Cell source-dependent in vivo immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs

Lee, Won–Jae; Hah, Young‐Sool; Ock, Sun‐A; Lee, Jae‐Hoon; Jeon, Ryoung-Hoon; Park, Jisung; S, Lee; Rho, Na-Young; Rho, Gyu‐Jin; Lee, Sung‐Lim

doi:10.1016/j.yexcr.2015.03.015

Cited by 41 publications

(70 citation statements)

References 56 publications

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“…Much of the initial MSC research was focused on bone marrow MSC (BM-MSC) populations; however, it is now well established that MSCs are present in most tissues and body fluids. Despite similarities in isolation protocols, MSCs derived from different tissues demonstrate significant variation in their ability to adopt specific phenotypes, synthesize functional extracellular matrices [3][4][5] and modulate immunological and inflammatory pathways during tissue repair [6,7].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

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Background: Progenitor cells exist in most tissues and body fluids. Synovial fluid chondroprogenitor cells have been described in several species; however, the specific phenotypic characteristics of these cells have not been defined. This study addressed the impacts of joint location and donor age variation on synovial fluid chondroprogenitor numbers, and determined whether synovial fluid chondroprogenitors express hypertrophic characteristics or a non-endochondral phenotype in chondrogenic culture. Methods: Synovial fluid was aspirated from the joints of healthy adult horses, and cells in the aspirates were expanded through two monolayer passages. The number of colony-forming units (CFU) in each aspirate was assessed after 14 days. Population doublings (PD) and PD times were calculated during the subsequent two passages. Passage 3 cells were used to determine osteogenic and adipogenic capacities, or were transferred to pellet cultures in chondrogenic medium containing TGF-β1 or BMP-2. Bone marrowderived MSCs and primary articular chondrocytes were cultured under similar conditions to provide reference values for chondrogenesis. Chondrogenesis was assessed by measuring collagen type II protein and sulfated glycosaminoglycan secretion, expression of chondrogenic mRNAs, and induction of ALP activity. Results: CFU counts varied considerably, but the majority of aspirates contained <50 CFU/ml. PDs were consistently between 2.0 and 3.0 during both passages. PD times varied from 0.2-0.4 days. Proliferation was not significantly influenced by anatomical location or donor age. Equine synovial fluid progenitors expressed osteogenic, adipogenic and chondrogenic phenotypes under appropriate conditions. Under chondrogenic conditions, synovial fluid cells increased collagen and aggrecan mRNA expression to levels comparable to bone marrow MSCs, although collagen type II protein secretion was less than half that of articular chondrocytes. In contrast to bone marrow MSCs, synovial fluid chondroprogenitors did not express collagen type X or increase ALP activity in response to TGF-β1 or BMP-2. Consistent with these observations, Runx2 and Mef2C expression in synovial fluid chondroprogenitors was 20-40 fold lower than in bone marrow MSCs. Conclusions: Synovial fluid chondroprogenitors are capable of robust non-hypertrophic chondrogenesis, whether stimulated by TGF-β1 or by BMP-2. These results indicate that synovial fluid cells are phenotypically suitable for articular cartilage repair/regeneration, although strategies to accelerate cell proliferation and synthesize a functional cartilage matrix in vivo will be required for clinically feasible cellbased therapies.

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Section: Introductionmentioning

confidence: 99%

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Chen¹,

Bianchessi²,

Pondenis³

et al. 2016

Stem Cell Biol Res

View full text Add to dashboard Cite

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“…Their study also demonstrated that MSCs could inhibit activation of T cells and induce Tregs expansion in RA [22]. Another study on synovial fluid (SF)-MSCs showed that the immunosuppressive properties of these MSCs in CIA mice were associated with increased levels of the anti-inflammatory cytokine IL-10, and decreased levels of the proinflammatory cytokine IL-1β [23].…”

Section: Introductionmentioning

confidence: 96%

Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation

Baharlou

Ahmadi-Vasmehjani

Faraji

et al. 2017

International Immunopharmacology

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“…Mesenchymal stem cells (MSCs), derived from mesodermal cell lineages with self‐renewing capacities and multipotential differentiation, have profound therapeutic potential in many diseases, such as tissue engineering and regeneration, autoimmune disease, and myocardial infarction (Jungebluth et al, ; Lee et al, ; Wang et al, ). Moreover, MSCs showed low immunogenicity and transplantation of allogeneic MSCs would not cause immune rejections.…”

Section: Introductionmentioning

confidence: 99%

Isolation and biological characterization of a novel type of pulmonary mesenchymal stem cells derived from Wuzhishan miniature pig embryo

Guo²,

Liu

et al. 2016

Cell Biology International

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Pulmonary mesenchymal stem cells (PMSCs) have great potential in lung tissue engineering and represent attractive candidates for disease treatment in human and veterinary research. However, a reliable method for isolation and localization of porcine PMSCs in situ is still uncertain. In this study, we successfully isolated PMSCs from Wuzhishan miniature pig embryos in vitro and also attempted to unravel its fundamental differentiation potential and biological characteristics. The isolated PMSCs, which could be cultured and passaged for at least 15 passages, exhibited a typical fibroblast-like morphology and high proliferative potential. Moreover, the PMSCs could express pluripotent marker genes (Oct4 and Nanog) and MSCs-related surface antigens (β-integrin, CD44, CD71, CD73, CD90, and CD105), while the expressions of CD34 and CD45 were negative. Cell cycle examination showed that the rate of G0/G1 was about 72.1-90.2%. Additionally, the PMSCs not only could be induced to transdifferentiate into mesoblastic cells such as osteoblasts, chondrocytes, and adipocytes in vitro, but also the neural ectoderm and endoderm. Together, these data demonstrate the multiple differentiations potential of PMSCs in vitro, which confers potential use in serving as desirable cell types for lung injury regeneration.

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Cell source-dependent in vivo immunosuppressive properties of mesenchymal stem cells derived from the bone marrow and synovial fluid of minipigs

Cited by 41 publications

References 56 publications

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Phenotypic characterization of equine synovial fluid-derived chondroprogenitor cells

Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation

Isolation and biological characterization of a novel type of pulmonary mesenchymal stem cells derived from Wuzhishan miniature pig embryo

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