Cell-mediated lympholysis by fetal and neonatal lymphocytes in sheep and man

Granberg, Christer; Hirvonen, Toivo

doi:10.1016/0008-8749(80)90233-6

Cited by 49 publications

(35 citation statements)

References 29 publications

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“…Fetal lamb MNL proliferated in response to alloantigens, presented as stimulator cells from unrelated adults. This confirms the observations of Granberg and Hirvonen (14). Comparable MLC responses are seen in the human fetus during this period in gestation (24).…”

Section: (23)supporting

confidence: 91%

“…Studies are under way to explore these possibilities. The decrease in the number of these cells in the fetus may be related to the marked impairment in the generation of all-specific cytotoxic cells during MLC responses in the fetal lamb (14). It may also be related to the deficiency in lymphokine synthesis that we demonstrated.…”

Section: (23)mentioning

confidence: 61%

“…The absence of an easily identifiable T cell marker in sheep has hampered the analysis of results from intrauterine immune manipulations, such as thymectomy in this animal model (9,10). Previous studies in ovine and other species suggest development of the afferent arm of the immune response represented by proliferative responses before development of the efferent arm represented by effector cells (14,29). The ontogeny of lymphokine production has not been evaluated in this animal model.…”

mentioning

confidence: 90%

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Ontogeny of the Immune System: Fetal Lamb as a Model

et al. 1984

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Section: (23)supporting

confidence: 91%

Section: (23)mentioning

confidence: 61%

mentioning

confidence: 90%

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Ontogeny of the Immune System: Fetal Lamb as a Model

et al. 1984

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“…Ontogeny of some phagocytic functions, but not chemotaxis, has been evaluated in fetal and neonatal animal models (6,7). The ovine fetus has been shown by us and other investigators to be an appropriate animal model to study the ontogeny of various biological phenomena (8,9). In this report, we describe the developmental sequence of neutrophil chemotaxis in fetal lambs, utilizing EAP as a source of complement-derived chemotactic factor.…”

mentioning

confidence: 94%

Ontogeny of Neutrophil Chemotaxis in Fetal Lambs

et al. 1986

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ABSTRAfl. Ontogeny of neutrophil chemotactic response using endotoxin activated adult sheep plasma as a source of complement derived chemotactic factor was examined in fetal lambs of gestational age 120-150 days. (Gestational period in sheep is approximately 150 days.) Neutrophils from fetal lambs of gestational age 120-130 days failed to respond to this chemotactic factor whereas neutrophils from fetal lambs above 131 days of gestational age responded at levels comparable to adult values. Examination of neutrophil chemotaxis in older fetuses using a different chemotactic factor derived from mitogen stimulated adult mononuclear cells revealed a selective failure of fetal neutrophils to respond to lymphocyte-derived chemotactic factor in the presence of a normal response to complement derived chemotactic factor. Among prematurely delivered twin fetuses alterations in comparison with the first twin or age-matched controls in peripheral neutrophi1 count (increase) and in chemotaxis (increase or decrease) were noted in second of twins delivered r 20 min after the first lamb, suggesting an extreme sensitivity of neutrophil functions to a variety of influences, similar to that seen in humans. Directed migration or chemotaxis of phagocytes induced by various chemotactic agents is an essential component of inflammatory response. Defects in chemotaxis are associated, as a clinical consequence, with an increased susceptibility to infections. Defects in phagocyte chemotaxis may be due to membrane defects, reduced number of receptors for the chemotactic agent, or to cytoskeletal abnormalities. The chemotactic defect of human neonatal phagocyte originally described by Miller (1) may be due to deficiencies in any or all these components (2-5). Ontogeny of some phagocytic functions, but not chemotaxis, has been evaluated in fetal and neonatal animal models (6, 7). The ovine fetus has been shown by us and other investigators to be an appropriate animal model to study the ontogeny of various biological phenomena (8,9). In this report, we describe the developmental sequence of neutrophil chemotaxis in fetal lambs, utilizing EAP as a source of complement-derived chemotactic factor. Results from simultaneous evaluation of neutrophil chemotactic responses to EAP and another chemotactic factor derived from mitogen-stimulated adult ovine mononuclear leukocyte (N-LDCF) in a limited number of fetuses are presented. In addition, neutrophil counts and chemotaxis in normal fetal lambs and their twins have been compared to document alterations that may be due to "stress" of delayed delivery. MATERIALS AND METHODSSample collection. Normal adult sheep were used as controls.Heparinized (10 U/ml) blood was collected from the external jugular vein. Lambs of gestational age 120-1 50 days and postnatal age 1-6 days were compared with adults. The gestational age was determined by time-dated breeding. The fetal lambs were normal and were being delivered prematurely for other studies. The fetuses were exposed by hysterotomy under maternal spinal anes...

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“…Cell-mediated Iympholysis (CML) has becn described in peripheral blood of human fetuses of 18-2 1 wk gestation. but no such activity was detected in fetal thymus or spleen cells (2)(3)(4). No studies have been published that specifically address the issue of ADCC during fetal life.…”

mentioning

confidence: 98%