Cell membrane engineering with synthetic materials: Applications in cell spheroids, cellular glues and microtissue formation

Amaral, A.P.; Pasparakis, George

doi:10.1016/j.actbio.2019.04.013

Cited by 35 publications

(30 citation statements)

References 181 publications

(124 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another method to modulate the assembly of cells involves chemical modification of cell membranes, and various types of 3D tissues have been developed by these approaches. [111][112][113] In particular, chemical modification of cell membranes enabled quicker production spheroids relative to existing methods, and allowed cells, which had difficulty in spontaneous aggregate formation, to be successfully harvested as spheroids. For example, membranes of HL-60 cells, which are intrinsically difficult to form into aggregates, were functionalized with PNIPAAm through sialic acid biosynthesis followed by a thiol-ene reaction, and it was confirmed that HL-60 aggregation can be controlled by temperature change due to the phase separation property of PNIPAAm.…”

Section: Modification Of Cell Membranesmentioning

confidence: 99%

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

Kim

Yamamoto

et al. 2020

Adv Healthcare Materials

148

104

View full text Add to dashboard Cite

Multi-cellular spheroids are formed as a 3D structure with dense cell-cell/cell-extracellular matrix interactions, and thus, have been widely utilized as implantable therapeutics and various ex vivo tissue models in tissue engineering. In principle, spheroid culture methods maximize cell-cell cohesion and induce spontaneous cellular assembly while minimizing cellular interactions with substrates by using physical forces such as gravitational or centrifugal forces, protein-repellant biomaterials, and micro-structured surfaces. In addition, biofunctional materials including magnetic nanoparticles, polymer microspheres, and nanofiber particles are combined with cells to harvest composite spheroids, to accelerate spheroid formation, to increase the mechanical properties and viability of spheroids, and to direct differentiation of stem cells into desirable cell types. Biocompatible hydrogels are developed to produce microgels for the fabrication of size-controlled spheroids with high efficiency. Recently, spheroids have been further engineered to fabricate structurally and functionally reliable in vitro artificial 3D tissues of the desired shape with enhanced specific biological functions. This paper reviews the overall characteristics of spheroids and general/advanced spheroid culture techniques. Significant roles of functional biomaterials in advanced spheroid engineering with emphasis on the use of spheroids in the reconstruction of artificial 3D tissue for tissue engineering are also thoroughly discussed.

show abstract

Section: Modification Of Cell Membranesmentioning

confidence: 99%

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

Kim

Yamamoto

et al. 2020

Adv Healthcare Materials

148

104

View full text Add to dashboard Cite

show abstract

“…The size and shape of the initial cell aggregates are important starting conditions for organoids formation. Microwell structures or microfluidic devices usually achieve controlled cell aggregation; alternatively, the surface of cells, the cell membrane, can be modified to improve or initiate cell clustering [98]. There are different strategies for controlling spatial cell arrangement in vitro, from binding peptides proteins, nanoparticles, polymers, or bio-orthogonal chemical species [99,100], to cell surface binding of 3D DNA origami nanostructure that enables the programming of cell-cell adhesion [101].…”

Section: Organoid Generationmentioning

confidence: 99%

From Spheroids to Organoids: The Next Generation of Model Systems of Human Cardiac Regeneration in a Dish

Scalise

Marino

Salerno

et al. 2021

IJMS

View full text Add to dashboard Cite

Organoids are tiny, self-organized, three-dimensional tissue cultures that are derived from the differentiation of stem cells. The growing interest in the use of organoids arises from their ability to mimic the biology and physiology of specific tissue structures in vitro. Organoids indeed represent promising systems for the in vitro modeling of tissue morphogenesis and organogenesis, regenerative medicine and tissue engineering, drug therapy testing, toxicology screening, and disease modeling. Although 2D cell cultures have been used for more than 50 years, even for their simplicity and low-cost maintenance, recent years have witnessed a steep rise in the availability of organoid model systems. Exploiting the ability of cells to re-aggregate and reconstruct the original architecture of an organ makes it possible to overcome many limitations of 2D cell culture systems. In vitro replication of the cellular micro-environment of a specific tissue leads to reproducing the molecular, biochemical, and biomechanical mechanisms that directly influence cell behavior and fate within that specific tissue. Lineage-specific self-organizing organoids have now been generated for many organs. Currently, growing cardiac organoid (cardioids) from pluripotent stem cells and cardiac stem/progenitor cells remains an open challenge due to the complexity of the spreading, differentiation, and migration of cardiac muscle and vascular layers. Here, we summarize the evolution of biological model systems from the generation of 2D spheroids to 3D organoids by focusing on the generation of cardioids based on the currently available laboratory technologies and outline their high potential for cardiovascular research.

show abstract

“…Using the analogy of a hypothetical biomimetic material we realize that man-made materials still lag far behind its biological counterparts. It is encouraging, however, that this opportunity has been recognized, nucleating a rapidly growing research field ( Amaral and Pasparakis, 2019 ; Chen and Li, 2020 ). Future advancements into bioinspired and biomimetic material will likely benefit from computational approaches ( Sun et al, 2018 ; Schroer et al, 2020 ), which allow rapid advancement of theoretical and experimental data of membrane-MCA-cortex dynamics at an ever-increasing spatio-temporal resolution.…”

Section: Discussionmentioning

confidence: 99%

Cellular Membranes, a Versatile Adaptive Composite Material

Lamparter

Galic

2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Cellular membranes belong to the most vital yet least understood biomaterials of live matter. For instance, its biomechanical requirements substantially vary across species and subcellular sites, raising the question how membranes manage to adjust to such dramatic changes. Central to its adaptability at the cell surface is the interplay between the plasma membrane and the adjacent cell cortex, forming an adaptive composite material that dynamically adjusts its mechanical properties. Using a hypothetical composite material, we identify core challenges, and discuss how cellular membranes solved these tasks. We further muse how pathological changes in material properties affect membrane mechanics and cell function, before closing with open questions and future challenges arising when studying cellular membranes.

show abstract

Cell membrane engineering with synthetic materials: Applications in cell spheroids, cellular glues and microtissue formation

Cited by 35 publications

References 181 publications

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

From Spheroids to Organoids: The Next Generation of Model Systems of Human Cardiac Regeneration in a Dish

Cellular Membranes, a Versatile Adaptive Composite Material

Contact Info

Product

Resources

About