Cell specific tumor suppressor effect of Hsa-miR-1226-3p through downregulation of HER2, PIK3R2, and AKT1 genes

Mohamadzade, Zahra; Soltani, Bahram Mohammad; Ghaemi, Zahra; Hoseinpour, Parisa

doi:10.1016/j.biocel.2021.105965

Cited by 10 publications

(11 citation statements)

References 29 publications

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“…Quantitative real-time PCR was performed using 1 μg of RNA and SuperScript VILO Master Mix (Thermo Fisher Scientific, Waltham, MA, USA). The expression of each gene was quantified using the StepOnePlus real-time PCR system (Thermo Fisher Scientific) and Fast SYBR Green Master Mix (Thermo Fisher Scientific) with the following primers: CDKN1A , forward 5′-TGTCCGTCAGAACCCATGC-3′, reverse 5′-CCAGTTGGTAACAATGCCATGT-3′ [ 57 ]; TP53 , forward 5′-TAACAGTTCCTGCATGGGCGGC-3′, reverse 5′-AGGACAGGCACAAACACGCACC-3′ [ 58 ]; ACTA2 , forward 5′-GTGTTGCCCCTGAAGAGCAT-3′; reverse 5′-GCTGGGACATTGAAAGTCTCA-3′; COL1A1 , forward 5′-GAGGGCCAAGACGAAGACATC-3′, reverse 5′-CAGATCACGTCATCGCACAAC-3′; COL4A1 , forward 5′-GGACTACCTGGAACAAAAGGG-3′, reverse 5′-GCCAAGTATCTCACCTGGATCA-3′; COL5A1 , forward 5′-GCCCGGATGTCGCTTACAG-3′, reverse 5′-AAATGCAGACGCAGGGTACAG-3′; CXCL1 , forward 5′-GATTGTGCCTAATGTGTT-3′, reverse 5′-ATCCAGATTGAACTAACTTG-3′; CXCL2, forward 5′-GGGCAGAAAGCTTGTCTCAA-3′, reverse 5′-GCTTCCTCCTTCCTTCTGGT-3′ [ 59 ]; CXCL3 , forward 5′-CGCCCAAACCGAAGTCATAG-3′, reverse 5′-GCTCCCCTTGTTCAGTATCTTTT-3′ [ 60 ]; and GAPDH , forward 5′-GGCGTCTTCACCACCATGGAG-3′, reverse 5′-AAGTTGTCATGGATGACCTTGGC-3′ [ 12 ]. The mRNA expression levels were normalized to GAPDH .…”

Section: Methodsmentioning

confidence: 99%

Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines

Takikawa

Hamada

Matsumoto

et al. 2022

IJMS

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Interactions between pancreatic cancer cells and pancreatic stellate cells (PSCs) play an important role in the progression of pancreatic cancer. Recent studies have shown that cellular senescence and senescence-associated secretory phenotype factors play roles in the progression of cancer. This study aimed to clarify the effects of senescence-induced PSCs on pancreatic cancer cells. Senescence was induced in primary-cultured human PSCs (hPSCs) through treatment with hydrogen peroxide or gemcitabine. Microarray and Gene Ontology analyses showed the alterations in genes and pathways related to cellular senescence and senescence-associated secretory phenotype factors, including the upregulation of C-X-C motif chemokine ligand (CXCL)-1, CXCL2, and CXCL3 through the induction of senescence in hPSCs. Conditioned media of senescent hPSCs increased the proliferation—as found in an assessment with a BrdU incorporation assay—and migration—as found in an assessment with wound-healing and two-chamber assays—of pancreatic cancer AsPC-1 and MIAPaca-2 cell lines. SB225002, a selective CXCR2 antagonist, and SCH-527123, a CXCR1/CXCR2 antagonist, attenuated the effects of conditioned media of senescent hPSCs on the proliferation and migration of pancreatic cancer cells. These results suggest a role of CXCLs as senescence-associated secretory phenotype factors in the interaction between senescent hPSCs and pancreatic cancer cells. Senescent PSCs might be novel therapeutic targets for pancreatic cancer.

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Section: Methodsmentioning

confidence: 99%

Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines

Takikawa

Hamada

Matsumoto

et al. 2022

IJMS

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“…miRNAs can act as oncogenes or tumor suppressors depending on their target genes. miR-20b-5p suppresses tumorigenesis by targeting cyclin D1 in colon cancer ( 11 ), while miR-1226-3p induces apoptosis by downregulating the phosphatidylinositol 3-kinase/protein kinase B pathway in breast cancer ( 12 ). These studies have suggested that tumor-suppressor miRNAs are key to understanding the molecular mechanisms underlying cancer progression.…”

Section: Introductionmentioning

confidence: 99%

miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer

et al. 2022

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MicroRNAs are key regulators of gene expression in tumorigenesis. In this study, we investigated the tumor-suppressive function of miR-31-3p. Analysis of the Gene Expression Omnibus database revealed that the expression of miR-31-3p in prostate cancer tissues is lower than that in adjacent normal tissues from patients with prostate cancer. Moreover, miR-31-3p induces apoptosis in DU145, PC-3, and LNCap prostate cancer cells, while those transfected with miR-31-3p exhibit significantly decreased cell proliferation, migration, invasiveness, and tumor sphere-forming ability, as determined using the cell counting kit-8, transwell, and sphere-forming assays. Further analysis revealed that GABBR2 is a direct target of miR-31-3p. Within a DU145 xenograft murine model, intratumoral injection of a miR-31-3p mimic suppresses tumor growth. Taken together, the findings of this study suggest that miR-31-3p performs a novel tumor-suppressive function in prostate cancer and may represent a novel target for anti-prostate cancer miRNA therapeutics.

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“…AKT1, CASP3, PTGS2, JUN can participate in apoptosis, inflammation, and tumour occurrence (Mohamadzade et al. 2021 ; Liang et al. 2021 ; Pietruszewska et al.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology-based analysis of the mechanism of Saposhnikovia divaricata for the treatment of type I allergy

Wang

et al. 2022

Pharmaceutical Biology

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Context Saposhnikovia divaricata (Turcz.) Schischk (Apiaceae) (SD) has various pharmacological activities, but its effects on type I allergy (TIA) have not been comprehensively studied. Objective This study evaluates the treatment and molecular mechanisms of SD against TIA. Materials and methods The effective components and action targets of SD were screened using TCMSP database, and allergy-related targets of SD were predicted using GeneCards and OMIM database. The obtained target intersections were imported into David database for GO analysis, and used R software to perform KEGG analysis. The RBL-2H3 cells sensitised by DNP-IgE/DNP-BSA were treated with different concentrations of SD (root decoction, 0.5, 1, and 2 mg/mL), prim- O -glucosylcimifugin (POG, 10, 40, and 80 μg/mL) and the positive control drug–ketotifen fumarate (KF, 30 μM) for 12 h, then subjected to cell degranulation and qPCR analysis. Results Eighteen active compounds of SD and 38 intersection targets were obtained: TIA-related signal pathways mainly include calcium signal pathway, PI3K–Akt signal pathway and MAPK signal pathway. Taking the β-Hex release rate of the model group as the base, the release rate of SD and POG in high dose groups were 43.79% and 57.01%, respectively, which were significantly lower than model group ( p < 0.01), and significantly lower than KF group (63.83%, p < 0.01, p < 0.05). SD and POG could down-regulate the expression of related proteins in the Lyn/Syk, PI3K/AKT and MAPK signalling pathways. Discussion and conclusion Saposhnikovia divaricata could inhibit IgE-induced degranulation of mast cells, providing a scientific basis for further research and clinical applications of SD in TIA treatment.

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Cell specific tumor suppressor effect of Hsa-miR-1226-3p through downregulation of HER2, PIK3R2, and AKT1 genes

Cited by 10 publications

References 29 publications

Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines

Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines

miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer

Network pharmacology-based analysis of the mechanism of Saposhnikovia divaricata for the treatment of type I allergy

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