Cell Movement Patterns during Gastrulation in the Chick Are Controlled by Positive and Negative Chemotaxis Mediated by FGF4 and FGF8

Yang, Xuesong; Dormann, Dirk; Münsterberg, Andrea; Weijer, Cornelis J.

doi:10.1016/s1534-5807(02)00256-3

Cited by 310 publications

(338 citation statements)

References 58 publications

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“…FGF4 and FGF8 are required during mouse gastrulation for specification of endodermal and mesodermal derivatives and cell migration through the primitive streak (Amaya et al 1993;Isaacs et al 1994;Schulte-Merker and Smith 1995). These FGFs also specify mesodermal fates and regulate gastrulation movements in the frog, Xenopus (Isaacs et al 1994), zebrafish (Griffin et al 1995), and chick (Yang et al 2002). Signaling through FGFR1 orchestrates epithelial-mesenchymal transformation (EMT) during mouse gastrulation by activating a genetic network, which includes snail and E-cadherin genes (Ciruna and Rossant 2001).…”

Section: Conserved Roles Of Fgf Signalingmentioning

confidence: 99%

FGF signaling in gastrulation and neural development in Nematostella vectensis, an anthozoan cnidarian

2007

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The fibroblast growth factor (FGF) signal transduction pathway serves as one of the key regulators of early metazoan development, displaying conserved roles in the specification of endodermal, mesodermal, and neural fates during vertebrate development. FGF signals also regulate gastrulation, in part, by triggering epithelial to mesenchymal transitions in embryos of both vertebrates and invertebrates. Thus, FGF signals coordinate gastrulation movements across many different phyla. To help understand the breadth of FGF signaling deployment across the animal kingdom, we have examined the presence and expression of genes encoding FGF pathway components in the anthozoan cnidarian Nematostella vectensis. We isolated three FGF ligands (NvFGF8A, NvFGF8B, and NvFGF1A), two FGF receptors (NvFGFRa and NvFGFRb), and two orthologs of vertebrate FGF responsive genes, Sprouty (NvSprouty), an inhibitor of FGF signaling, and Churchill (NvChurchill), a Zn finger transcription factor. We found these FGF ligands, receptors, and response gene expressed asymmetrically along the oral/aboral axis during gastrulation and in a developing chemosensory structure of planula stages known as the apical tuft. These results suggest a conserved role for FGF signaling molecules in coordinating both gastrulation and neural induction that predates the Cambrian explosion and the origins of the Bilateria.

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Section: Conserved Roles Of Fgf Signalingmentioning

confidence: 99%

FGF signaling in gastrulation and neural development in Nematostella vectensis, an anthozoan cnidarian

2007

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“…Soon after this, the paths of cells become biased toward the organizer with the strongest effect on cells closest to the organizer. In chick, FGF8 is expressed in the primitive streak and functions as a chemorepellent to regulate migration of mesodermal cells away from the primitive streak, while FGF4 is expressed in Hensen's node, the chick equivalent of the organizer, and has attractant activity (Yang et al, 2002). It is unclear whether FGFs serve a similar role in zebrafish.…”

Section: How Many Directional Influences and Their Sources?mentioning

confidence: 99%

Initiation of convergence and extension movements of lateral mesoderm during zebrafish gastrulation

Sepich

Calmelet

Kiskowski

et al. 2005

Developmental Dynamics

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Embryonic morphogenesis is accomplished by cellular movements, rearrangements, and cell fate inductions. Vertebrate gastrulation entails morphogenetic processes that generate three germ layers, endoderm, mesoderm, and ectoderm, shaped into head, trunk, and tail. To understand how cell migration mechanistically contributes to tissue shaping during gastrulation, we examined migration of lateral mesoderm in the zebrafish. Our results illustrate that cell behaviors, different from mediolaterally oriented cell intercalation, also promote convergence and extension (C&E). During early gastrulation, upon internalization, individually migrating mesendodermal cells contribute to the elongation of the mesoderm by moving animally, without dorsal movement. Convergence toward dorsal starts later, by 70% epiboly (7.7 hpf). Depending on location along the Animal-Vegetal axis, an animal or vegetal bias is added to the dorsalward movement, so that paths fan out and the lateral mesoderm both converges and extends. Onset of convergence is independent of noncanonical Wnt signaling but is delayed when Stat3 signaling is compromised. To understand which aspects of motility are controlled by guidance cues, we measured turning behavior of lateral mesodermal cells. We show that cells exhibit directional preference, directionally-regulated speed, and turn toward dorsal when off-course. We estimate that ectoderm could supply from a fraction to all the dorsalward displacement seen in mesoderm cells. Using mathematical modeling, we demonstrate that directional preference is sufficient to account for mesoderm convergence and extension, and that, at minimum, two sources of guidance cues could orient cell paths realistically if located in the dorsal midline. Developmental Dynamics 234:279 -292, 2005.

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“…Fibroblast-growth-factor receptors (FGFR) have been implicated in early steps of directional cell migration in development (Szebenyi and Fallon, 1999). One function of FGFRs in vivo is to recognize chemotactic gradients of FGF that direct cell migration in the embryo (Sato and Kornberg, 2002;Sutherland et al, 1996;Yang et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

The RhoGEF Pebble is required for cell shape changes during cell migration triggered by theDrosophilaFGF receptor Heartless

et al. 2004

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The FGF receptor Heartless (HTL) is required for mesodermal cell migration in the Drosophila gastrula. We show that mesoderm cells undergo different phases of specific cell shape changes during mesoderm migration. During the migratory phase, the cells adhere to the basal surface of the ectoderm and exhibit extensive protrusive activity. HTL is required for the protrusive activity of the mesoderm cells. Moreover, the early phenotype of htl mutants suggests that HTL is required for the adhesion of mesoderm cells to the ectoderm.In a genetic screen we identified pebble (pbl) as a novel gene required for mesoderm migration. pbl encodes a guanyl nucleotide exchange factor (GEF) for RHO1 and is known as an essential regulator of cytokinesis. We show that the function of PBL in cell migration is independent of the function of PBL in cytokinesis. Although RHO1 acts as a substrate for PBL in cytokinesis, compromising RHO1 function in the mesoderm does not block cell migration. These data suggest that the function of PBL in cell migration might be mediated through a pathway distinct from RHO1. This idea is supported by allele-specific differences in the expressivity of the cytokinesis and cell migration phenotypes of different pbl mutants. We show that PBL is autonomously required in the mesoderm for cell migration. Like HTL, PBL is required for early cell shape changes during mesoderm migration. Expression of a constitutively active form of HTL is unable to rescue the early cellular defects in pbl mutants, suggesting that PBL is required for the ability of HTL to trigger these cell shape changes. These results provide evidence for a novel function of the Rho-GEF PBL in HTL-dependent mesodermal cell migration.

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Cell Movement Patterns during Gastrulation in the Chick Are Controlled by Positive and Negative Chemotaxis Mediated by FGF4 and FGF8

Cited by 310 publications

References 58 publications

FGF signaling in gastrulation and neural development in Nematostella vectensis, an anthozoan cnidarian

FGF signaling in gastrulation and neural development in Nematostella vectensis, an anthozoan cnidarian

Initiation of convergence and extension movements of lateral mesoderm during zebrafish gastrulation

The RhoGEF Pebble is required for cell shape changes during cell migration triggered by theDrosophilaFGF receptor Heartless

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