Cell Proliferation and Migration in the Stomach, Duodenum, and Rectum of Man: Radioautographic Studies

MacDonald, Walter C.; Trier, Jerry S.; Everett, N. B.

doi:10.1016/s0016-5085(64)80102-5

Cited by 253 publications

(70 citation statements)

References 19 publications

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“…The initial progenitor zone observed between 8 and 12 weeks of gestation was situated predominantly in the lower half of the early pit/glands. As the mucosa matures, it subsequently moves upwards, establishing an adult location in the isthmus and neck of glands (Mac Donald et al 1964;Lipkin 1985). These observations of the developing human stomach are closely analogous to earlier findings in the developing rat stomach (Yeomans and Trier 1976).…”

Section: Discussionsupporting

confidence: 85%

“…Over a period of 3 to 4 weeks, the gastric stratified epithelium which contained scattered proliferating cells, is converted into an epithelium containing localized progenitor zones which resemble the patterns in adult gastric mucosa (Mac Donald et al 1964). The initial progenitor zone observed between 8 and 12 weeks of gestation was situated predominantly in the lower half of the early pit/glands.…”

Section: Discussionmentioning

confidence: 99%

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Cell proliferation in developing human stomach

Ménard

Arsenault

1990

Anat Embryol

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Cell proliferation during morphogenesis of human stomach was investigated using radioautography and biochemical determinations of [3H]-thymidine incorporation into DNA. Labeling indices in the epithelium, mesenchyme and muscle layer were established on radioautographs and the heights (mm) of the gastric glands were measured between 10 and 17 weeks of gestation. At 11-12 weeks, the appearance of the first pit/gland was noted, and the labeling index ranged from 9.2 to 10.2%. Labeled cells were present at all levels of the stratified epithelium. Between 14 and 16 weeks, the total epithelial labeling index declined sharply (8.1 to 5.4%) with a concomitant increase of the height of the pit/gland structures (0.055 to 0.080 mm). High proliferative activity was also recorded in the mesenchyme and the muscle layer, the labeling indices decreasing between 10 and 17 weeks. The biochemical quantitation of the [3H]-thymidine uptake into the total gastric DNA clearly supported the continuous decrease of the cell proliferation determined by radioautography. Detailed analysis of the epithelium showed that proliferative cells were more numerous at the base of the gland at the earliest stage (11 weeks) but concentrated in the pit/neck regions by 13-14 weeks. As the pit/gland development proceeded (14 to 17 weeks) labeled cells remained more abundant in the pit/neck regions of the gland (10.9%) and were rarely seen on the surface epithelium (2%). The present investigation provides basic quantitative data regarding cell proliferation in developing human stomach, and indicates that the morphogenesis of the gastric glands is correlated with the high proliferative capacity of the pit/neck cells.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Cell proliferation in developing human stomach

Ménard

Arsenault

1990

Anat Embryol

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“…Mucous neck cells have been identified as providing the generative pool for surface epithelium, parietal cells and chief cells, even entero-endocrine cells, in autoradiographic studies with 3H-thymidin (29,30,31). Mucous neck cells received attention in earlier autoradiographic studies with macromolecular precursor molecules, and their special behavior was recognized.…”

Section: Stomachmentioning

confidence: 99%

“…Cell proliferation, studied with 3H-thymidine autoradiography, is abundant in the lower half of the crypts (29), and it changes with age (65). Similar as in the small intestine, vitamin 0 may be involved in the regulation of cell proliferation.…”

Section: Colonmentioning

confidence: 99%

Vitamin D and the digestive system

Stumpf

2008

Eur. J. Drug Metabol. Pharmacokinet.

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Target tissues of in vivo receptor binding and deposition of 1,25(OH)2 vitamin D3 and its oxygen analog OCT are reviewed in rats, mice, hamsters and zebra finch, identified with high-resolution microscopic autoradiography. Throughout the digestive system numerous sites with nuclear receptor binding of 3H-1,25(OH)2 vitamin D3 and 3H-OCT exist: in the oral region, epithelial cells of the oral cavity, tongue and gingiva, teeth odontoblast and ameloblast precursor pulp and stratum intermedium cells; in the parotid, submandibular and sublingual salivary glands, epithelial cells of striated ducts and granular convoluted tubules, intercalated ducts and acinar cells, as well as myoepithelial cells; in the stomach, neck mucous cells of gastric glands, endocrine cells of the antrum, and muscle cells of the pyloric sphincter; in the small and large intestine, absorptive and crypt epithelial cells; in the pancreas, predominantly islet B-cells. Perisinusoidal stellate (Ito) cells in the liver concentrate and retain variable amounts of radiolabeled compound in regions of their cytoplasm after administration of 3H-I,25(OH)2 vitamin D3 and 3H-25(OH) vitamin D3, probably sites of specific storage, similar to vitamin A. Submucosa in stomach and intestine also retain variable amounts of radiolabel, however unspecific with all compounds studied. In pilot studies with 3H-25(OH)2 vitamin D3 and 3H-24,25(OH)2 vitamin D3, no nuclear concentration was detectable. The reviewed data for vitamin D and its oxygen analogue OCT indicate genomic effects on multiple target tissues of the digestive system that involve cell proliferation and differentiation, endo- and exocrine secretion, digestion and absorption for maintaining optimal functions, with potentials for health prophylaxis and therapies.

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“…1). There has been a general agreement that cell proliferation in the gastric glands occurs in the isthmus region (Plenk 1932;Stevens and Leblond 1953), where cells quickly acquire [ 3 H]thymidine labeling after injection (Messier and Leblond 1960;MacDonald et al 1964;Kataoka et al 1989). This proliferative cell region has been found recently to include various types of epithelial lineage progenitors in mice (Karam and Leblond 1992), rabbits (Karam and Forte 1994), and humans (S.M.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the rabbit gastric epithelial lineage progenitors in short-term culture

Karam

Alexander

Farook

et al. 2001

Cell and Tissue Research

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Little is known about the mechanisms that establish and maintain the proliferation and differentiation programs of the gastric epithelium. This is largely due to the complexity of the gastric epithelial units and the presence of the different epithelial lineage progenitors among heterogeneous populations of various mature cell types. This study is undertaken to establish an in vitro system highly enriched for gastric epithelial lineage progenitors. By using adult male rabbits, a simple method of isolating gastric epithelial cell fractions enriched in lineage progenitors was applied. Cultured cells labeled with bromodeoxyuridine were characterized by using lectin and immunohistochemical studies at light- and electronmicroscopical levels. Analysis of primary cultures derived from the progenitor cell region of the epithelial units revealed that this system can support the proliferation and some of the differentiation programs of the progenitor cells. Cultured cells can be maintained for up to 5 days, while retaining most of the morphological features, molecular markers, and dynamic behavior of gastric epithelial progenitors. Differential cell counts at 1-day culture revealed that, while the undifferentiated progenitors formed about 30% of all attached cells, pre-pit, pit, and preparietal cells represented about 30%, 10%, and 2%, respectively. By 3 days, the increase in the percentage of pit and preparietal cells up to 25% and 9%, respectively, reflected their production in vitro. In conclusion, we have established a culture system enriched for gastric epithelial lineage progenitors that would hopefully allow the identification of factors and mechanisms involved in controlling their proliferative activity and differentiation pathways.

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Cell Proliferation and Migration in the Stomach, Duodenum, and Rectum of Man: Radioautographic Studies

Cited by 253 publications

References 19 publications

Cell proliferation in developing human stomach

Cell proliferation in developing human stomach

Vitamin D and the digestive system

Characterization of the rabbit gastric epithelial lineage progenitors in short-term culture

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