusesmentioning

confidence: 99%

Studies on a gross‐virus‐induced lymphoma in the rat. II. The role of cell‐membrane‐associated and serum p30 antigen in the antibody and cell‐mediated response

Knight

Mitchison

Shellam

1975

Intl Journal of Cancer

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The internal viral protein, p30-1, has been identified by cytotoxicity tests using xenogeneic sera, on MuLV-G-induced rat lymphoma cells. In this system internal virion antigens, and in particular p30, are cytotoxic target antigens for syngeneic antibody and cell-mediated cytotoxicity; p30 is also present in the serum of rats with progressively growing lymphomas, and is the principal antigen responsible for the inhibition of cell-mediated cytotoxicity produced by these sera.

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Cell Line Derived from a Murine Sarcoma Virus (Moloney Pseudotype)-Induced Tumor: Cultural, Antigenic, and Virological Properties

Abstract: A cell line derived from a murine sarcoma virus (Moloney pseudotype)-induced tumor has been established. It retains oncogenicity, releases both sarcoma and leukemia viruses, and has virus-induced cellular antigens.

Cited by 26 publications

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Mitogenic activity elaborated by macrophage‐like cell lines acts as competence factor(s) for BALB/c 3T3 cells

Mitogenic activity elaborated by macrophage‐like cell lines acts as competence factor(s) for BALB/c 3T3 cells

Characterization of the presumptive sarcoma cells in primary MSV tumors

Studies on a gross‐virus‐induced lymphoma in the rat. II. The role of cell‐membrane‐associated and serum p30 antigen in the antibody and cell‐mediated response

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