Cell Nucleus Penetration by Quantum Dots Induced by Nuclear Staining Organic Fluorophore and UV‐Irradiation

Xu, Youlong; Wang, Qiangbin; He, Ping; Dong, Qiao‐Mei; Liu, Fang; Liu, Yan; Lin, Lin; Yan, Hao; Zhao, Xia

doi:10.1002/adma.200703238

Cited by 18 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The penetration of quantum dots into the cytoplasm has been widely investigated, but there have been few studies on penetration into the nucleus. Interestingly, in contrast to most unmodified CQDs, the as‐prepared N‐CQDs could penetrate into the nucleus without any further modification; this is beneficial to simplify the production procedure and save costs. This behavior would be useful in cell nucleus labeling.…”

Section: Introductionsupporting

confidence: 87%

One‐Pot Green Synthesis of Nitrogen‐Doped Carbon Quantum Dots for Cell Nucleus Labeling and Copper(II) Detection

Tian

Kuga

et al. 2017

Chemistry An Asian Journal

View full text Add to dashboard Cite

The doping of nitrogen into carbon quantum dots is vitally important for improved fluorescence performance. However, the synthesis of nitrogen-doped carbon quantum dots (N-CQDs) is usually conducted under strong acid and high temperature, which results in environmental pollution and energy consumption. Herein, the N-CQDs were prepared by a mild one-pot hydrothermal process. The hydrothermal reaction temperature was adjusted to control the particle size, nitrogen/carbon atomic ratio, and quantum yield. The products were water soluble with a narrow particle size distribution and good dispersion stability over a wide pH range. The N-CQDs could penetrate into the HeLa cell nucleus without any further functionalization. Moreover, the fluorescence of N-CQDs could be selectively quenched by Cu , which suggested applications for the detection of Cu in human plasma.

show abstract

Section: Introductionsupporting

confidence: 87%

One‐Pot Green Synthesis of Nitrogen‐Doped Carbon Quantum Dots for Cell Nucleus Labeling and Copper(II) Detection

Tian

Kuga

et al. 2017

Chemistry An Asian Journal

View full text Add to dashboard Cite

show abstract

“…Another previous work of ours revealed that QDs cause chronic toxicity to reproduction and development of the next generation, which might be related to QD‐caused DNA damage 26. It has been suggested that QDs might penetrate the nuclear membrane and contact DNA directly 19. Yang et al reported that Chinese hamster ovary K1 (CHO K1) cells treated with cadmium were blocked at the G2/M and G1/S phase 29.…”

Section: Resultsmentioning

confidence: 88%

“…A number of studies have determined the cellular uptake mechanisms and intracellular location of QDs 16–18. Xu et al even found that green 3‐mercaptopropionic acid (MPA)‐modified QDs could translocate from the cytoplasm to the nucleus, but the red MPA–QDs, PEG–QDs, and antibody‐conjugated QDs stayed in the cytoplasm 19. These findings indicated that certain QDs might penetrate the nuclear membrane and contact DNA directly, which might subsequently contribute to disturbing the cell cycle and the process of cell division.…”

Section: Introductionmentioning

confidence: 99%

The Influence on Cell Cycle and Cell Division by Various Cadmium‐Containing Quantum Dots

Liu

Wang

et al. 2013

Small

View full text Add to dashboard Cite

Quantum dots (QDs) have attracted great attention because of their favorable optical properties and have been widely applied in biomedical fields. However, in recent years, there have been an increasing number of reports about the cytotoxicity of QDs, especially cadmium-containing QDs, which may release cadmium ions to induce cytotoxicity. Importantly, the chemical composition and surface modifications of cadmium-based QDs determine the amount of Cd(2+) released inside the cell. Thus, there is an urgent need for more systematic work to study the relationship between cytotoxicity and the surface properties of QDs. In this article, the cytotoxicity of seven cadmium-containing QDs with different constituent elements and surface chemistries are compared. The results show that the cytotoxicity of QDs is closely related to their constituent elements and surface properties: First, CdTe@ZnS core-shell QDs show much lower cytotoxicity than naked ones when they have similar surface modifications; second, the positively charged QDs are more toxic than the negatively charged ones. Moreover, both positively and negatively charged QDs without ZnS coatings lead to multipolar spindles, misaligned chromosomes, and G2/M checkpoint failures. Interestingly, although CdSe QDs with a PEG coating cause no apparent cytotoxicity in any of the cell lines studied, they can localize near the contractile ring during cytokinesis and then block contractile ring disassembly. The cellular effect of CdTe QDs comes not only from the release of cadmium ions but also the intracellular distribution of QD nanoparticles in cells and the associated nanoscale effects. It is also found that QD-caused cytokinesis failure is closely related to the decreased expression of Cyclin A and Cyclin B. Taken together, the above findings provide new insight into the dynamic fate of QDs during cell mitosis, and are important for understanding the intracellular effects of QDs on the mitotic spindle and chromosomes during cell division. Furthermore, this kind of cytotoxicity evaluation method should be applicable to studies of the biological effects and health impacts of other nanomaterials.

show abstract

“…214 Active transportation is characterized by ligand-receptormediated transportation using ligands, such as transferrin (Tf) 215 or antibodies. 66,[216][217][218][219] A very popular approach is the use of cellpenetrating peptides (CPPs). 220 The peptide sequences, generally referred to as protein transduction domains, often include short segments from the human immunodeficiency virus 1 transcriptional activator Tat protein (HIV-1 Tat) and homopolymers of arginine.…”

Section: Cell Deliverymentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

View full text Add to dashboard Cite

Semiconductor quantum dots (QDs) have become important fluorescent probes for in vitro and in vivo bioimaging research. Their nanoparticle surfaces for versatile bioconjugation, their adaptable photophysical properties for multiplexed detection, and their superior stability for longer investigation times are the main advantages of QDs compared to other fluorescence imaging agents. Here, we review the recent literature dealing with the design and application of QD-bioconjugates for advanced in vitro and in vivo imaging. After a short summary of QD preparation and their most important properties, different QD-based imaging applications will be discussed from the technological and the biological point of view, ranging from super-resolution microscopy and single-particle tracking over in vitro cell and tissue imaging to in vivo investigations. A substantial part of the review will focus on multifunctional applications, in which the QD fluorescence is combined with drug or gene delivery towards theranostic approaches or with complementary technologies for multimodal imaging. We also briefly discuss QD toxicity issues and give a short outlook on future directions of QD-based bioimaging.

show abstract

Cell Nucleus Penetration by Quantum Dots Induced by Nuclear Staining Organic Fluorophore and UV‐Irradiation

Cited by 18 publications

References 20 publications

One‐Pot Green Synthesis of Nitrogen‐Doped Carbon Quantum Dots for Cell Nucleus Labeling and Copper(II) Detection

One‐Pot Green Synthesis of Nitrogen‐Doped Carbon Quantum Dots for Cell Nucleus Labeling and Copper(II) Detection

The Influence on Cell Cycle and Cell Division by Various Cadmium‐Containing Quantum Dots

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Contact Info

Product

Resources

About