Cell-material interaction

Rychly, Joachim; Nebe, Barbara

doi:10.1515/bnm-2013-0019

Cited by 12 publications

(19 citation statements)

References 75 publications

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“…The literature postulates that physico-chemical surface characteristics such as roughness and wettability influence cell adhesion, spreading, proliferation, and differentiation [2,3,9,35]. Raimbault et al [21] pointed out that cell behavior is not necessarily positively modulated by hydrophilic surfaces, which is in accordance with our observations.…”

Section: Resultssupporting

confidence: 92%

“…The modification and optimization of the surface properties of implants with a focus on cell adhesion was investigated by many research groups [1,2,3,4]. Titanium and titanium alloys are widely used as biomaterial for implants due to their high biocompatibility [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…It is known that surface characteristics such as roughness and wettability influence cellular processes like spreading, proliferation, differentiation, and intracellular signaling via Ca 2+ ions [2,3,15]. Often a hydrophilic wetting state of surfaces is associated with an increase of bone formation in the peri-implant region in comparison to hydrophobic surfaces [4].…”

Section: Introductionmentioning

confidence: 99%

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Femtosecond Laser Nano/Micro Textured Ti6Al4V Surfaces—Effect on Wetting and MG-63 Cell Adhesion

et al. 2019

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Nano- and microstructured titanium surfaces have recently attracted attention in the field of regenerative medicine because of the influence which surface characteristics such as roughness and wettability can have on cellular processes. This study focuses on the correlation of surface properties (wettability and nano/micro texture) of laser-structured Ti6Al4V samples with pronounced cell adhesion. Samples were structured with multiple laser parameters in order to create a range of surface properties. Surface characterization was performed by contact angle measurements 1 and 7 days after laser processing. The arithmetic mean roughness of the material surface in an area (Sa) was determined by means of confocal laser scanning microscopy (CLSM). Immediately after wettability tests of the laser-structured surfaces, in vitro experiments with human MG-63 osteoblasts were carried out. For this purpose, the cell morphology and actin cytoskeleton organization were analyzed using CLSM and scanning electron microscopy. On rough microstructures with deep cavities, the cell growth and spreading were inhibited. An improved cellular adhesion and growth on nanostructured and sinusoidal microstructured surfaces could be demonstrated, regardless of hydrophilicity of the surfaces.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Femtosecond Laser Nano/Micro Textured Ti6Al4V Surfaces—Effect on Wetting and MG-63 Cell Adhesion

et al. 2019

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“…Studies indicate that cells perceive chemical and mechanical cues through chemo‐ and mechano‐sensitive molecules at the cell membrane, including integrins and stretch‐activated ion channels. That leads to focal adhesion which initiates signal transduction resulting in biological responses of the cell, such as adhesion, proliferation, differentiation, and migration . Even though such a general mechanism, the underlying molecular mechanism of mechanotransduction or chemical signaling still very much unknown.…”

Section: Discussionmentioning

confidence: 99%

Functional Nanomaterials on 2D Surfaces and in 3D Nanocomposite Hydrogels for Biomedical Applications

Tutar

Motealleh

Khademhosseini

et al. 2019

Adv Funct Materials

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An emerging approach to improve the physicobiochemical properties and the multifunctionality of biomaterials is to incorporate functional nanomaterials (NMs) onto 2D surfaces and into 3D hydrogel networks. This approach is starting to generate promising advanced functional materials such as self‐assembled monolayers (SAMs) and nanocomposite (NC) hydrogels of NMs with remarkable properties and tailored functionalities that are beneficial for a variety of biomedical applications, including tissue engineering, drug delivery, and developing biosensors. A wide range of NMs, such as carbon‐, metal‐, and silica‐based NMs, can be integrated into 2D and 3D biomaterial formulations due to their unique characteristics, such as magnetic properties, electrical properties, stimuli responsiveness, hydrophobicity/hydrophilicity, and chemical composition. The highly ordered nano‐ or microscale assemblies of NMs on surfaces alter the original properties of the NMs and add enhanced and/or synergetic and novel features to the final SAMs of the NM constructs. Furthermore, the incorporation of NMs into polymeric hydrogel networks reinforces the (soft) polymer matrix such that the formed NC hydrogels show extraordinary mechanical properties with superior biological properties.

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“…The surfaces of biomaterials play a central role in the modulation of cell behavior [42,43] and in mechanotransduction [7,44,45]. Topographical features have an influence on direct transmission of force, which causes a reorganization of the cytoskeleton [31,[46][47][48] and changes in cell shape to adapt to the underlying surface [44,49,50].…”

Section: Discussionmentioning

confidence: 99%

ROS Dependent Wnt/β-Catenin Pathway and Its Regulation on Defined Micro-Pillars—A Combined In Vitro and In Silico Study

Staehlke

Haack

Waldner

et al. 2020

Cells

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The physico-chemical surface design of implants influences the surrounding cells. Osteoblasts on sharp-edged micro-topographies revealed an impaired cell phenotype, function and Ca2+ mobilization. The influence of edges and ridges on the Wnt/β-catenin pathway in combination with the cells’ stress response has not been clear. Therefore, MG-63 osteoblasts were studied on defined titanium-coated micro-pillars (5 × 5 × 5 µm) in vitro and in silico. MG-63s on micro-pillars indicated an activated state of the Wnt/β-catenin pathway. The β-catenin protein accumulated in the cytosol and translocated into the nucleus. Gene profiling indicated an antagonism mechanism of the transcriptional activity of β-catenin due to an increased expression of inhibitors like ICAT (inhibitor of β-catenin and transcription factor-4). Cells on pillars produced a significant reactive oxygen species (ROS) amount after 1 and 24 h. In silico analyses provided a detailed view on how transcriptional activity of Wnt signaling is coordinated in response to the oxidative stress induced by the micro-topography. Based on a coordinated expression of regulatory elements of the Wnt/β-catenin pathway, MG-63s are able to cope with an increased accumulation of β-catenin on micro-pillars and suppress an unintended target gene expression. Further, β-catenin may be diverted into other signaling pathways to support defense mechanisms against ROS.

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Cell-material interaction

Cited by 12 publications

References 75 publications

Femtosecond Laser Nano/Micro Textured Ti6Al4V Surfaces—Effect on Wetting and MG-63 Cell Adhesion

Femtosecond Laser Nano/Micro Textured Ti6Al4V Surfaces—Effect on Wetting and MG-63 Cell Adhesion

Functional Nanomaterials on 2D Surfaces and in 3D Nanocomposite Hydrogels for Biomedical Applications

ROS Dependent Wnt/β-Catenin Pathway and Its Regulation on Defined Micro-Pillars—A Combined In Vitro and In Silico Study

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