Cell sorting by deterministic cell rolling

Choi, Sungyoung; Karp, Jeffrey M.; Karnik, Rohit

doi:10.1039/c2lc21225k

Cited by 77 publications

(81 citation statements)

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“…For cells that do not contain granules, the cell surface in principle can be tagged sparsely by functionalized gold nanoparticles, which strongly scatter light and are visible under darkfield microscopy. Quantitative understanding of the adhesion behavior of rolling cells is important not only for fundamental cell biology, but also for practical applications including cancer diagnostics, screening and treatment [9][10][11] . The approaches presented here represent a first step toward a comprehensive label-free cell surface adhesion mapping and characterization technique.…”

Section: Discussionmentioning

confidence: 99%

“…Rolling adhesion behavior is also exhibited by circulating tumor cells (CTCs) which is believed to enhance cancer metastasis [5][6][7][8] . Therefore, quantitative understanding of rolling adhesion is necessary to enable practical applications such as cancer screening and treatment [9][10][11] . At the molecular level, this adhesion is mediated by catch-bond-like interactions 12,13 between P- 14 and E-selectins 15 expressed on endothelial cells lining blood vessels and P-selectin glycoprotein ligand-1 (PSGL-1) found at microvilli tips of leukocytes 16 .…”

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confidence: 99%

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Mapping Cell Surface Adhesion by Rotation Tracking and Adhesion Footprinting

Chemla

2017

Biophysical Journal

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Rolling adhesion, in which cells passively roll along surfaces under shear flow, is a critical process involved in inflammatory responses and cancer metastasis. Surface adhesion properties regulated by adhesion receptors and membrane tethers are critical in understanding cell rolling behavior. Locally, adhesion molecules are distributed at the tips of membrane tethers. However, how functional adhesion properties are globally distributed on the individual cell's surface is unknown. Here, we developed a label-free technique to determine the spatial distribution of adhesive properties on rolling cell surfaces. Using dark-field imaging and particle tracking, we extract the rotational motion of individual rolling cells. The rotational information allows us to construct an adhesion map along the contact circumference of a single cell. To complement this approach, we also developed a fluorescent adhesion footprint assay to record the molecular adhesion events from cell rolling. Applying the combination of the two methods on human promyelocytic leukemia cells, our results surprisingly reveal that adhesion is non-uniformly distributed in patches on the cell surfaces. Our label-free adhesion mapping methods are applicable to the variety of cell types that undergo rolling adhesion and provide a quantitative picture of cell surface adhesion at the functional and molecular level.Rolling adhesion is a common process by which cells attach themselves to surfaces under shear flow, such as in the circulatory system. Leukocytes in the blood utilize this mechanism to locate inflammation sites throughout the body. During an inflammation response, endothelial cells lining the blood vessels surrounding an infection site express adhesion proteins called selectins that are specific to leukocyte surface receptors. As the first step of the leukocyte adhesion cascade, leukocytes captured via selectin-specific interactions passively roll on the blood vessel wall under blood flow toward the inflammation site in a process known as rolling adhesion [1][2][3] . Malfunction of any adhesion molecules involved in this process leads to severe immune disorders such as the leukocyte adhesion deficiencies (LAD) 4 . Rolling adhesion behavior is also exhibited by circulating tumor cells (CTCs) which is believed to enhance cancer metastasis [5][6][7][8] . Therefore, quantitative understanding of rolling adhesion is necessary to enable practical applications such as cancer screening and treatment [9][10][11] . At the molecular level, this adhesion is mediated by catch-bond-like interactions 12,13 between P-14 and E-selectins 15 expressed on endothelial cells lining blood vessels and P-selectin glycoprotein ligand-1 (PSGL-1) found at microvilli tips of leukocytes 16 . Despite our understanding of the individual components, how the molecular details of adhesion bonds scale to cell-surface adhesion and rolling behavior remains poorly understood 2,17,18 . Here, we developed a label-free method that maps the functional adhesion sites and strengths on a cell ...

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Mapping Cell Surface Adhesion by Rotation Tracking and Adhesion Footprinting

Chemla

2017

Biophysical Journal

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“…Karnik and his colleagues introduced a cell rolling microfl uidics device in which the microfl uidic channels were coated with P-selectin to guide the target cells into an isolating chamber ( Fig. 11.6a) (Choi et al ., 2012;Lee et al ., 2011). In addition to the surface protein targeting strategy, Huang and colleagues introduced microfl uidic size-based particle separation (Huang et al ., 2004).…”

Section: Miniaturized Conventional Technologies For Cell Analysismentioning

confidence: 99%

“…(a) Scalable parallel sorting device (Choi et al ., 2012). Deterministic cell rolling based cell sorting (cell surface marker targeting).…”

Section: Miniaturized Devices For Cell Culturementioning

confidence: 99%

Microfluidic devices for stem cell analysis

Kang¹,

Lu²,

Zhang

et al. 2013

Microfluidic Devices for Biomedical Applications

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“…It is interesting to note that up to now we assumed that the particles are solely exposed to magnetic and hydrodynamic forces. Nevertheless, other surface interactions of different origin, such as electrostatic, chemical or friction forces, may introduce an additional torque 37 , which would even be more beneficial for enhancing this reorientation phenomenon.…”

mentioning

confidence: 99%

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

et al. 2014

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ABSTRACT. We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ags) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive stochastic reorientations in an engineered magnetic energy landscape. The latter consists of a pattern of substrate-bound small magnetic particles that are functionalized with antibodies (Abs). Subsequent counting of the captured antigen-carrying particles provides the detection signal. The magnetic particle-scanning principle is investigated in a custom-built magneto-microfluidic chip and theoretically described by a random walk-based model, in which the trajectory of the contact point between an antigen-carrying particle and the small magnetic particle pattern is described by

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Cell sorting by deterministic cell rolling

Abstract: This communication presents the concept of “deterministic cell rolling”, which leverages transient cell-surface molecular interactions that mediate cell rolling to sort cells with high purity and efficiency in a single step.

Cited by 77 publications

References 32 publications

Mapping Cell Surface Adhesion by Rotation Tracking and Adhesion Footprinting

Mapping Cell Surface Adhesion by Rotation Tracking and Adhesion Footprinting

Microfluidic devices for stem cell analysis

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

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