Efficacy of supermacroporous poly(ethylene glycol)–gelatin cryogel matrix for soft tissue engineering applications

Sharma, Archana; Bhat, Sujata V.; Nayak, Vijayashree; Kumar, Ashok

doi:10.1016/j.msec.2014.11.031

Cited by 43 publications

(24 citation statements)

References 56 publications

(85 reference statements)

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“…With a reduced crosslinking reaction rate as the temperature falls, the reaction time for cryogel fabrication under sub-zero temperatures must be extended to allow for complete reaction. The fabricated cryogel scaffold has favorable characteristics such as pore interconnectivity, highly porous structure, mechanical stability, and elasticity to be applied as an ideal scaffold for tissue engineering [6,7,8,9]. …”

Section: Introductionmentioning

confidence: 99%

Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering

Chen

Kuo

Wang

et al. 2016

IJMS

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Glucosamine (GlcN) fulfills many of the requirements as an ideal component in scaffolds used in cartilage tissue engineering. The incorporation of GlcN in a gelatin/hyaluronic acid (GH) cryogel scaffold could provide biological cues in maintaining the phenotype of chondrocytes. Nonetheless, substituting gelatin with GlcN may also decrease the crosslinking density and modulate the mechanical properties of the cryogel scaffold, which may be beneficial as physical cues for chondrocytes in the scaffold. Thus, we prepared cryogel scaffolds containing 9% GlcN (GH-GlcN9) and 16% GlcN (GH-GlcN16) by carbodiimide-mediated crosslinking reactions at −16 °C. The crosslinking density and the mechanical properties of the cryogel matrix could be tuned by adjusting the content of GlcN used during cryogel preparation. In general, incorporation of GlcN did not influence scaffold pore size and ultimate compressive strain but increased porosity. The GH-GlcN16 cryogel showed the highest swelling ratio and degradation rate in hyaluronidase and collagenase solutions. On the contrary, the Young’s modulus, storage modulus, ultimate compressive stress, energy dissipation level, and rate of stress relaxation decreased by increasing the GlcN content in the cryogel. The release of GlcN from the scaffolds in the culture medium of chondrocytes could be sustained for 21 days for GH-GlcN16 in contrast to only 7 days for GH-GlcN9. In vitro cell culture experiments using rabbit articular chondrocytes revealed that GlcN incorporation affected cell proliferation, morphology, and maintenance of chondrogenic phenotype. Overall, GH-GlcN16 showed the best performance in maintaining chondrogenic phenotype with reduced cell proliferation rate but enhanced glycosaminoglycans (GAGs) and type II collagen (COL II) secretion. Quantitative real-time polymerase chain reaction also showed time-dependent up-regulation of cartilage-specific marker genes (COL II, aggrecan and Sox9) for GH-GlcN16. Implantation of chondrocytes/GH-GlcN16 constructs into full-thickness articular cartilage defects of rabbits could regenerate neocartilage with positive staining for GAGs and COL II. The GH-GlcN16 cryogel will be suitable as a scaffold for the treatment of articular cartilage defects.

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Section: Introductionmentioning

confidence: 99%

Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering

Chen

Kuo

Wang

et al. 2016

IJMS

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“…Repeated freeze‐thaw method was adopted for synthesis of cryogels of starch and PVA . In a typical experiment, separate aqueous solutions of PVA (11.7 wt%) and starch (21 wt%) were homogenously mixed together and 0.55 wt% of silver hydroxyapatite was added to it.…”

Section: Methodsmentioning

confidence: 99%

Silver hydroxyapatite reinforced poly(vinyl alcohol)—starch cryogel nanocomposites and study of biodegradation, compressive strength and antibacterial activity

Bagri

Saini

Bajpai

et al. 2018

Polymer Engineering & Sci

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In the present work polyvinyl alcohol‐starch/silver hydroxyapatite (PVA‐starch/AgHap) cryogel nanocomposites were prepared by successive freezing‐thawing of a blend of PVA and starch solutions to fabricate a cryogel followed by its reinforcement with silver hydroxyapatite (AgHap). The prepared macroporous cryogel nanocomposites were characterized by Infra‐red spectroscopy (FTIR), environmental scanning electron microscopy (ESEM), and particle size and charge analysis. The amylase induced enzymatic degradation of nanocomposites was studied gravimetrically in phosphate buffer saline (PBS) and effect of various parameters like chemical composition of the nanocomposite, number of freeze‐thaw cycles, and enzyme activity were assessed on the extent of degradation of the nanocomposite. The influence of chemical composition and experimental conditions like the number of freeze thaw cycles was studied on the elastic modulii of the cryogels. The in vitro cytotoxicity and antibacterial activity of nanocomposites was also evaluated against L‐529 fibroblast cells and gram positive and gram negative bacteria, respectively. POLYM. ENG. SCI., 59:254–263, 2019. © 2018 Society of Plastics Engineers

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“…Synthetic biomaterials have the advantage of being tailored and specifically designed for adjustment of mechanical/chemical properties as well as degradation ( Place et al, 2009 ). Synthetic materials such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA), and poly(lactic-co-glycolic acid) (PLGA) have been extensively investigated and used for adipose tissue regeneration ( Patrick et al, 1999 , 2002 ; Patrick, 2001 ; Place et al, 2009 ; Itoi et al, 2010 ; Dhandayuthapani et al, 2011 ; Sharma et al, 2015 ; Elamparithi et al, 2016 ). These polymers degrade via hydrolysis and their degradability can be controlled by altering the molecular weight, crystallinity, and ratio of lactic to glycolic acid subunits ( Place et al, 2009 ).…”

Section: Off-the-shelf Biotechnologies and Scaffoldsmentioning

confidence: 99%

Current Therapeutic Strategies for Adipose Tissue Defects/Repair Using Engineered Biomaterials and Biomolecule Formulations

et al. 2018

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Tissue engineered scaffolds for adipose restoration/repair has significantly evolved in recent years. Patients requiring soft tissue reconstruction, caused by defects or pathology, require biomaterials that will restore void volume with new functional tissue. The gold standard of autologous fat grafting (AFG) is not a reliable option. This review focuses on the latest therapeutic strategies for the treatment of adipose tissue defects using biomolecule formulations and delivery, and specifically engineered biomaterials. Additionally, the clinical need for reliable off-the-shelf therapies, animal models, and challenges facing current technologies are discussed.

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Efficacy of supermacroporous poly(ethylene glycol)–gelatin cryogel matrix for soft tissue engineering applications

Cited by 43 publications

References 56 publications

Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering

Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering

Silver hydroxyapatite reinforced poly(vinyl alcohol)—starch cryogel nanocomposites and study of biodegradation, compressive strength and antibacterial activity

Current Therapeutic Strategies for Adipose Tissue Defects/Repair Using Engineered Biomaterials and Biomolecule Formulations

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