Cell specific expression of three genes involved in plasma membrane sucrose transport in developingVicia faba seed

Harrington, Gregory N.; Franceschi, Vincent R.; Offler, Christina E.; Patrick, John W.; Tegeder, Mechthild; Frommer, W. B.; Harper, Jeffrey F.; Hitz, William D.

doi:10.1007/bf01288025

Cited by 82 publications

(84 citation statements)

References 28 publications

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“…For example, strong proton pump activities have been detected in plasma membranes of ground parenchyma cells proximal to vascular bundles and thin-wall parenchyma transfer cells of Phaseolus and Vicia seed coats, respectively . That thin-walled parenchyma transfer cells of Vicia seed coats are enriched in transporter proteins was verified by histochemical detection of high densities of H + -ATPases, sucrose binding proteins (Harrington et al 1997a) and an amino acid permease, VfAAP1 (Miranda et al 2001). Similar studies have established that sucrose transporter proteins and active H + -ATPases are enriched in nucellar tissues of developing grains of barley (Weschke et al 2000), wheat (Bagnall et al 2000) and rice .…”

Section: Cellular Site(s) Of Exchange To the Seed Apoplasmmentioning

confidence: 68%

“…1). High densities of H + -ATPases, localised to plasma membranes of seed coat cells putatively responsible for nutrient release Harrington et al 1997a), could function to generate the PMF driving sucrose/H + antiport (Fig. 1).…”

Section: Sucrose and Amino Acidsmentioning

confidence: 99%

“…These events are accompanied by elevated expression levels of sucrose transporter genes in developing seeds of dicots (Weber et al 1997a;Harrington et al 1997b;Rosche et al 2002;Aldape et al 2003;Baud et al 2005) and monocots (Hirose et al 1997;Weschke et al 2000;Aoki et al 2002; (Tegeder et al 2000b) and broad (SBP) (Harrington et al 1997a(Harrington et al , 1997b) bean cotyledons. A broad specificity amino acid transporter (PsAAP1) co-expresses with the sucrose transporter (PsSUT1) in pea cotyledons (Tegeder et al 1999(Tegeder et al , 2000a.…”

Section: Pathways Of Nutrient Transport In Filial Tissuesmentioning

confidence: 99%

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Review: Nutrient loading of developing seeds

Zhang

Zhou

Dibley

et al. 2007

Functional Plant Biol.

181

183

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Abstract. Interest in nutrient loading of seeds is fuelled by its central importance to plant reproductive success and human nutrition. Rates of nutrient loading, imported through the phloem, are regulated by transport and transfer processes located in sources (leaves, stems, reproductive structures), phloem pathway and seed sinks. During the early phases of seed development, most control is likely to be imposed by a low conductive pathway of differentiating phloem cells serving developing seeds. Following the onset of storage product accumulation by seeds, and, depending on nutrient species, dominance of path control gives way to regulation by processes located in sources (nitrogen, sulfur, minor minerals), phloem path (transition elements) or seed sinks (sugars and major mineral elements, such as potassium). Nutrients and accompanying water are imported into maternal seed tissues and unloaded from the conducting sieve elements into an extensive post-phloem symplasmic domain. Nutrients are released from this symplasmic domain into the seed apoplasm by poorly understood membrane transport mechanisms. As seed development progresses, increasing volumes of imported phloem water are recycled back to the parent plant by process(es) yet to be discovered. However, aquaporins concentrated in vascular and surrounding parenchyma cells of legume seed coats could provide a gated pathway of water movement in these tissues. Filial cells, abutting the maternal tissues, take up nutrients from the seed apoplasm by membrane proteins that include sucrose and amino acid/H + symporters functioning in parallel with non-selective cation channels. Filial demand for nutrients, that comprise the major osmotic species, is integrated with their release and phloem import by a turgorhomeostat mechanism located in maternal seed tissues. It is speculated that turgors of maternal unloading cells are sensed by the cytoskeleton and transduced by calcium signalling cascades.

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Section: Cellular Site(s) Of Exchange To the Seed Apoplasmmentioning

confidence: 68%

Section: Sucrose and Amino Acidsmentioning

confidence: 99%

Section: Pathways Of Nutrient Transport In Filial Tissuesmentioning

confidence: 99%

See 1 more Smart Citation

Review: Nutrient loading of developing seeds

Zhang

Zhou

Dibley

et al. 2007

Functional Plant Biol.

181

183

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“…Nutrient movement to and from seed storage sites involves transport between symplasmically isolated compartments and hence transport across plasma membranes of neighboring cells. For developing seeds, Suc uptake into filial tissues (cotyledons or endosperm) is mediated by plasma membrane Suc/H 1 symporters (Suc transporter [SUT]) localized to their outer cell layers in both monocots (Bagnall et al, 2000;Weschke et al, 2000;Furbank et al, 2001) and dicots (Harrington et al, 1997;Weber et al, 1997;Tegeder et al, 1999;Rosche et al, 2002). For germinating seeds, SUT transcripts have been detected in phloem tissues of castor bean (Ricinus communis;Bick et al, 1998) and rice (Oryza sativa; Matsukura et al, 2000).…”

mentioning

confidence: 99%

Pathway of Sugar Transport in Germinating Wheat Seeds

et al. 2006

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Three homeologous genes encoding a sucrose (Suc) transporter (SUT) in hexaploid wheat (Triticum aestivum), TaSUT1A, 1B, and 1D, were expressed in germinating seeds, where their function is unknown. All three TaSUT1 proteins were confirmed to be capable of transporting both Suc and maltose by complementation tests with the SUSY7/ura3 yeast (Saccharomyces cerevisiae) mutant strain. The role of Suc transporters in germinating grain was examined by combining in situ hybridization, immunolocalization, fluorescent dye tracer movement, and metabolite assays. TaSUT1 transcript and SUT protein were detected in cells of the aleurone layer, scutellar epidermis, scutellar ground cells, and sieve element-companion cell complexes located in the scutellum, shoot, and root. Ester loading of the membrane-impermeable fluorescent dye carboxyfluorescein into the scutellum epidermal cells of germinating seeds showed that a symplasmic pathway connects the scutellum to the shoot and root via the phloem. However, the scutellar epidermis provides an apoplasmic barrier to solute movement from endosperm tissue. Measurements of sugars in the root, shoot, endosperm, and scutellum suggest that, following degradation of endosperm starch, the resulting hexoses are converted to Suc in the scutellum. Suc was found to be the major sugar present in the endosperm early in germination, whereas maltose and glucose predominate during the later stage. It is proposed that loading the scutellar phloem in germinating wheat seeds can proceed by symplasmic and apoplasmic pathways, the latter facilitated by SUT activity. In addition, SUTs may function to transport Suc into the scutellum from the endosperm early in germination and later transport maltose.

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“…Transfer cell differentiation is coordinated with organ development and occurs at specific locations across particular developmental windows (Pate and Gunning, 1972;Harrington et al, 1997). Wall ingrowths of transfer cells form just as intensive transport starts, and provide efficient and rapid short-distance transport of solutes.…”

Section: Expression Of the Zerh21 Gene In Different Types Of Vasculamentioning

confidence: 99%

A RING Domain Gene Is Expressed in Different Cell Types of Leaf Trace, Stem, and Juvenile Bundles in the Stem Vascular System of Zinnia

et al. 2005

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The in vitro zinnia (Zinnia elegans) mesophyll cell system, in which leaf mesophyll cells are induced to transdifferentiate into tracheary elements with high synchrony, has become an established model for studying xylogenesis. The architecture of the stem vascular system of zinnia cv Envy contains three anatomically distinct vascular bundles at different stages of development. Juvenile vascular strands of the subapical region develop into mature vascular strands with leaf trace segments and stem segments. Characteristic patterns of gene expression in juvenile, leaf trace, and stem bundles are revealed by a molecular marker, a RING domain-encoding gene, ZeRH2.1, originally isolated from a zinnia cDNA library derived from differentiating in vitro cultures. Using RNA in situ hybridization, we show that ZeRH2.1 is expressed preferentially in two specific cell types in mature zinnia stems. In leaf trace bundles, ZeRH2.1 transcript is abundant in xylem parenchyma cells, while in stem bundles it is abundant in phloem companion cells. Both of these cell types show wall ingrowths characteristic of transfer cells. In addition, ZeRH2.1 transcript is abundant in some phloem cells of juvenile bundles and in leaf palisade parenchyma. The complex and developmentally regulated expression pattern of ZeRH2.1 reveals heterogeneity in the vascular anatomy of the zinnia stem. We discuss a potential function for this gene in intercellular transport processes.Modern dicot angiosperms possess a highly evolved vascular system that is periodically renewed and augmented by the activity of cambial cells (Foster and Gifford, 1959). The vascular system is patterned early in embryo development as a central cylinder, with a central core of cambium, surrounded by phloem and xylem. After germination, roots maintain the vasculature as a central strand, but, in the stem, the central vascular cylinder differentiates as a fixed number of vascular strands. This species-specific base number of vascular strands is then maintained by the plant as the minimum number of vascular strands (Esau, 1962). The arrangement of vascular strands is further elaborated by branching and anastomosis to supply lateral organs, like leaves and axillary branches.The postembryonic development of the stem vascular system results in an anatomically complex structure. Procambial strands at the apical meristem form protoxylem and protophloem elements of young bundles, which are gradually displaced by metaxylem and metaphloem as each stem segment matures. The anatomy of a mature bundle is, therefore, different from that of a young bundle (Esau, 1962). Partially occluded tracheids of protoxylem are displaced by the larger open vessels of metaxylem in the main stem, except in the vein supplies to leaves, in which tracheids are maintained (Eames and MacDaniels, 1925). Metaphloem contains companion cells and sieve elements to improve transport efficiency and fibers to provide strength.The neighboring parenchyma and companion cells of the conducting elements in the shoot also differe...

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Cell specific expression of three genes involved in plasma membrane sucrose transport in developingVicia faba seed

Cited by 82 publications

References 28 publications

Review: Nutrient loading of developing seeds

Review: Nutrient loading of developing seeds

Pathway of Sugar Transport in Germinating Wheat Seeds

A RING Domain Gene Is Expressed in Different Cell Types of Leaf Trace, Stem, and Juvenile Bundles in the Stem Vascular System of Zinnia

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