Cell specific differences between human adipose-derived and mesenchymal–stromal cells despite similar differentiation potentials

Abstract

Stromal cells from bone marrow and adipose tissue are attractive sources of adult progenitors for cell-based therapy. However, whether those cell populations represent intrinsically different cell types is still largely under debate. The aim of this study was to systematically and quantitatively compare adipose-derived stromal cells (ADSC) and bone marrow-derived multipotent mesenchymal-stromal cells (BM-MSC). The quantitative comparison was realized using Taqman Low Density Array, 2D electrophoresis and differentiation functional assays in vitro. Furthermore, cells engineered to express TGFbeta1 were injected into the intra-articular space of mouse knee joints in order to determine whether they were able to form new differentiated tissues in vivo. Our data revealed cell specific differences at transcriptional and proteomic levels between both cell types according to their tissue origin as well as functional differences in their differentiation processes towards adipogenic, osteogenic and chondrogenic programs. Nevertheless, in vitro as well as in vivo ADSC displayed the same ability than MSC to differentiate towards chondrocytes/osteoblasts, comforting the status of both cell sources as promising regenerative cells. In summary, our observations indicate that ADSC and MSC are fundamentally different cell types and differently committed cells.

Native Adipose Stromal Cells Egress from Adipose Tissue In Vivo: Evidence During Lymph Node Activation

“…AT is composed of adipocytes and various cell types among which are a population of multipotent progenitors, termed adipose stromal cells (ASCs) [4]. ASCs display similar but not identical features with their bone marrow (BM) counterparts, mesenchymal stromal cells (MSCs) [5][6][7][8][9]. Like MSCs, ASCs are endowed with multilineage mesodermal differentiation potentials and exhibit extensive paracrine and immune-modulatory properties [10][11][12][13][14][15].…”

Transplantation of Adipose Tissue-Derived Stem Cells Overexpressing Heme Oxygenase-1 Improves Functions and Remodeling of Infarcted Myocardium in Rabbits

“…Recent experimental studies have shown that adipose tissue-derived stem cells (ADSCs), a source of mesenchymal stem cells (MSCs), are self-renewing and capable of regenerating infracted myocardium by inducing myogenesis and angiogenesis (Hwangbo et al 2010;Lin et al 2010;Yu et al 2010). ADSCs and MSCs exhibit a highly similar gene expression profile (Noel et al 2008), and the stem cells isolated from adipose tissues share the same morphology, phenotype and in vitro differentiation ability with bone marrow. Moreover, cultured populations of ADSCs can be expanded rapidly, and long-term cultured cells retain their mesenchymal pluripotency (Ranera et al 2011).…”

Strategies for Bioengineered Scaffolds that Support Adipose Stem Cells in Regenerative Therapies

“…Investigations into the gene expression profiles of BM-MSCs and ASCs found that 13.2% of 384 genes examined were differentially expressed between the two populations. Although no identifying markers were specific to each population, genes more highly expressed by ASCs were mainly involved in cellular communication (FGF9, IL1R2, CCL3 and KDR) while those with higher expression by BM-MSCs were involved in WNT signaling and differentiation pathways (WNT11, WNT7B and SOX6 ) [25].…”