Cell proliferation on three-dimensional chitosan–agarose–gelatin cryogel scaffolds for tissue engineering applications

Bhat, Sujata V.; Kumar, Ashok

doi:10.1016/j.jbiosc.2012.07.005

Cited by 91 publications

(46 citation statements)

References 41 publications

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“…Scaffolds were then treated with UV to ensure the proper sterilization. For appropriate cell attachment, scaffolds were saturated in complete DMEM by incubating them in 1 mL media for 1 h (Bhat & Kumar, 2012). Then, 0.5 mL cell suspension was seeded at variable densities depending on the type of experiment, followed by the incubation of the scaffolds at 37 • C in 5 vol% CO 2 at 80-90% humidity.…”

Section: Seeding Of Hdfcs On Scaffoldsmentioning

confidence: 99%

Tissue engineering scaffolds electrospun from cotton cellulose

Cheng

Zhang

et al. 2015

Carbohydrate Polymers

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Section: Seeding Of Hdfcs On Scaffoldsmentioning

confidence: 99%

Tissue engineering scaffolds electrospun from cotton cellulose

Cheng

Zhang

et al. 2015

Carbohydrate Polymers

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“…Cells divide and form clumps simultaneously with the synthesis of ECM which showed that cells get encased in the matrix. For the utility in neural, cardiac and skin tissue engineering, IMR-32, C2C12 and Cos-7 cells should maintain their functionality majorly in terms of the synthesis of extracellular matrix when seeded on scaffolds respectively [9]. SEM images showed that these matrices support the proliferation of cells such as IMR-32, C2C12 and Cos-7 fibroblast cells together with the production of ECM.…”

Section: In Vitro Cell Adhesion By Sem and Fluorescence Staining (Pi)mentioning

confidence: 99%

“…DNA quantification was performed by Hoechst assay to analyze the metabolic activity related to cellular proliferation [9]. From the previous experiments (Fig.…”

Section: Dna Quantification By Hoechst Assaymentioning

confidence: 99%

“…Different conventional scaffolding technologies [3] have been used but cryogelation technique has an advantage over other fabrication methods and provides a suitable engineered 3-D surface known as cryogels similar to the native extracellular matrix. These cryogel matrices posses ideal characteristics like large pores, high porosity, high mechanical strength and scale-up opportunities seem to offer a promising alternative towards the treatment of many diseases [4][5][6][7][8][9][10][11][12]. The aim of this study was to design 3-D cryogels by cryogelation technique which shows its potentiality for different soft tissue engineering applications such as neural, cardiac and skin.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of supermacroporous poly(ethylene glycol)–gelatin cryogel matrix for soft tissue engineering applications

Sharma

Bhat

Nayak

et al. 2015

Materials Science and Engineering: C

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“…It has been combined with CS [67], [68], hyaluronic acid [69], chitosan [70], [71], cellulose [72], agarose [73] and alginate [74] polysaccharides. Tri-copolymers of gelatin with CS and HA [75]- [78], with chitosan and HA [79], and with chitosan and agarose [80] have also been studied. [83].…”

Section: Protein-polysaccharide Hydrogelsmentioning

confidence: 99%

Protein-based injectable hydrogels towards the regeneration of articular cartilage

Poveda-Reyes¹

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A mis seres queridos, con ellos todo es posible "The future belongs to those who believe in the beauty of their dreams"Eleanor Roosevelt Acknowledgements 7 AcknowledgementsAfter reaching this point I would like to thank everybody that in one way or another has helped me through these four years of work.First of all, I gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the DPI2010-20399-C04-03 and MAT2013-46467-C4-1-R projects, my FPI fellowship BES-2011-046144 and the EEBB-I-13-06179 and EEBB-I-14-08725 research stay grants.I would especially like to thank my thesis supervisor, Dr. Gloria Gallego Ferrer, for helping me in everything I needed through these four years, for giving me this opportunity and for all her efforts in my assistance. Also thanks to José Luis Gómez Ribelles for his valuable help and brilliant ideas.Thanks to the staff of the Microscopy Service at the Universitat Politècnica de València, for their guidance and help during the SEM sessions.I would also like to express my gratitude to my supervisors during my stays at other Research Centers. Thanks to Prof. Ulrica Edlund, who during my stay at the Fiber and Polymer Technology Department (KTH) in Stockholm, helped me with everything I could need and to all the people in the center (Laleh, Anas, Soheil, Bekir, Parmida, Robertus, Arturo, etc.) that helped me in the lab and made my stay easier. Thanks to Prof. Manuel Salmerón Sánchez at the Microenvironments for Medicine group (MiMe) (University of Glasgow) for giving me the opportunity to learn the basics of cell culture in his lab, his guidance and valuable help. I would also like to thank Dr. Vladimira Moulisova for teaching me everything I know of cell biology work and being so patient and friendly. And to all the people of the group (Marco, Sara, Eleni, Mark, Alex, Jake, Elie, Frankie, etc.), as they say "People make Glasgow" and they made me feel at home. I cannot forget all the people at the Center for Biomaterials and Tissue Engineering: to Laura Teruel, for being so patient with everyone, so helpful and keeping the lab in a perfect state, and all the lecturers for their help and advice, especially to Ana Vallés and Manuel Monleón, who introduced me to the wonderful field of Tissue Engineering.I would like to especially thank Tatiana C. Gamboa, who guided me in my first years in the lab. To Suhail, Rocío, Marta, Félix, Petra, Leonardo, Rebeca and Esther, I helped them in their first steps in the lab but I learnt a lot from them and they helped me a lot, both professional and personally. Also thanks to Alex Rodrigo who helped me a lot in my first cell cultures even if he had a million things to do.Thanks to all the people who have been in the "Sala de Reuniones" office: Manu, María, Line, Amparo G, Roberto, Guillermo, Álvaro, Laia, Rubén, etc., thanks for the break times, your laughs, your help and being the way you are.Special thanks to my friends, they gave me support through all these years, with them life is better.Finally, I w...

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Cell proliferation on three-dimensional chitosan–agarose–gelatin cryogel scaffolds for tissue engineering applications

Cited by 91 publications

References 41 publications

Tissue engineering scaffolds electrospun from cotton cellulose

Tissue engineering scaffolds electrospun from cotton cellulose

Efficacy of supermacroporous poly(ethylene glycol)–gelatin cryogel matrix for soft tissue engineering applications

Protein-based injectable hydrogels towards the regeneration of articular cartilage

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