Cell-membrane coated iron oxide nanoparticles for isolation and specific identification of drug leads from complex matrices

Abstract

New drug discovery technique to identify compounds binding to transmembrane receptors in complex mixtures.

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

“…This has been mainly attributed to their involvement in membrane destabilization of phagosomes and in facilitating the release of bacteria into the cytosol of the host cells [ 241 ]. For instance, Sherwood and colleagues used IONs coated with binding compounds of bacterial membranes for the development of drug delivery systems with endosomal escape abilities, which can be potentially used for the delivery of pharmacological cargoes [ 267 ]. Similarly, in previous work by our group, we developed novel cell-penetrating nanobioconjugates by the immobilization of the outer membrane protein A (OmpA) of Escherichia coli on IONs.…”

“…Hybrid ION/UCNP systems have been widely applied in cancer therapy, MRI and diagnosis, gene therapy, and drug delivery [ 308 ]. In general, the most prevalent host matrices for iron oxide core-shell nanoparticles include Y 2 O 3 , Y 2 O 2 S, LaF 3 , BaYF 5 , NaYF 4 , and NaGdF 4 , which have been doped with Yb 3+ /Tm 3+ and Yb 3+ /Er 3+ ions [ 230 , 231 , 232 , 233 , 234 , 235 , 236 , 237 , 238 , 239 , 240 , 241 , 242 , 243 , 244 , 245 , 246 , 247 , 248 , 249 , 250 , 251 , 252 , 253 , 254 , 255 , 256 , 257 , 258 , 259 , 260 , 261 , 262 , 263 , 264 , 265 , 266 , 267 , 268 , 269 , …”

Therapeutic lipid-coated hybrid nanoparticles against bacterial infections

“…Consistent with the idea of fusing nanoparticles with lipid vesicles to form a core-shell structure, another category of LCHNPs using cellular membranes as the lipid coating, has recently also generated interest. [62][63][64][65][66] Lipids from cellular membranes constitute 50% of the mass, with phospholipids being the most abundant. 67 Membrane lipids typically form bilayers, complexed with membrane proteins and other components.…”

Preparation and Application of Iron Oxide Nanoclusters

“…Most recently, we [42] and others [43,44] have developed a new type of magnetic separation method based on cell membrane-encapsulated iron oxide nanoclusters. Compared to traditional magnetic separation techniques using immobilized ligands on nanocluster surfaces to bind the targets, the new technique uses functional transmembrane receptors as binding sites to identify the targets.…”

“…Cell membrane-encapsulated iron oxide nanoclusters: (a) design concept, (b) TEM image, (c) HPLC washing and elution chromatograms of fishing experiments using α 3 β 4 receptors from smoke condensates, (d) comparison of elution profiles with and without α 3 β 4 receptors (adapted from Reference[42]; reproduced with permission from the Royal Society of Chemistry).…”