Cell-patterned glass spray for direct drug assay using mass spectrometry

Wu, Jing; Wang, Shiqi; Chen, Qiushui; Jiang, Hao; Liang, Shuai; Lin, Jin‐Ming

doi:10.1016/j.aca.2015.08.020

Cited by 14 publications

(9 citation statements)

References 53 publications

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“…[12,32] As a result, the activity of CPA is enhanced in the presence of HepG2 cells. Compared with the former results, [31] it seemed that CPA could easily cross the intestine layer and Caco-2 cells had little effect on its activity. Cell apoptosis caused by Dox exhibited an opposite tendency (Fig.…”

Section: Drug-induced Cell Apoptosis Assaymentioning

confidence: 77%

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Multi‐channel cell co‐culture for drug development based on glass microfluidic chip‐mass spectrometry coupled platform

Jie

Dong

et al. 2016

Rapid Comm Mass Spectrometry

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RATIONALE:Cell-based drug assay plays an essential role in drug development. By coupling a microfluidic chip with mass spectrometry (MS), we developed a multifunctional platform. Cell co-culture, cell apoptosis assay, fluorescence and MS detection of intracellular drug absorption could be simultaneously conducted on this platform. METHODS: Three micro-channels were fabricated through photolithography technology to conduct the cell co-culture. Cell apoptosis after drug treatment was assayed by fluorescent probes (Hoechst 33342). Intracellular Dox absorption was analyzed by confocal fluorescent microscopy. With a high voltage (~4.5 kV) applied onto the microfluidic chip, the ionization spray was successfully generated by dropping isopropanol onto it. By coupling with a Shimadzu LCMS-2010 A mass spectrometer, intracellular CPA absorption was detected on the microfluidic chip. RESULTS: The microfluidic chip-MS coupled platform showed high biocompatibility. Distinction of cell apoptosis between co-cultured and mono-cultured cells was detected. The results of intracellular drug absorption well explained the different cell apoptosis rate. CONCLUSIONS: Cell-based drug assay was facilely and successfully conducted on the microfluidic chip-MS coupled platform. This technology we have devised could promote MS application in the field of drug development. Copyright

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Section: Drug-induced Cell Apoptosis Assaymentioning

confidence: 77%

“…[31] In this work, we describe a novel microfluidic chip-MS coupled platform for direct drug assay with a coverslip as substrate. By combining the advantages of microfluidic technology, the new platform is more advanced than the previous one.…”

mentioning

confidence: 99%

Multi‐channel cell co‐culture for drug development based on glass microfluidic chip‐mass spectrometry coupled platform

Jie

Dong

et al. 2016

Rapid Comm Mass Spectrometry

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“…More simply, Wei et al used a small piece of tissue loaded on the sharp tip of an acupuncture needle, which resulted in a spray under the effect of high voltage and additional solvent [30]. Recently, a spray ionization method based on a single glass slide was also reported [31,32]. By applying a high voltage to the liquid phase sample on the top of a coverslip, the spray was achieved at the corner and the sample was ionized.…”

Section: Introductionmentioning

confidence: 97%

“…By applying a high voltage to the liquid phase sample on the top of a coverslip, the spray was achieved at the corner and the sample was ionized. Jiang et al used the glass spray for real-time monitoring of the ethylene polymerization process [31] and Wu et al used it for the study of a cell-based drug assay [32].…”

Section: Introductionmentioning

confidence: 99%

Interlayer spray ionization mass spectrometry for the simple direct analysis of low amounts of sample

et al. 2016

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Interlayer spray is proposed as a convenient ionization source for direct analysis by mass spectrometry. Two slices of non-absorbent substrate hold the liquid sample to form a sandwich structure. By applying a high voltage to the sample, spray is generated at the tip of the substrate. The sampling procedure can be operated easily in an open condition and the spray is processed in a semi-enclosed condition, which leads to a relatively stable process. An ultralow amount (<2 μL) of the liquid sample can be analyzed without dilution, which ensures that the natural concentration and properties of the target are maintained. Less influence from the substrate is achieved compared with the spray methods based on porous absorbent materials, which results in a sensitivity enhancement of large molecule samples. It is demonstrated that the interlayer spray is applicable for the analysis of various compounds, including therapeutic drugs, peptides, and proteins. Good linearity can be obtained at a concentration as low as 50 ng/mL in the quantitative analysis for imatinib. We also show the ability to identify the chemical residuals on surfaces with high sensitivity by the "wipe-spray" method, which is useful for the fast screening of illicit substances. Interlayer spray working with mass spectrometry provides a promising method for direct analysis in an ambient environment. Graphical Abstract The schematic of the interlayer spray ionization source.

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“…They found anon orientation toward the increasing surface density of laminin. Using a piece of hydrophobic polythyleneterephthalate (PET) film, we separated two kinds of cells on the same coverslip before the following on-line analysis of metabolism [13].…”

Section: Cell and Environment Controlmentioning

confidence: 99%

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

Yi¹,

Lin²

2016

Mol Biol

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Development of microfluidic culture technology combined with tissue engineering catalyzes the progress in study of cell biology. These tools will promote the understanding of physiological and pathological changes. Cancer cells and stem cells are sensitive to their surroundings, thus could be better explored by controllable microfluidic devices. In this review, we describe the ways to control cell microenvironment and explain how the influencing factors influence cellular behaviors, then present microfluidic-chip-based exemplary applications for cancer models and stem cell differentiation.

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Cell-patterned glass spray for direct drug assay using mass spectrometry

Cited by 14 publications

References 53 publications

Multi‐channel cell co‐culture for drug development based on glass microfluidic chip‐mass spectrometry coupled platform

Multi‐channel cell co‐culture for drug development based on glass microfluidic chip‐mass spectrometry coupled platform

Interlayer spray ionization mass spectrometry for the simple direct analysis of low amounts of sample

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

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