Cell-specific adaptation of two flaviviruses following serial passage in mosquito cell culture

Ciota, Alexander T.; Lovelace, Amy O.; Ngo, Kiet; Le, An N.; Maffei, Joseph G.; Franke, Mary A.; Payne, Anne F.; Jones, Susan; Kauffman, Elizabeth B.; Kramer, Laura D.

doi:10.1016/j.virol.2006.08.005

Cited by 78 publications

(113 citation statements)

References 40 publications

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“…Using virus production as a surrogate, most of the time there is evidence of adaptation [Chen et al, 2003;Ciota et al, 2007bCiota et al, , 2008Ciota et al, , 2009Coffey et al, 2008;Cooper and Scott, 2001;Llewellyn et al, 2002;Moutailler et al, 2011;Romanova et al, 2007;Vasilakis et al, 2009], but occasionally there are no measurable changes [Ciota et al, 2007c]. It is possible that continuous passages result in no fitness changes if the virus is already highly adapted to the environment.…”

Section: Cost Of Specialization and Generalizationmentioning

confidence: 99%

Specific and Nonspecific Host Adaptation during Arboviral Experimental Evolution

Novella

Presloid²,

Smith³

et al. 2011

Microb Physiol

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During the past decade or so, there has been a substantial body of work to dissect arboviral evolution and to develop models of adaptation during host switching. Regardless of what species serve as host or vectors, and of the geographic distribution and the mechanisms of replication, arboviruses tend to have slow evolutionary rates in nature. The hypothesis that this is the result of replication in the disparate environments provided by host and vector did not receive solid experimental support in any of the many viral species tested. Instead, it seems that from the virus’s point of view, either the two environments are sufficiently similar or one of the environments so dominates viral evolution that there is tolerance for suboptimal adaptation to the other environment. Replication in alternating environments has an unexpected cost in that there is decreased genetic variance that translates into a compromised adaptability for bypassed environments. Arboviruses under strong and continuous positive selection may have unusual patterns of genomic changes, with few or no mutations accumulated in the consensus sequence or with dN/dS values typically consistent with random drift in DNA-based organisms.

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Section: Cost Of Specialization and Generalizationmentioning

confidence: 99%

Specific and Nonspecific Host Adaptation during Arboviral Experimental Evolution

Novella

Presloid²,

Smith³

et al. 2011

Microb Physiol

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“…Many amino acid motifs in the E, NS3 and NS5 proteins are shared between ROCV and other mosquito-borne, tick-borne and no-vector flaviviruses. In the E protein, the tripeptide in the domain III region (amino acid residues 388-390), which corresponds to the RGD motif in JEV, Alfuy virus (ALFV), USUV and MVEV (Bakonyi et al, 2004;Hurrelbrink et al, 1999;May et al, 2006;Sumiyoshi et al, 1987), RGE in WNV and KUNV (Castle et al, 1985;Coia et al, 1988) and RGP in SLEV (Ciota et al, 2007), is TGP in ROCV. The TGP peptide is also found in Kedougou virus (KEDV) and is very similar to those of Kokobera virus (KOKV) and BAGV (TGE) (Kuno & Chang, 2007).…”

Section: Motifsmentioning

confidence: 99%

Complete genome characterization of Rocio virus (Flavivirus: Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in São Paulo state

Medeiros

Nunes

Vasconcelos

et al. 2007

Journal of General Virology

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The flaviviruses of major medical importance in South American countries are yellow fever, dengue, Saint Louis encephalitis, West Nile and Rocio viruses. Rocio virus (ROCV) has been responsible for epidemics of severe encephalitis in Brazil with a case-fatality rate of 10 % and development of sequelae in 20 % of the survivors. We have sequenced and characterized the entire genome of ROCV for the first time, by determining the general traits of the open reading frame and the characteristics of viral genes including the potential cleavage sites, conserved or unique motifs, cysteine residues and potential glycosylation sites. The conserved sequences in the 39-non-coding region were identified, and the predicted secondary structures during cyclization between 59-and 39-non-coding regions were studied. Multiple protein and phylogenetic analyses based on antigenically important and phylogenetically informative genes confirmed a close relationship between ROCV and Ilheus virus (ILHV), together constituting a unique and distinct phylogenetic subgroup as well as the genetic relationship of ROCV with several members of the Japanese encephalitis group. Although ROCV is phylogenetically related to ILHV, our study shows that it is still a virus distinct from the latter virus. This is the first flavivirus uniquely indigenous to Brazil that has been sequenced completely and the genome characterized. The data should be useful for further studies at the molecular level, including construction of infectious clone, identification of gene function, improved disease surveillance based on molecular diagnostic tools and vaccine development. INTRODUCTIONIn South America, until the mid-1970s, the two neurotropic flaviviruses had been Saint Louis encephalitis virus (SLEV) and Ilheus virus (ILHV). However, in contrast to North America, an epidemic of encephalitis in humans by SLEV in South America was not recorded for many years until the first outbreak in Argentina in 2005(Diaz et al., 2006, despite accumulated records of virus isolation from mosquitoes and vertebrate hosts throughout the continent (Spence, 1980). As for the other virus, ILHV, human infection was sporadic and development of neurotropic manifestations has been even more infrequent. Thus, the sudden emergence of an outbreak of encephalitis caused by a flavivirus in 1974-75 in Brazil was unexpected.Rocio virus (ROCV) belongs to the genus Flavivirus in the family Flaviviridae. Its prototype (strain SPH 34675) was isolated in Ribeira Valley in the south-eastern São Paulo state of Brazil in 1975 from the cerebellum of a fatal human case during an epidemic of encephalitis that could not be readily diagnosed with the reagents then available for known encephalitic viruses. The epidemic, which began in 1974, spread to more than 20 municipalities over the following 2 years causing approximately 1000 diagnosed cases.Members of the genus Flavivirus (hereafter called flaviviruses) are positive-sense, single-stranded RNA viruses that infect humans and other vertebrates. They are mainly...

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“…During the weekly scheduled bleeding of the fl ocks, 1,338 cloacal swabs were collected in viral culturettes (Becton Dickinson, Franklin Lakes, NJ, USA); 529 swabs were retrospectively processed for molecular detection assays and virus isolation in Vero cells, as previously described (12). Viral RNA was extracted from cloacal swabs and fi rst-passage cell cultures and amplifi ed with real-time reverse transcription-PCR (RT-PCR) TaqMan assays for WNV and SLEV, as previously described (13).…”

Section: The Studymentioning

confidence: 99%

Isolation of Genotype V St. Louis Encephalitis Virus in Florida

Ottendorfer¹,

Ambrose²,

White³

et al. 2009

Emerg. Infect. Dis.

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Cell-specific adaptation of two flaviviruses following serial passage in mosquito cell culture

Cited by 78 publications

References 40 publications

Specific and Nonspecific Host Adaptation during Arboviral Experimental Evolution

Specific and Nonspecific Host Adaptation during Arboviral Experimental Evolution

Complete genome characterization of Rocio virus (Flavivirus: Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in São Paulo state

Isolation of Genotype V St. Louis Encephalitis Virus in Florida

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