Cell sorting using a universally applicable affinity chromatography matrix: Solid-phase anti-fluorescein isothiocyanate antibody

Baran, Madelyn M.; Allen, Diane M.; Russell, Susan; Scheetz, Maurice E.; Monthony, James F.

doi:10.1016/0022-1759(82)90179-x

Cited by 17 publications

(5 citation statements)

References 22 publications

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“…In general, the contact area will depend on a balance of forces: 0 = Fd,POsi1itona + F,n05pcjfic (S, Aj) + Fdcformation (S, Aj), (12) in which AC is the contact area and S is the separation distance. We intend to calculate how adhesiveness varies with changes in the contact area.…”

Section: Nb =0mentioning

confidence: 99%

“…Interest in cell adhesion in vivo has led to many in vitro experiments, such as the parallel plate flow chamber assay (5-7), designed to understand the physical and chemical processes that control adhesion. Also, the knowledge that subpopulations of cells have unique surface compositions has led to the development of cell separation techniques, like cell affinity chromatography (8)(9)(10)(11)(12)(13), which rely on the differential adhesiveness of these subpopulations to prepared substrates. Thus, a fundamental, quantitative understanding of the adhesion of cells to surfaces can lead to understanding of in vivo phenomena, interpretation of in vitro assays, and analysis and design of biotechnological processes, which involve cell adhesion.…”

Section: Introductionmentioning

confidence: 99%

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A dynamical model for receptor-mediated cell adhesion to surfaces

Hammer

Lauffenburger

1987

Biophysical Journal

253

178

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We present a dynamical model for receptor-mediated adhesion of cells in a shear field of viscous fluid to surfaces coated with ligand molecules complementary to receptors in the cell membrane. We refer to this model as the "point attachment model" because it considers the contact area between the cell and the surface to be a small, homogeneous region that mediates the initial attachment of the cell to the surface. Using a phase plane analysis of a system of nonlinear ordinary differential equations which govern the changes in free receptor density and bond density within the contact area with time, we can predict the conditions for which adhesion between the cell and the surface will take place. Whether adhesion occurs depends on values of dimensionless quantities that characterize the interaction of the cell and its receptors with the surface and its ligand, such as the bond formation rate, the receptor-ligand affinity, the fluid mechanical force, the receptor mobility, and the contact area. A key result is that there are two regimes in which different chemical and physical forces dominate: a rate-controlled high affinity regime and an affinity-controlled low-affinity regime. Many experimental observations can be explained by understanding which of these regimes is appropriate. We also provide simple approximate analytical solutions, relating adhesiveness to cell and surface properties as well as fluid forces, which allow convenient testing of model predictions by experiment.

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Section: Nb =0mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A dynamical model for receptor-mediated cell adhesion to surfaces

Hammer

Lauffenburger

1987

Biophysical Journal

253

178

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“…This toxic effect in vivo was observed in the present study with the PGP-1-stained stem cells. Only procedures that exploit antibodies that label all cells except the stem cells (negative selection) can be predicted to be useful for rapid large-scale purification (Basch et al, 1983) employing batch procedures such as panning (Hoang et al, 19831, magnetic beads (Rembaum et al, 1982), columns (Baran et al, 1982), and rosetting techniques (Wormmeester et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

A fractionation procedure of mouse bone marrow cells yielding exclusively pluripotent stem cells and committed progenitors

Bauman

Wagemaker

Visser

1986

Journal Cellular Physiology

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The cell surface phenotype of pluripotent hemopoietic stem cells (CFU-S) and committed progenitors (CFU-C1, CFU-C2, BFU-E) of mouse bone marrow was analyzed with respect to their binding of wheat germ agglutinin (WGA) and two monoclonal antibodies, anti-GM-1.2 and anti-PGP-1. Stained cells were fractionated on the basis of differences in fluorescence and light scatter intensity using a light-activated cell sorter. The 6% of the cells that bound most WGA and that also had a relatively high forward light scatter (FLS) and low perpendicular light scatter (PLS) contained nearly all stem cells (CFU-S) and progenitors. Anti-GM-1.2 stained only mature myeloid cells, not CFU-S or the in vitro colony-forming cells. Anti-PGP-1 stained all bone marrow cells in varying intensities: lymphoid cells were dull, CFU-S were intermediate, CFU-C2 were brighter, and mature myeloid cells very bright. Enrichment of progenitor cells was performed by a two-step sorting procedure. First, the 6% most WGA-binding cells with high FLS and low PLS were sorted out. A 10-15-fold enrichment of progenitors and CFU-S was obtained. Next, these cells were restained with anti-GM-1.2 or anti-PGP-1 and again fractionated on the FACS. The GM-1.2-negative cells were then another four- to sevenfold more enriched for stem cells and progenitors. Of the cells in this fraction, 95% could be assigned to a colony-forming unit. With anti-PGP-1, CFU-C2 could be partly separated from more early cells such as CFU-S and BFU-E.

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“…One of the earliest approaches for fractionating cell populations by immobilization was through affinity chromatography, which sorts by the high affinity interactions between cell-surface receptors and immobilized ligands on the chromatography matrix. 211,212 This technique is capable of yielding results that do not depend on the size, density, or charge of the cells, and can be performed in various types of matrices. 213 Many of the more recent cell immobilization devices use antibodies for cell-specific isolation, often from whole blood.…”

Section: Recently This System Was Used Bymentioning

confidence: 99%

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

2015

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Accurate and high throughput cell sorting is a critical enabling technology in molecular and cellular biology, biotechnology, and medicine. While conventional methods can provide high efficiency sorting in short timescales, advances in microfluidics have enabled the realization of miniaturized devices offering similar capabilities that exploit a variety of physical principles. We classify these technologies as either active or passive. Active systems generally use external fields (e.g., acoustic, electric, magnetic, and optical) to impose forces to displace cells for sorting, whereas passive systems use inertial forces, filters, and adhesion mechanisms to purify cell populations. Cell sorting on microchips provides numerous advantages over conventional methods by reducing the size of necessary equipment, eliminating potentially biohazardous aerosols, and simplifying the complex protocols commonly associated with cell sorting. Additionally, microchip devices are well suited for parallelization, enabling complete lab-on-a-chip devices for cellular isolation, analysis, and experimental processing. In this review, we examine the breadth of microfluidic cell sorting technologies, while focusing on those that offer the greatest potential for translation into clinical and industrial practice and that offer multiple, useful functions. We organize these sorting technologies by the type of cell preparation required (i.e., fluorescent label-based sorting, bead-based sorting, and label-free sorting) as well as by the physical principles underlying each sorting mechanism.

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Cell sorting using a universally applicable affinity chromatography matrix: Solid-phase anti-fluorescein isothiocyanate antibody

Cited by 17 publications

References 22 publications

A dynamical model for receptor-mediated cell adhesion to surfaces

A dynamical model for receptor-mediated cell adhesion to surfaces

A fractionation procedure of mouse bone marrow cells yielding exclusively pluripotent stem cells and committed progenitors

Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation

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