Cell polarity regulators, multifunctional organizers of lymphocyte activation and function

High-resolution imaging of protein secretion at the single-cell level using plasmon-enhanced FluoroDOT assay

“…These proteins are secreted in very different manners: TNF-α secretion depends upon cleavage of the membrane-bound precursor by the metalloprotease TNF-α-converting enzyme (TACE), whereas IL-1β secretion depends upon Gasdermin-D pores. The anisotropic nature of TNF-α secretion suggests that some required component is polarized in the cell, perhaps reflecting underlying polarity associated with DC migration ( Mastrogiovanni et al., 2021 ). While the underlying reason behind the anisotropic secretion is a subject of future study, the ability of the FluoroDOT assay to faithfully capture these events further highlights its utility in biomedical research.…”

What makes tics tick? Insights into Tourette syndrome

“…Involved molecules are likely Rho family GTPases, although polarity regulators endeavor numerous protein–protein interactions. Hence, a much wider mode of interplay is suggested [ 22 ].…”

Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulators

“…Cell polarity regulators are scaffold proteins, endowed with a variety of protein-protein interaction motifs, whose effectors are involved in mechanisms controlling cytoskeleton organization, cell shape and symmetry. Several polarity regulators have been involved in T lymphocyte processes, including cell migration and immunological synapse formation (Mastrogiovanni et al, 2021). Among them, Discs large homolog 1 (Dlg1) and Adenomatous polyposis coli (Apc) play key roles in immunological synapse formation and function in CD4 and CD8 T cells, via their control of microtubule network organization at the synapse (Aguera-Gonzalez et al, 2017; Juzans et al, 2020;Lasserre et al, 2010).…”