Cell proliferation during the early compartmentalization of the Xenopus laevis inner ear

Quick, Quincy A.; Serrano, Elba E.

doi:10.1387/ijdb.062176qq

Cited by 20 publications

(22 citation statements)

References 48 publications

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“…In order to establish the BrdU as proliferation tracer and subsequent study of cell fate in the olfactory epithelium, we treated tadpoles following the same protocol and conditions proposed by Quick and Serrano for the study of cell proliferation in X. laevis larvae [12].…”

Section: Resultsmentioning

confidence: 99%

“…In our study, we show that BrdU incorporation in proliferative cells of the tadpole OE has a toxic effect in a dose-dependent manner. In previous works in our laboratory and others [12,23] the amphibian larvae immersion in BrdU solution was used to evaluate cell proliferation, however, in these works the analysis was done at short time post-treatment and did not examine cell linage or neuronal differentiation. These last alteration is probably a consequence of whole BrdU effects on the different OE components.…”

Section: Discussionmentioning

confidence: 99%

“…Tadpoles (n=5 per treatment) were treated by immersion in phosphate buffered saline (PBS) with 0, 1, or 10 mM BrdU (SigmaB5002, St. Louis, MO) during 45 min at room temperature (22-24°C) as described by Quick and Serrano (2007). Then, they were maintained in dechlorinated tap water for different times (1, 3, 72 h and 30 days) prior to euthanasia; and processed by different techniques detailed below.…”

Section: Brdu Treatmentmentioning

confidence: 99%

“…They are a suitable option for many reasons. First, BrdU can be added directly into the rearing water tadpoles inhabit [12]. Moreover, these animals have a completely functional olfactory system containing all the representative cell types of a mature system, which in addition possesses embryonic characteristics, so that the mechanisms of cell proliferation and differentiation are particularly active and provide a good opportunity to study regenerative processes [13].…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium

Pozzi¹

2015

MOJAP

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Brdu Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium

Pozzi¹

2015

MOJAP

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“…Cell proliferation, cell rearrangement and neuronal death play an important role in patterning different regions of the brain (Quick and Serrano, 2007;Vecino et al, 2004; LeDouarin, 2001;Fleming et al, 1997;Weil et al, 1997;Ellis et al, 1991). A range of signaling molecules and their roles have been implicated in vertebrate development (Borgave and Ghaskadbi, 2009; SanchezCalderon et al, 2007;Patwardhan et al, 2004;Waschek et al, 1998;Hallbook et al, 1995;Ernfors et al, 1995;Hyuga et al, 1993).…”

Section: Abstract: Chick Embryo Gastrulation Immunocytochemistry mentioning

confidence: 99%

Expression pattern of iodotyrosine dehalogenase 1 (DEHAL1) during chick ontogeny

Mathi¹,

Gaupale²,

Dupuy³

et al. 2010

Int. J. Dev. Biol.

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The iodotyrosine dehalogenase1 (DEHAL1) enzyme is a transmembrane protein that belongs to the nitroreductase family and shows a highly conserved N-terminal domain. DEHAL1 is present in the liver, kidney and thyroid of mammals. DEHAL1 is known to act on diiodotyrosine (DIT) and monoiodotyrosine (MIT), and is involved in iodine recycling in relation to thyroglobulin. Here, we show the distribution of DEHAL1 during gastrulation to neurulation in developing chick. Immunocytochemistry using an anti-serum directed against the N-terminal domain (met 27 -trp 180 fragment) of human DEHAL1 revealed labelled cells in the embryonic ectoderm, embryonic endoderm, neural plate and in the yolk platelets of the chick embryo at gastrulation stage. Distinct DEHAL1 positive cells were located in the presumptive head ectoderm, presumptive neural crest, head mesenchymal cells and in the dorsal, lateral and ventral parts of neural tube during neurulation. Some cells located at the margin of the developing notochord and somites were also DEHAL1-positive. While the functional significance of this observation is not known, it is likely that DEHAL1 might serve as an agent that regulates cell specific deiodination of MIT and DIT before the onset of thyroidal secretion. The presence of DEHAL1 in different components of the chick embryo suggests its involvement in iodine turnover prior to the formation of functional thyroid. KEY WORDS: chick embryo, gastrulation, immunocytochemistry, iodotyrosine dehalogenase 1, neurulationCell proliferation, cell rearrangement and neuronal death play an important role in patterning different regions of the brain (Quick and Serrano, 2007;Vecino et al., 2004; LeDouarin, 2001;Fleming et al., 1997;Weil et al., 1997;Ellis et al., 1991). A range of signaling molecules and their roles have been implicated in vertebrate development (Borgave and Ghaskadbi, 2009; SanchezCalderon et al., 2007;Patwardhan et al., 2004;Waschek et al., 1998;Hallbook et al., 1995;Ernfors et al., 1995;Hyuga et al., 1993). Thyroid hormones are known to play an important role in the early development including neurulation of several vertebrates and previous studies have shown the occurrence of enzymes that are involved in the recycling of Iodine from thyroid hormones (Courtin et al., 2005;Gereben et al., 2004;Geyten et al., 2002;Asteria, 1998;Becker et al., 1997;Rosenkilde and Ussing, 1996).Iodotyrosine dehalogenase 1 (DEHAL1) is a transmembrane protein, localized mainly at the apical pole of thyrocyte in the Int. J. Dev. Biol. 54: 1503-1508 (2010) human thyroid (Gnidehou et al., 2004). DEHAL1 belongs to the nitroreductase family, with a highly conserved N-terminal domain located between Glu 92 and Gly 244 and a putative transmembrane domain between Asn 213 and Gln 229 (Gnidehou et al., 2004). DEHAL1 is quite different from other deiodinases. DEHAL1 acts on diiodotyrosine (L-DIT) and monoiodotyrosine (L-MIT), whereas, deiodinases act on thyroxine (T4) and triiodothyronine (T3) (Solis et al., 2004). The DIT and MIT are released du...

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Localization of Kv2.2 protein in Xenopus laevis embryos and tadpoles

Gravagna¹,

Knoeckel²,

Taylor³

et al. 2008

J of Comparative Neurology

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Voltage-gated potassium (Kv) channels sculpt neuronal excitability and play important developmental roles. Kv channels consist of pore-forming 〈 and auxiliary subunits. For many Kv α-subunits, existing mRNA probes and antibodies have allowed analysis of expression patterns, typically during adult stages. Here, we focus on the Kv2.2 〈-subunit, for which the mRNA shows broad expression in the embryo and adult. A lack of suitable antibodies, however, has hindered detailed analysis of Kv2.2 protein localization, especially during development. We developed an antibody that specifically recognizes Kv2.2 protein in Xenopus laevis, a vertebrate well suited for study of early developmental stages. The Kv2.2 antibody recognized heterologously expressed Kv2.2 but not the closely related Kv2.1 protein. Immunodetection of the protein showed its presence at St 32 in ventrolateral regions of the hindbrain and spinal cord. At later stages, several sensory tissues (retina, otic and olfactory epithelia) also expressed Kv2.2 protein. As development progressed in the central nervous system, Kv2.2 protein distribution expanded in close association with the cytoskeletal marker α-tubulin, consistent with growth of neuronal tracts. We analyzed the subcellular distribution of Kv2.2 protein within single cultured neurons. In addition to a surface membrane presence, Kv2.2 protein also resided intracellularly closely associated with α-tubulin, as in vivo. Further, in contrast to Kv2.1, Kv2.2 protein localized to long axonal-like processes, consistent with its in vivo location in tracts. Despite their primary sequence similarity, the contrasting localizations of Kv2.1 and Kv2.2 support different roles for each during development and neuronal signaling.

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Cell proliferation during the early compartmentalization of the Xenopus laevis inner ear

Cited by 20 publications

References 48 publications

Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium

Cytotoxic Effect of 5-Bromo-2-Deoxyuridine on Olfactory Epithelium

Expression pattern of iodotyrosine dehalogenase 1 (DEHAL1) during chick ontogeny

Localization of Kv2.2 protein in Xenopus laevis embryos and tadpoles

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