Cell surface elemental composition of Microcystis aeruginosa: high-Si and low-Si subpopulations within the water column of a eutrophic lake

Sigee, David C.; Levado, E.

doi:10.1093/plankt/22.11.2137

Cited by 21 publications

(16 citation statements)

References 16 publications

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“…During the period of suspension, cells were checked visually under a light microscope to ensure no adverse effects such as cell bursting or leaking of organic material occurred. No loss of cell integrity was observed, which is in line with previous X-ray microanalysis studies (Sigee et al, 1999a, b;Sigee & Levado, 2000) where no detectable change in cellular ionic content could be observed during short-term distilled water treatment. Droplets of the phytoplankton concentrate were then deposited on 'Low-e' reflectance slides (Kevley Technologies, Chesterland, USA), air-dried under sterile laminar flow at room temperature and stored in a desiccator until analysis.…”

Section: Methodssupporting

confidence: 91%

“…Similar intracellular chemical heterogeneity has been demonstrated in studies of elemental composition using X-ray microanalysis (XRMA) in a variety of lake cyanobacteria (El-Bestawy et al, 1996;Sigee et al 1999b, Sigee & Levado, 2000. Both FTIR and XRMA studies thus indicate chemical heterogeneity within lake cyanobacteria.…”

Section: Molecular Heterogeneity Within Micropopulationssupporting

confidence: 62%

“…Along with other biophysical techniques such as magnetic resonance imaging, chemoluminescence (Villareal & Lipschultz, 1995) and X-ray microanalysis (Sigee et al, 1999a, b;Sigee & Levado, 2000), Fourier-transform infrared (FTIR) microspectroscopy can detect and quantify a range of chemical groups within phytoplankton microsamples. These biophysical techniques have the potential to make diagnoses, identify organisms and obtain information on physiological responses in situ (Sacksteder & Barry, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Molecular heterogeneity inAphanizomenon flos-aquaeandAnabaena flos-aquae(Cyanophyta): a synchrotron-based Fourier-transform infrared study of lake micropopulations

Dean

Sigee

2006

European Journal of Phycology

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Synchrotron-based Fourier-transform infrared (FTIR) microspectroscopy was used to characterize micropopulations of the cyanobacteria Anabaena flos-aquae and Aphanizomenon flos-aquae in mixed phytoplankton samples taken from the epilimnion and hypolimnion of a stratified eutrophic lake (Rostherne Mere, UK). All FTIR spectra (normalized to amide I) showed a closely similar sequence of 10 bands over the wavenumber range 4,000-900 cm À1 and were assigned to a range of vibrationally active chemical groups. Interspecific differences between Anabaena and Aphanizomenon were revealed using correlation and multivariate analysis (principal component analysis, PCA) of the set of individual band intensities. Differences between species (separately for epilimnion and hypolimnion) were also analysed by comparing band intensity means, and by performing PCA over a broad spectral region (1,750-900 cm À1). Comparison of intensity means showed that spectra obtained from epilimnetic algae had significant differences in bands 5-7 and in bands 4, 9 and 10. However, in the spectra obtained from the hypolimnion algae, a different pattern was observed, with significant differences between the species restricted to bands 7-10. PCA of the spectral region 1,750-900 cm À1 also demonstrated interspecific differences in relation to carbohydrate and protein concentrations, with Anabaena displaying higher carbohydrate levels in both the epilimnion and hypolimnion. Intraspecific comparisons of band intensity means for epilimnion and hypolimnion subpopulations demonstrated clear differences in Aphanizomenon, where significant differences were observed between epilimnion (higher carbohydrate concentration compared with protein) and hypolimnion (lower carbohydrate concentration compared with protein) subpopulations. Anabaena however showed only minor differences. PCA comparison of the spectral region 1,750-900 cm À1 , followed by data visualization using scores plots and loading plots, also demonstrated quantitative differences.Scores plots resolved the epilimnion and hypolimnion subpopulations in Aphanizomenon (but not Anabaena), with the loading plot showing large differences in carbohydrates, supporting the analysis using discrete peak intensities.

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Section: Methodssupporting

confidence: 91%

Section: Molecular Heterogeneity Within Micropopulationssupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

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Molecular heterogeneity inAphanizomenon flos-aquaeandAnabaena flos-aquae(Cyanophyta): a synchrotron-based Fourier-transform infrared study of lake micropopulations

Dean

Sigee

2006

European Journal of Phycology

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“…The dialyzed FEPS exhibited strong interaction with metal ions (Table 1 and Table 2). The high capability of FEPS to bind Ca, Mg, K and Na is consistent with the surface element composition of single cells of field-grown M. aeruginosa determined by energy-dispersive X-ray microanalysis (SIGEE and LEVADO, 2000). Also, it is in agreement with the previous reports on EPS from lab-cultured cyanobacteria M. aeruginosa K-3A and M. flos-aquae C3-40 (Table 2) (NAKAGAWA et al, 1987;PLUDE et al, 1991).…”

Section: Chemical Composition Of Fepssupporting

confidence: 91%

Microbial Degradation and Preliminary Chemical Characterization of Microcystis Exopolysaccharides from a Cyanobacterial Water Bloom of Lake Taihu

Cai

Shi

et al. 2009

International Review of Hydrobiology

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A bloom biomass composed mainly of Microcystis spp. was harvested from Lake Taihu, China. Exopolysaccharides (FEPS, exopolysaccharides from field-grown Microcystis spp.) were extracted from the bloom mass with 3% glutaraldehyde. FEPS contained 5.4% protein and 6.9% uronic acid (glucuronic acid and galacturonic acid). The neutral sugar composition of FEPS consisted of rhamnose, arabinose, mannose, glucose and galactose in a relative molar ratio of 4.8 : 4.2 : 2.1 : 3.7 : 1.0, respectively. The di alyzed FEPS exhibited strong interaction with metal ions. Besides macromineral elements Ca, Mg, Na and K, micromineral elements Zn, Mn, Sr, Fe, Cu and Al were also detected in FEPS. The composition of FEPS is discussed with respect to its possible effects on colony formation, and bioavailability and cycling of metals in the lake. FEPS was able to be degraded rapidly at a rate of 50% in 18 d in the beginning at 25 °C after inoculation of the natural bacterial community from the bloom zone, indicating that FEPS could be recycled in nature. IntroductionExopolysaccharides (EPS) compose most of phytoplanktonic extracellular material (GREMM and KAPLAN, 1997). EPS play a significant role in the microbial food web, metal complexation, and formation of aggregates and transparent exopolymeric particles in the aquatic ecosystem (GROSSART and SIMON, 1997;GIROLDO et al., 2003;WOTTON, 2005).Phytoplanktonic EPS supply a carbon source for the heterotrophic community, especially for planktonic bacterial populations (MYKLESTAD, 1995). The mineralization of essential nutrients during bacterial utilization of the EPS could also enhance phytoplanktonic growth (NAEEM et al., 2000). Microbial degradation of several algal EPS has been demonstrated in the literature. EPS from lab-grown freshwater algae Cryptomonas tetrapyrenoidosa (Cryptophyta), Thalassiosira sp. (Bacillariophyta) and Staurastrum orbiculare (Chlorophyta) can be degraded completely by the natural bacterial community (GIROLDO et al., 2003; * Corresponding author 646 P. LI et al.

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“…Si is a macronutrient in many freshwater systems, required in large amounts for diatom cell wall synthesis (Martin-Jézéquel, 2000), but with known associations to both Al (Duan & Gregory, 1998;Sigee & Levado, 2000) and transition metals (Ellwood & Hunter, 2000;Davis et al, 2002). The occurrence and statistical associations of these elements are important at all levels within the ecosystem-reflecting availability in the aquatic medium, differential uptake into phytoplankton and changes during biomass transfer to zooplankton.…”

Section: Introductionmentioning

confidence: 99%

Aluminium, silicon and transition metal dynamics in a non-polluted lake: aquatic concentrations and phytoplankton uptake

2008

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This study was carried out to monitor and relate seasonal Al, Si and transition metal (Mn, Fe, Ni and Cu) concentrations in lake water and phytoplankton within an unpolluted lake. Lake water concentrations of Al and Mn showed seasonal (high winter-low summer) variation. The clear annual correlation pattern had significant links among transition metals, but not Si-Al. Within phytoplankton biomass, separate analyses of acid (soluble) and carbonate (insoluble) constituents showed that most elements (Si, Ni, Cu and Fe) occurred in bound form, but insoluble Al and Mn concentrations were \50% total. Diatom abundance was characterised by high Si and low Al biomass concentration, with substantial amounts of Si also occurring during phases of bluegreen dominance. The elemental correlation pattern of phytoplankton acid digests (soluble material) was different from carbonate digests, which had significant correlations among the three transition metals (Mn, Ni, and Cu)-but no relationships involving Si, Al and Fe. Data show that phytoplankton biomass accumulates high levels of Al, Si and transition metals, irrespective of taxonomic composition. The different correlation patterns seen for aquatic, soluble biomass and insoluble biomass elemental concentrations indicate that external availability, soluble uptake and insoluble deposition are distinct aspects of the pelagic ecosystem.

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Cell surface elemental composition of Microcystis aeruginosa: high-Si and low-Si subpopulations within the water column of a eutrophic lake

Cited by 21 publications

References 16 publications

Molecular heterogeneity inAphanizomenon flos-aquaeandAnabaena flos-aquae(Cyanophyta): a synchrotron-based Fourier-transform infrared study of lake micropopulations

Molecular heterogeneity inAphanizomenon flos-aquaeandAnabaena flos-aquae(Cyanophyta): a synchrotron-based Fourier-transform infrared study of lake micropopulations

Microbial Degradation and Preliminary Chemical Characterization of Microcystis Exopolysaccharides from a Cyanobacterial Water Bloom of Lake Taihu

Aluminium, silicon and transition metal dynamics in a non-polluted lake: aquatic concentrations and phytoplankton uptake

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