Cell-modified bioprinted microspheres for vascular regeneration

Shen, Jian; Ji, Yongli; Xie, Mingjun; Zhao, Huijie; Wang, Xuan; Li, Yin; Xu, Fangfang; Su, Sheng-an; Nie, Jing; Xie, Yao; Gao, Qing; Ma, Hong; Ke, Xueying; Shi, Zhenyu; Fu, Jianzhong; Liu, Zhenjie; He, Yong; Xiang, Meixiang

doi:10.1016/j.msec.2020.110896

Cited by 14 publications

(10 citation statements)

References 43 publications

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“…All three markers were found to be involved in either cell proliferation directly or to acts via the activation of downstream signals. Ki67 is a nuclear protein that is involved in cell proliferation and is also known to be an excellent marker for predicting the growth fraction of a specific cell population [48]. Therefore, it could be seen that Si1.0 was able to enhance cellular proliferation, which was in accordance with the proliferation results discussed above.…”

Section: Protein Expression Of Hdf On Si-gelma Hydrogelsupporting

confidence: 81%

Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation

Lin

Lee

et al. 2020

Polymers

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Wound healing is a complex process that requires specific interactions between multiple cells such as fibroblasts, mesenchymal, endothelial, and neural stem cells. Recent studies have shown that calcium silicate (CS)-based biomaterials can enhance the secretion of growth factors from fibroblasts, which further increased wound healing and skin regeneration. In addition, gelatin methacrylate (GelMa) is a compatible biomaterial that is commonly used in tissue engineering. However, it has low mechanical properties, thus restricting its fullest potential for clinical applications. In this study, we infused Si ions into GelMa hydrogel and assessed for its feasibility for skin regeneration applications by observing for its influences on human dermal fibroblasts (hDF). Initial studies showed that Si could be successfully incorporated into GelMa, and printability was not affected. The degradability of Si-GelMa was approximately 20% slower than GelMa hydrogels, thus allowing for better wound healing and regeneration. Furthermore, Si-GelMa enhanced cellular adhesion and proliferation, therefore leading to the increased secretion of collagen I other important extracellular matrix (ECM) remodeling-related proteins including Ki67, MMP9, and decorin. This study showed that the Si-GelMa hydrogels were able to enhance the activity of hDF due to the gradual release of Si ions, thus making it a potential candidate for future skin regeneration clinical applications.

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Section: Protein Expression Of Hdf On Si-gelma Hydrogelsupporting

confidence: 81%

Calcium Silicate-Activated Gelatin Methacrylate Hydrogel for Accelerating Human Dermal Fibroblast Proliferation and Differentiation

Lin

Lee

et al. 2020

Polymers

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“…live/dead staining), flow cytometry (FCM), and so on [ 167 – 170 ]. And the specificity of artificial vessels can be evaluated by conducting fluorescent stainings, ELISA or PCR [ 171 – 173 ]. Specially, the involved live/dead staining and fluorescent staining can be visualized under a light/electron or a multiphoton microscope.…”

Section: Transformation Of Vascular Scaffolds To Clinical Productivitmentioning

confidence: 99%

3D printing of tissue engineering scaffolds: a focus on vascular regeneration

et al. 2021

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“…1 A) Schematic experimental setup of the electrostatic droplet extrusion method [ 18 ]. B) Generation process of alginate microspheres with HEK293T cells, including experimental setup and droplet-dispensing system [ 29 ]. …”

Section: Encapsulation Technologies For Angiogenic Therapymentioning

confidence: 99%

“…The ability of genetically modified cells ( ex vivo gene therapy) to promote angiogenesis in a murine hindlimb ischemia model has also been explored [ 28 , 29 ]. Alginate microbeads produced by electrostatic extrusion containing HEK293T cells with stably integrated VEGF-a plasmids ( Fig.…”

Section: Encapsulation Technologies For Angiogenic Therapymentioning

confidence: 99%

“…On day 7, microbeads stably overexpressing VEGF promoted muscle regeneration and reduced apoptosis and toe necrosis. In addition, capillary densities and perfusion were increased in the mice that received these microbeads compared with those that received microbeads containing scramble-transfected HEK293T cells [ 29 ]. VEGF-secreting human umbilical vein endothelial cell (HUVEC)-microbeads were produced by electrospraying the methacrylate gelatin (GelMA) and alginate mixture followed by secondary crosslinking of GelMA with UV light and alginate hydrogel chelation using sodium citrate solution.…”

Section: Encapsulation Technologies For Angiogenic Therapymentioning

confidence: 99%

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